Skip to main content
Download PDF

Effects of graded levels of microbial fermented or enzymatically treated dried brewer’s grains on growth, digestive and nutrient transporter genes expression and cost effectiveness in broiler chickens

BMC Veterinary Research volume 16, Article number: 424 (2020) Cite this article

Abstract

Background

Poultry feed consists mainly of conventional grains and protein supplements, however, using treated unconventional agro-industrial by-products as replacements of corn soybean-based diet can minimize production costs and improve productivity. Therefore, in this study, the effects of fermented or enzymatically treated dried brewer grains (DBG) on growth, expression of digestive enzymes and nutrient transporters genes and the profitability of the rations were evaluated. A total of 1600 one-day-old Ross 308 broiler chicks were randomly distributed in 2 × 4 factorial arrangement (eight treatments with ten replicates, 20 birds/replicate). Experimental diets included two controls; negative control (basal corn-soybean diet; NC) and positive control (basal corn-soybean diet with exogenous enzymes; PC), and six diets in which basal diet was replaced by three levels of fermented DBG (FDBG; 5, 10 or 15%), or enzyme-treated DBG (DBG 5, 10 or 15%+Enz), for 38 days.

Results

The results described that feeding FDBG (three levels) or DBG5%+Enz improved (P < 0.05) BW gain and feed efficiency of broilers. Also, feeding FDBG10% yielded the best improvement in weight gain (10%), compared to NC group. Increasing the inclusion levels of DBG either fermented or enzymatically treated up-regulated (p < 0.01) expression of digestive-genes in proventriculus (PGC and PGA5, range 1.4–1.8 fold), pancreas (AMY2A, PNLIP, CELA1, and CCK; range 1.2–2.3 fold) and duodenum (CAT1, CAT2, GLUT1, GLUT2, LAT1, Pep1; range 1.3-3 fold) when compared to NC group. Feeding treated DBG significantly increased (p < 0.05, range 4.5–13.6%) gizzard relative weight compared to NC and PC groups. An additional benefit was lower (p < 0.01) cholesterol content from 66.9 mg/100 mg (NC) to 62.8 mg/100 mg (FDBG5 or 10%) in thigh meat. Furthermore, the least cost feed/kg body gain was achieved in FDBG10% and DBG5%+Enz groups, with approx. 16% reduction compared to NC cost, leading to increasing the income gross margin by 47% and 40% in FDBG10% and DBG5%+Enz groups, respectively.

Conclusions

Substitution of corn-soybean based diet with 10% FDBG or 5% DBG+Enz resulted in better growth and higher economic efficiency of broilers chickens.

Background

In commercial poultry, the feeding costs of broilers contribute up to 70% of the total production costs. And, since, global feed prices are increasing, it is important to explore alternative or unconventional feed ingredients to achieve cost-effective poultry production [1]. However, the high fiber and low protein contents and presence of antinutritional factors (ANF) in unconventional feed represent a critical challenge for their use in poultry feed. One of the most prominent plant by-products arise mainly from oilseed mills and brewing industries [2]; called dried brewer’s grains (DBG). The latter could be an appropriate low-cost replacer for traditional feedstuffs (e.g., corn and soybean meal), as it contains a fair amount of feed residues following the brewing process [3,4,5,6]. It consists of around 20% crude protein, 6% ether extract, 15% crude fiber and 4% ash, in addition it has an adequate amount of essential AA (0.4% methionine, 0.9% lysine, 1.2% phenylalanine, 0.4% tryptophan, 1.1% threonine and 1.6% valine) [7].

However, the use of DBG in poultry feed has certain limitations such as elevated moisture content, non-starch polysaccharides (NSP) and tannins hindering feed utilization and inhibiting the digestive enzymes and nutrients absorption [8]. Adding different exogenous enzymes is one option to mitigate ANF and improve DBG utilization, in addition to supporting bird’s endogenous enzymes and nutrient absorption thus improving digestibility and availability of nutrients [9, 10]. However, supplementation of exogenous enzymes in poultry feed still have limitations which reduce their beneficial effects, such as the wide range of pH along bird’s GIT (while their optimum pH is between 4 and 6), the shorter time for digesta retention in proximal GIT (their main site of action) and the possibility of hydrolysis by endogenous proteolytic enzymes [11].

The other possible strategy for improving DBG quality in broiler diet is through the fermentation approach. Fermentation is a dynamic process containing microorganisms, substrates and environmental conditions to transform complex substrates into simpler ones [12]. As the fermentation intensity depends on the number of microorganisms, thus adding fungi or bacterial strains can be a favorable aid during fermentation. It is known that fermentation improves microbial and nutritional quality of feedstuffs by increasing protein and lipids and lowering fiber content [13]. As well as, improving the availability of vitamins and AAs [14, 15] and reducing several ANF and toxic compounds [16]. Fermentation has also been proven to enhance nutrient digestibility of organic matter, fiber and calcium [17], and the palatability of feedstuff [18]. In addition, Tan et al. [19] confirmed an enhanced nutritional content of fermented DBG through biological fermentation by adding certain fungi and bacteria.

The nature of broiler’s diet can modulate the expression of important genes associated with nutrient transport and digestive enzymes [20, 21]. In fact, improving nutrient transport, through the up-regulation of the transporter encoding genes, lead to accelerated nutrient entry into the intestinal epithelium and then to the circulation [22]. It is identified that transporters of glucose (GLUT1 and GLUT2), cationic amino acid (CAT1 and CAT2) and peptide (PepT1 and PepT2) in the intestinal epithelium are associated with the capacity of nutrient absorption [21, 23,24,25].

The effects of different DBG treatments (fermentation or added enzymes) on expression of genes controlling digestive enzymes and nutrients transporters in broilers remains not fully understood. In addition, the economic efficiency of replacing costly conventional feed ingredients should promote the use of low-priced unconventional fermented feedstuffs as DBG in broiler diets. Therefore, the present study investigated the impact of feeding different levels of DBG either fermented or enzyme-treated on the gene expression of selected digestive enzymes and nutrient transporters, growth performance and economic efficiency of broiler chickens.

Results

Growth indices

The highest body weight and weight gain (P < 0.05) were observed in FDBG10% group followed by FDBG5% and DBG5%+Enz groups, Table 1. The lowest total feed intake (P < 0.05) was observed in group fed FDBG15% followed by groups fed on DBG10%+Enz and negative control. The feed conversion ratio (FCR) was improved (P < 0.05) in groups fed FDBG5%, FDBG10% and DBG5%+Enz when compared with NC and PC groups.

Table 1 Effects of different levels of fermented or enzymatically treated dried brewers’ grains on growth performance and carcass traits of broilers1
Full size table

Carcass traits

The highest dressing percentage (P < 0.05) was observed in groups fed FDBG10% and DBG5%+Enz, Table 1. Feeding on DBG either fermented or enzymatically treated significantly increased (P < 0.05) gizzard relative weight. Feeding of broiler chicks on treated DBG had no effect on the relative weights of liver, spleen, thymus, bursa and abdominal fat.

Nutrient transporter and digestive enzyme genes expression

Proventriculus pepsinogen (PGC and PGA5) genes expression (Fig. 1)

The upregulation of proventriculus pepsinogen (PGC and PGA5) genes was more prominent (P < 0.01) in groups with higher levels of DBG either fermented or enzymatically treated when compared with the NC and PC groups.

Fig. 1
figure 1

Effects of different level of fermented or enzymatically treated dried brewers’ grains on expression of proventriculus gene (PGC and PGA5). a-c Means within the same column carrying different superscripts are significantly different at P < 0.05

Full size image

Pancreatic genes expression, AMY2A and PNLIP (Fig. 2a), CELA1 and CCK (Fig. 2b)

The mRNA expression of pancreatic AMY2A, PNLIP and CELA1 was upregulated (p < 0.01) in response to all inclusion levels of treated DBG. The higher expression of AMY2A gene was more prominent in groups fed higher levels of FDBG. Regarding CCK expression level, groups fed FDBG10% and 15% had the highest expression level while FDBG5% and enzymatically treated DBG groups had the intermediate expression levels and control groups had the lowest expression level.

Fig. 2
figure 2

Effects of different level of fermented or enzymatically treated dried brewers’ grains on expression of pancreatic genes (AMY2A, PNLIP; (a), CCK and CELA1; (b). a-cMeans within the same column carrying different superscripts are significantly different at P < 0.05

Full size image

Duodenal genes expression, GLUT1 and GLUT2, (Fig. 3a); PepT1 and PepT2 (Fig. 3b) and CAT1, CAT2 (Fig. 4a) and LAT1 (Fig. 4b)

The mRNA expression of GLUT1, GLUT2, were up regulated in response to the dietary inclusion of treated DBG. The higher levels of Broilers fed on Fermented or enzymatically treated DBG or PC significantly up-regulated (P < 0.01) the CAT1, CAT2, while PepT2 expression not affected by feeding on treated DBG (P > 0.05). Increasing inclusion of fermented or enzymatically treated DBG significantly up-regulated (P < 0.01) LAT1 gene expression. Moreover, the effect of fermentation on upregulation of previous genes was more prominent than enzymes treatment. Increasing the inclusion levels from DBG either fermented or enzymatically treated up-regulated (P  < 0.05) the expression of GLUT1, GLUT2 genes than in control positive group.

Fig. 3
figure 3

Effects of different level of fermented or enzymatically treated dried brewers’ grains on expression of duodenal genes, GLUT1, GLUT2; (a) and PEPT1 and PEPT2; (b). a-bMeans within the same column carrying different superscripts are significantly different at P < 0.05

Full size image
Fig. 4
figure 4

Effects of different level of fermented or enzymatically treated dried brewers’ grains on expression of duodenal genes (CAT1, CAT2; (a) and LAT1; (b)). a-cMeans within the same column carrying different superscripts are significantly different at P < 0.05

Full size image
Chemical composition of meat

The moisture, crude protein and cholesterol contents of breast meat were not different among all treatments, Table 2. However, the groups fed on FDBG5% and FDBG10% had the lowest cholesterol content (P < 0.05) in thigh meat

Table 2 Effects of different levels of fermented or enzymatically treated dried brewers’ grains on chemical composition of meat
Full size table

.

Serum biochemical parameters

The concentrations of total protein, albumin, globulin, triglyceride, ALT and AST were not affected (P > 0 0.05) by any of the experimental diets, Table 3. However, the serum concentrations of total cholesterol and LDL were significantly lowered (P < 0.05) in broilers fed FDBG when compared with other groups.

Table 3 Effects of different levels of fermented or enzymatically treated dried brewers’ grains on some serum biochemical parameters
Full size table
Economic indices

Total feed cost and total cost decreased significantly (P < 0.05) with increasing DBG levels, either fermented or mixed with enzymes, Table 4. The highest values of total expenses were recorded in NC (2.71$) and PC (2.80$) groups. While the highest gross margin (P < 0.05) was evident in broilers fed FDBG10% (1.58$) followed by DBG5%+Enz (1.50$). The lowest cost/kg BW gain of broilers and highest cost benefit ratio were achieved in groups fed FDBG10% and DBG5%+Enz.

Table 4 Effects of different level of fermented or enzymatically treated dried brewers’ grains on the economic indices
Full size table

Discussion

Microbial fermentation is a recent low-cost method to enhance the nutritional value of unconventional feed ingredients for broiler chickens. Also, there is a growing interest in the introduction of fermented feed to broiler diets due to its positive impacts on the gut health and growth performance [26, 27]. Thus, subjecting unconventional feed as DBG to microbial fermentation may be a sound alternate to improve DBG nutritive value. In the current study, microbial fermentation improved nutritive value of DBG which allowed its inclusion up to 10% in the formulated diet and led to better broiler’s performance. Similar studies have reported that microbial fermentation is an effective method to degrade ANFs and provide probiotics in feed, thus enhancing the nutritional quality and improving animal growth [28,29,30]. Improved nutritional quality due to fermentation process is through increasing crude protein and reducing crude fiber contents [29].

Therefore, increased protein content in fermented DBG may arise from (1) synthesis of microbial proteins and enzymes during fermentation [31], (2) increasing microbial population which is composed mainly from protein [32], and (3) enhancing the activities of proteolytic microorganisms which causes an increase in free amino acids and peptides in fermented products [33]. Also, fiber reduction after fermentation is due to increased microbial population which produce fiber degrading enzymes [34]. Also, addition of B. subtilis during fermentation enhanced growth and proliferation of lactic acid bacteria (LAB) and in turn reduced pH thus inhibited the growth of pathogenic bacteria [30]. Moreover, during anerobic fermentation process, the addition of S. cerevisiae was preferred due to its greater pH lowering capacity [35] and enzyme producing ability as β -glucanase, phytase and invertase [36,37,38].

The improved BW and FCR of broiler chickens during the whole grow-out period (d 1–38) in groups fed FDBG (up to 10%), is a reflection to the improved nutritional properties of FDBG compared to enzymatically treated DBG. Similarly, recent reports showed that the fermentation help in the production of functional feeds that can improve the microecology and health of broiler’s gut and their productive performance [39]. In addition, fermentation was associated with a high concentration of organic acids which increased the number of LAB leading to lowered gastric pH and pathogenic microbial activity [17, 40, 41]. All these previous characteristics of fermented feed should prevent pathogenic feed contamination prior to feeding [42], protect chicken gastrointestinal health [43, 44] and improve their growth [45].

Adding selected microbial strains as B.subtilis, L. rhamnosus and S. cerevisiae during fermentation of DBG not only improved DBG nutritional value but also enhanced birds growth rate. In accordance with our results, microbial fermentation with B. Subtilis can increase feed palatability [46], secrete digestive enzymes (proteases, lipases and amylases), stimulate good digestion and absorption of nutrients, and, produce active substances (bacitracin, polymyxin, nystatin, gramicidin) which inhibit endogenous pathogens [47]. The last authors also suggested that microbial phytase and protease enzymes may be accountable for decreasing the contents of phytic acid and allergic proteins, respectively. In addition, S. cerevisiae effects were attributed to maintaining beneficial microbial population and modifying metabolism by enhancing the activity of digestive enzymes [48].

Treating DBG with enzymes (especially the DBG5%+ENZ group) also had positive effects on improving broiler’s FCR agreeing with previous reports describing that inclusion of exogenous enzymes in conventional or unconventional broiler’s diet led to lower digesta viscosity, countered anti-nutritional factors and assisted in the development of important microbiota [49,50,51]. Also, the incorporation of multienzyme (xylanase, amylase, and protease) in broiler’s diet improved fibers utilization [52]. While, supplementing β-glucanase to barley-based diet reduced ileal viscosity and altered the concentration of short chain fatty acids in the crop and ceca thus increased broilers body weight gain [53, 54].

Increased gizzard weight observed in all DBG groups may be due to the presence of NSP in DBG, which was shown to beneficially affect nutrient utilization by (1) increased proventriculus and gizzard activity, (2) triggered hydrochloric acid and digestive enzyme secretion and (3) augmented hind gut bacterial fermentation [55]. Moreover, a well-developed gizzard musculature can produce strong reverse peristalsis contractions and increase the digesta refluxes in digestive tract thus re‐exposing the digesta to hydrochloric acid and pepsin and help their mixing [56,57,58].

The current study demonstrated that genes essential for digestion and nutrient transport were upregulated in the proventriculus, duodenum and pancreas of broilers in line with increased level of structural fiber components (by increasing DBG level; either fermented or enzymatically treated) in broiler’s diet. This effect was more prominent with FDBG than in enzymatically treated DBG. In accordance with the upregulated pepsinogen expression after feeding treated DBG in our study, it was shown that feeding on high fiber diet upregulated the expression of pepsinogen A and C in birds [55]. In addition, the high fiber content in DBG should stimulate frequent and powerful contractions of the gizzard and relocating the digesta back into the proventriculus leading to more stimulation of proventricular function, as previously reported in high fiber diets [55]. Additionally, the upregulation of pepsinogen A and C expression with higher inclusion levels of FDBG may be related to increased LAB, more lactic acid and lower pH, which accelerated the proliferation of gut epithelial cells [59, 60] and stimulated gastric chief cells to release pepsinogen [61]. While, feeding enzymatically treated DBG could enhance pepsinogen via the supplied exogenous enzymes which upregulated the expression of digestive enzymes [62]. So, in our study increasing gizzard contraction and pepsin production in proventriculus can result in a more active foregut and better nutrient digestion.

Also, in this study, the nature of diet affected the magnitude of pancreatic secretion, as high dietary carbohydrates or fat stimulated pancreatic secretions and increased amylase and lipase concentration in serum [63,64,65]. In the current study, the increased expression of pancreatic enzymes-related genes by using DBG was in accordance with previous reports which stated that insoluble fiber can increase pancreatic enzymes such as chymotrypsin [66, 67]. Similarly, oat hulls with high fiber content stimulated the secretion of pancreatic amylase and thus increased amylase activity in the jejunum [68]. Moreover, the activities of pancreatic α-amylase and lipase in broiler chickens were significantly increased by feeding wheat and barley-based diet compared to corn-based diet with enzymes supplementation [69]. Also, elevated CCK expression with increased levels of treated DBG was related to improved gut motility, gastro-duodenal reflux and secretion of pancreatic enzymes which in turn increased CCK release and improved the digestibility [70, 71]. Likewise, fermented feeds elevated pancreatic AMY2A and CCK expression which in turn increased the secretion of pancreatic amylase and cholecystokinin in broilers [44, 72]. Additionally, the upregulated expression of pancreatic lipase in all treated DBG groups led to higher lipase secretion and resulted in better fat absorption [73]. Furthermore, the probiotic properties of FDBG led to improving intestinal structure, absorptive surface area and expression of brush border enzymes, thus achieving boosted digestion and absorption [74, 75]. Equally, B. subtilis based-diet up-regulated digestive genes such as pancreatic lipase, carboxypeptidase and chymotrypsin-like elastase in the gut [76].

Nutrient absorption, in the small intestine, is mediated by transporter proteins expressed in enterocytes. Up-regulation of these transporters improved nutrient transport capability and accelerated nutrients influx into the intestinal epithelial cells and then to all body (Ruhnke et al., 2015). In our study, GLUT1, GLUT2, CAT1, CAT2 and PEPT1 were all upregulated after feeding on DBG (either fermented or enzymatically treated). In agreement with our results, the addition of exogenous dietary enzyme for broilers significantly upregulated the expression of intestinal PEPT1 and GLUT2 thus facilitated micronutrients absorption [62]. Similarly, inclusion of fiber-rich sugarcane bagasse upregulated CAT1, LAT2 and PepT2, which facilitated the bidirectional transfer of cationic amino acids, Na+−dependent neutral/cationic amino acid exchange, and di- and tri-peptides transport in intestine, respectively [55]. The increase in carbohydrates consumption led to higher expression levels of glucose transmitters which increased glucose absorption [77]. Also, the expression of GLUT2 and PEPT1 genes was upregulated after supplementation of xylanase which may indicate an improved absorption in birds [78, 79]. All this confirms that increased entry of nutrients from the intestinal lumen into the enterocyte resulted in improved tissue protein synthesis and enhanced feed utilization as previously highlighted [80].

No considerable changes were noticed in the activities of liver metabolic enzymes (ALT and AST) among different experimental groups indicating absence of any metabolic side effects. Additionally, there was no substantial impact of treated DBG on any of the serum constituents (Albumin, globulin, cholesterol, triglyceride and high-density lipoprotein), except for total cholesterol and low-density lipoprotein LDL which were decreased in FDBG groups compared to control or enzymatically treated groups. This FDBG-caused reduction in blood cholesterol levels could be through, (1) inhibiting enzymes involved in biosynthesis of cholesterol (as 3-hydroxy-3-methylglutaryl CoA reductase) (2) increasing bile acid production, or (3) decreasing cholesterol synthesis and absorption in the gastrointestinal tract [81]. In addition, fermentation-related LAB decreased Niemann-Pick C1-like 1 expression levels in enterocytes leading to less cholesterol absorption [82].

In the present study, reduced cholesterol levels in blood was also reflected in lower cholesterol contents in thigh meat, following FDBG, which agree with previous findings [83]. In addition, it may be attributed to increased total fecal excretion of cholesterol via the bile [84]. Also, similar results were found when cholesterol concentration in breast and thigh meat decreased in turkey fed on fermented feed by-products [85]. Likewise, using of 10% fermented feed in broiler diet significantly decreased fat content in breast muscle [86].

It is accepted that using of non-conventional dietary sources can reduce the feeding cost of broilers which is a main constraint for profit in broiler industry. As described in our study the feed cost and total costs were higher in control groups, which is clearly due to the costs of conventional ingredients. However, feed cost and cost/kg diet were decreased with increasing the level of DBG in the rations, this is mainly due to its lower purchase price compared to conventional ingredients in the control diet. This was reflected in better net profit and total return values in 10% FDBG and 5% DBG + enzymes groups, which showed a decrease in feed cost/kg together with better feed utilization and body weight gain compared to the remaining groups. Similarly, appreciable financial gain was reported after increasing the inclusion level of exogenous enzymes fortified dried brewer grain in the broilers diet [87]. Also, combining exogenous enzymes with non-conventional ingredients is a valid practice to reduce the cost of feeding and to allow better utilization of the non-conventional feed ingredients that are typically rich in fibers and are difficult to be utilized by poultry endogenous enzymes. This was achieved in this study in the group fed on enzymatically treated DBG at 5% inclusion level. Additionally, previous findings indicated that the total cost of production and feed cost/kg decreased with increasing BDGs level [88]. Moreover, the feed cost effectiveness in FDBG 10%, agreed with previous reports [89, 90] which reported that probiotic produced by solid substrate fermentation (SSF) is economically and environment-friendly. Furthermore, we confirm previous hypothesis that using fermented feed containing probiotics would be of economic value via improving the broiler feed conversion ratio [91]. Therefore, from an economic point of view, it is clear in our results that fermentation of DBG achieved better economic production than enzymatically treated DBG.

Conclusions

Introduction of new nutritional strategies (such as microbial fermentation) for broilers, lead to better utilization of unconventional feed as DBG and improving the revenue. Fermentation of DBG positively affected the expression of digestive and nutrient transporters genes which regulate nutrients and energy availability necessary for optimum bird-growth, more than enzymatic treated DBG. These outcomes should encourage the poultry feed industry to apply cost effective protocols by incorporating fermented unconventional products in broiler diets.

Methods

Microbial fermentation of Dried Brewer Grains

The dried brewer’s grains (DBG) were obtained from beer factory and dried for 12 h at 40 °C then finely ground, then were fermented by different microbes. Bacillus subtilis (NCIMB 15,204) was activated with nutrient broth (5 ml) at 37 °C for 24 h. Lactobacillus rhamnosus (ATCC 7469) cultures were anaerobically incubated in De Man, Rogosa and Sharpe (MRS) (Oxoid Ltd., Basingstoke, Hampshire, UK) at 37 °C for 24 h for activation. Saccharomyces cerevisae (CGMCC No. 2.1793) was activated by incubation at 37 °C for 48 h in glycerol and MRS broth. A mixed liquid culture of approximately 1 × 106 CFU/ml of each S. cerevisiae, L. rhamnosus and B. subtilis, were utilized by the same ratio and mixed with DBG. The mixture was packaged and sealed in polyethylene bags with a one-way valve to permit carbon dioxide release during fermentation. Through the initial phase of fermentation, S. cerevisae consumed the oxygen in the bag and generated significant amount of carbon dioxide. This process produced an anaerobic and acidic conditions which enabled both L. rhamnosus and B. subtilis to grow and germinate. The fermentation was held for 25 days. Samples of dried brewers’ grains (DBG) were collected for chemical analysis according to AOAC [92], and shown in Table 5.

Table 5 Chemical analysis (% on DM basis) of unfermented (UFDBG) and fermented (FDBG) dried brewers’ grains
Full size table

Birds, experimental design, and management

One-day-old male Ross 308 broiler chicks (n = 1600) were obtained from a commercial chick producer (Dakahlia Poultry, Mansora, Egypt) and weighted and randomly assigned to one of the eight experimental treatments (ten replicates/treatment, and 20 birds/replicate) in 2 × 4 factorial arrangement. Treatments consist of negative control (NC) birds received corn-soybean based diet; positive control (PC) birds received corn-soybean based diet with exogenous enzymes and in the remaining six diets, the basal diet was replaced by three levels of fermented DBG (FDBG; 5, 10 or 15%), or enzyme-treated DBG (DBG 5, 10 or 15%+Enz). A commercial multi-exogenous enzyme was added was added to PC and enzyme treated DBG diets at a concentration of 1 gm/kg diet.

The birds were raised in a naturally ventilated open house with sawdust as litter. The feed and water were available ad-libitum and the broilers were raised in floor pens over a period of 38 days. The temperature, relative humidity and lighting were adjusted following Ross 308 management guidelines [93]. All diets were offered in mash form and the basal diets were formulated (starter, grower-finisher) according to nutrition specification of Ross broiler handbook [93], Tables 6 and 7. The chemical analyses (moisture, crude protein, ether extract and crude fiber) of all feed ingredients were conducted according to AOAC [92]. A commercial multi-exogenous enzymes (KEMZYME® Plus Europa NV, Belgium), containing Xylanase, B-glucanase, cellulase, alpha-amylase and protease. After the study, all remaining birds were released.

Table 6 The ingredients and nutrient level of diets during starter stage
Full size table
Table 7 The ingredients and nutrient level of diets during grower-finisher stage
Full size table

Samples collection

At the end of experimental period. The birds euthanized by cervical dislocation and the blood samples (n = 5 per replicate) were collected in sterilized tubes from the brachial vein and the serum was separated for biochemical analyses. Following blood collection, these birds were manually defeathered and eviscerated to calculate the carcass weight together with the weights of gizzard, liver, spleen, thymus, bursa and abdominal fat. Samples (n = 5 per replicate) from breast and thigh meat were quickly obtained and stored at -20ºC until analysis of moisture, protein, and cholesterol in breast and cholesterol content in thigh. Small pieces (n = 5 per replicate), approximately 2 cm in the middle of proventriculus and pancreas and 4 cm of duodenum (distal loop) were excised, flushed with phosphate buffer saline, collected into Eppendorf cap lock tube and stored in -80ºC for subsequent RNA extraction.

Measurement of growth performance and carcass traits:

Birds and feed intake in each pen were weighed weekly and body weight gain (BWG), feed intake (FI), and feed conversion ratio (FCR) were calculated. At the end of experimental period BWG, FI and FCR were calculated for total growing period (days 1–38). The carcass weight (dressing yield) was calculated as the percentage of live weight and weight of gizzard, liver, spleen, thymus, bursa and abdominal fat was calculated as a percentage of carcass weight.

RNA Extraction, Reverse Transcription, and Quantitative Real-Time PCR

For each sample, total RNA was extracted from the proventriculus, pancreas and duodenum by RNeasy Mini Kit (Qiagen, Cat. No. 74,104) according to the manufacturer’s directions. The quantity and purity of total RNA was determined using a NanoDrop (ND-8000 spectrophotometer, Thermo Fisher Scientific, Waltham, USA). The isolated RNA of each sample was reverse transcribed using RevertAid™ H Minus kits (Fermentas Life Science, Pittsburgh, PA, USA). The cDNA (One µL) was mixed with 2x SYBR® Green PCR mix (12.5 µL), and RNase free water (5.5 µL), then 0.5 µL of each forward and reverse primer for the selected genes were added. The primers’ sequences of selected digestive and nutrient transporters genes were designed as previously described in Kheravii et al. [55], Table 8. The GAPDH was used as an internal control to normalize target gene expression levels [94]. The real-time PCR amplification was done with Rotor-Gene Q2 plex (Qiagen Inc., Valencia, CA, USA).

Table 8 Primers Sequences and target genes used for quantitative real-time PCR
Full size table

Biochemical analyses

The serum biochemical indices: total plasma proteins, albumin, globulin, total cholesterol, triglycerides, high density conjugated protein (HDL), low density protein (LDL), alanine aminotransferase (ALT) and aspartate amino transferase (AST) were measured using diagnostic kits (Spinreact, Santa Coloma, Spain).

Analysis of cholesterol content in breast and thigh meat

The moisture and protein content of breast were calculated according to AOAC [92]. The total cholesterol in breast and thigh meat was determined by gas chromatography, as previously reported by Allain et al. [95].

Economic analysis

The economic efficiency of substituting corn-soybean by fermented or enzyme-treated DBG was calculated from the input-output analysis (as per the prevailing value of the experimental diets and also the broiler body weight during the experimental period) as follows:

Total feed cost = total feed intake per bird × cost of one kg diet [96].

Feed cost/kg BW gain = feed conversion × cost of one kg diet [97].

Total expenses = total feed cost + average fixed costs [2].

Total revenue = live body weight × price/kg [98].

Gross margin = total revenue – total expenses [98].

Benefit-cost ratio = gross margin/total feed cost [99].

Statistical analyses

The data were analyzed using GLM procedure of SPSS, after confirming the homogeneity among experimental groups using Levene’s test and normality using Shapiro-Wilk’s test. Tukey’s post hoc was used to test for significant differences between the mean values. Variation in the data was expressed as standard error of the mean (SEM) and the significance was set at 0.05. Relative fold changes in the expression of target genes calculated by the 2−ΔΔCt method as described by Livak and Schmittgen [100].

Abbreviations

DBG:

Dried brewer grains

FDBG:

Fermented dried brewer grains

NC:

Negative control

PC:

Positive control

Enz:

Enzyme

PGC:

Pepsinogen C

PGA5:

Pepsinogen A

AMY2A:

Pancreatic alpha 2A amylase

PNLIP:

Pancreatic lipase

CELA1:

Chymotrypsin-like elastase family, member 1

CCK:

Cholecystokinin

CAT1:

Cationic amino acid transporter-1 (SLC7A1)

CAT2:

Cationic amino acid transporter-1 (SLC7A2)

GLUT1:

Glucose transporter-1 (SLC2A1)

GLUT2:

Glucose transporter-2 (SLC2A2)

LAT1:

L-type amino acid transporter-1

Pep1:

Peptide transporter-1 (SLC15A1)

Pep2:

Peptide transporter-2 (SLC15A2)

GAPDH:

Glyceraldahyde − 3-phosphate dehydrogenase

ANF:

Antinutritional factors

NSP:

Non-starch polysaccharides

LAB:

Lactic acid bacteria

BWG:

Body weight gain

FI:

Feed intake

FCR:

Feed conversion ratio

HDL:

High density conjugated protein

LDL:

Low density protein

ALT:

Alanine aminotransferase and

AST:

Aspartate amino transferase

GLM:

General linear method

SEM:

Standard error of the mean

AA:

Amino acids

References

  1. Al-Sagheer AA, Abd El-Hack ME, Alagawany M, Naiel MA, Mahgoub SA, Badr MM, Hussein EO, Alowaimer AN, Swelum AA (2019) Paulownia leaves as a new feed resource: Chemical composition and effects on growth, carcasses, digestibility, blood biochemistry, and intestinal bacterial populations of growing rabbits. Animals 9(3):95.

    Article  Google Scholar 

  2. Fasuyi A, Aturamu O, Lawal A (2018) Part II: Economic analyses and the growth performance of broiler finisher (29–58 day) birds on high fibre-low protein industrial plant by-products. Asian J Adv Agric Res 6(3):1–10.

    Google Scholar 

  3. Aregheore E, Abdulrazak S (2005) Estimation of organic matter digestibility and metabolizable energy content of agro-industrial wastes using in vitro gas production. Nig J Anim Prod 32(1):79–87.

    Google Scholar 

  4. Khalili A, Tabeidian SA, Toghyani M, Ghalamkari G, Bahrami Y (2011) Effect of different levels of brewer’s dried grains and enzyme on performance, protein digestibility, immune response and performance of broilers. Int J Acad Res 3(2):1153–7.

    Google Scholar 

  5. McCarthy AL (2013) Novel ingredients from brewers’ spent grain-bioactivity in cell culture model systems and bioactivity retention in fortified food products.

    Google Scholar 

  6. Radzik-Rant A, Rant W, Niżnikowski R, Świątek M, Szymańska Ż, Ślęzak M, Niemiec T (2018) The effect of the addition of wet brewers grain to the diet of lambs on body weight gain, slaughter valueand meat quality. Archiv fuer Tierzucht 61(2):245.

    Google Scholar 

  7. ME AE-H, Alagawany M, Patra A, Abdel-Latef M, Ashour E, Arif M, Farag M, Dhama K (2019) Use of brewers dried grains as an un-conventional feed ingredient in the diets of broiler chickens: a review. Adv Anim Vet Sci 7(3):218–24.

    Google Scholar 

  8. Ashour E, El-Hack MA, El-Hindawy M, Attia A, Osman A, Swelum A, Alowaimer A, Saadeldin I, Laudadio V (2019) Impacts of dietary inclusion of dried brewers’ grains on growth, carcass traits, meat quality, nutrient digestibility and blood biochemical indices of broilers. S Afr J Anim Sci 49(3):573–84.

    Article  CAS  Google Scholar 

  9. Isikwenu J (2011) Performance and economic analysis of cockerel chicks fed enzyme supplemented brewer’s dried grains groundnut cake-based diets. Agric Biol J North Am 2:47–51.

    Article  CAS  Google Scholar 

  10. Alabi O, Atteh J, Adejumo I, Ogundele O (2014) Effects of dietary levels of brewers’ dried grain supplemented with commercial enzymes on performance, nutrient retention and gastro-intestinal tract characteristics of Arbor acres broilers. Int J Agric Innov Res 2(5):818–23.

  11. Ravindran V (2013) Feed enzymes: The science, practice, and metabolic realities. J Appl Poultry Res 22(3):628–36.

    Article  CAS  Google Scholar 

  12. Niba A, Beal J, Kudi A, Brooks P (2009a) Potential of bacterial fermentation as a biosafe method of improving feeds for pigs and poultry. Afr J Biotech 8(9):1758–1767.

    CAS  Google Scholar 

  13. Sugiharto S, Yudiarti T, Isroli I (2015) Functional properties of filamentous fungi isolated from the Indonesian fermented dried cassava, with particular application on poultry. Mycobiology 43(4):415–22.

    Article  Google Scholar 

  14. Borresen C, Erica J, Henderson A, Kumar A, L Weir T, Ryan P (2012) Fermented foods: patented approaches and formulations for nutritional supplementation and health promotion. Recent Pat Food Nutr Agric 4(2):134–40.

    Article  CAS  Google Scholar 

  15. Sugiharto S, Yudiarti T, Isroli I (2016) Performances and haematological profile of broilers fed fermented dried cassava (Manihot esculenta Crantz). Trop Anim Health Prod 48(7):1337–41.

    Article  Google Scholar 

  16. Xu F, Zeng X, Ding X (2012) Effects of replacing soybean meal with fermented rapeseed meal on performance, serum biochemical variables and intestinal morphology of broilers. Asian-Australas J Anim Sci 25(12):1734.

    Article  CAS  Google Scholar 

  17. Canibe N, Jensen BB (2012) Fermented liquid feed—Microbial and nutritional aspects and impact on enteric diseases in pigs. Anim Feed Sci Technol 173(1–2):17–40.

    Article  CAS  Google Scholar 

  18. Shahowna E, Mahala A, Mokhtar A, Amasaib E, Attaelmnan B (2013) Evaluation of nutritive value of sugar cane bagasse fermented with poultry litter as animal feed. Afr J Food Sci Technol 4:106e109.

    Google Scholar 

  19. Tan YX, Mok WK, Lee J, Kim J, Chen WN (2019) Solid state fermentation of brewers’ spent grains for improved nutritional profile using bacillus subtilis wx-17. Fermentation 5(3):52.

    Article  CAS  Google Scholar 

  20. Poncet N, Taylor PM (2013) The role of amino acid transporters in nutrition. Curr Opin Clin Nutr Metab Care 16(1):57–65.

    Article  CAS  Google Scholar 

  21. Kheravii S, Morgan N, Swick RA, Choct M, Wu S-B (2018) Roles of dietary fibre and ingredient particle size in broiler nutrition. Worlds Poult Sci J 74(2):301–16.

    Article  Google Scholar 

  22. Ruhnke I, Röhe I, Goodarzi Boroojeni F, Knorr F, Mader A, Hafeez A, Zentek J (2015) Feed supplemented with organic acids does not affect starch digestibility, nor intestinal absorptive or secretory function in broiler chickens. J Anim Physiol Anim Nutr (Berl) 99:29–35.

    Article  CAS  Google Scholar 

  23. Fotiadis D, Kanai Y, Palacín M (2013) The SLC3 and SLC7 families of amino acid transporters. Mol Aspects Med 34(2–3):139–58.

    Article  CAS  Google Scholar 

  24. Chen H, Mao X, Yin J, Yu B, He J, Che L, Yu J, Huang Z, Zheng P, Michiels J (2015) Comparison of jejunal digestive enzyme activities, expression of nutrient transporter genes, and apparent fecal digestibility in weaned piglets fed diets with varied sources of fiber. J Anim Feed Sci 24(1):41–7.

  25. Röder PV, Geillinger KE, Zietek TS, Thorens B, Koepsell H, Daniel H (2014) The role of SGLT1 and GLUT2 in intestinal glucose transport and sensing. PLoS One 9(2):e89977.

    Article  CAS  Google Scholar 

  26. Alshelmani MI, Loh TC, Foo HL, Sazili AQ, Lau WH (2017) Effect of solid state fermentation on nutrient content and ileal amino acids digestibility of palm kernel cake in broiler chickens. Indian J Anim Sci 87:1135–40.

    CAS  Google Scholar 

  27. Zhang J, Zhu J, Sun J, Li Y, Wang P, Jiang R, Rahman MRT (2016) Effect of fermented feed on intestinal morphology, immune status, carcass and growth performance of Emei Black chickens. FASEB J 30(1_supplement):lb240-0.

    Google Scholar 

  28. Shi C, Zhang Y, Lu Z, Wang Y (2017) Solid-state fermentation of corn-soybean meal mixed feed with Bacillus subtilis and Enterococcus faecium for degrading antinutritional factors and enhancing nutritional value. J Anim Sci Biotechnol 8(1):50.

    Article  CAS  Google Scholar 

  29. Khempaka S, Thongkratok R, Okrathok S, Molee W (2013) An evaluation of cassava pulp feedstuff fermented with A. oryzae, on growth performance, nutrient digestibility and carcass quality of broilers. Poult Sci 51(1):71–79. https://doi.org/10.2141/jpsa.0130022.

    Article  CAS  Google Scholar 

  30. Jazi V, Boldaji F, Dastar B, Hashemi S, Ashayerizadeh A (2017) Effects of fermented cottonseed meal on the growth performance, gastrointestinal microflora population and small intestinal morphology in broiler chickens. Br Poult Sci 58(4):402–8.

    Article  CAS  Google Scholar 

  31. Zhang W-J, Xu Z-R, Zhao S-H, Sun J-Y, Yang X (2007) Development of a microbial fermentation process for detoxification of gossypol in cottonseed meal. Anim Feed Sci Technol 135(1–2):176–86.

    Article  CAS  Google Scholar 

  32. Sun H, Jiang-Wu T, Xiao-Hong Y, Yi-Fei W, Wang X, Feng J (2012) Improvement of the nutritional quality of cottonseed meal by Bacillus subtilis and the addition of papain. Int J Agric Biol 14(4):563–568.

    CAS  Google Scholar 

  33. Sun H, Yao X, Wang X, Wu Y, Liu Y, Tang J, Feng J (2015) Chemical composition and in vitro antioxidant property of peptides produced from cottonseed meal by solid-state fermentation. CyTA-J Food 13(2):264–72.

  34. Sugiharto S, Ranjitkar S (2018) Recent advances in fermented feeds towards improved broiler chicken performance, gastrointestinal tract microecology and immune responses: A review. Animal nutrition 5(1):1.

    Article  Google Scholar 

  35. Yeh RH, Hsieh CW, Chen KL (2018) Screening lactic acid bacteria to manufacture two-stage fermented feed and pelleting to investigate the feeding effect on broilers. Poult Sci 97(1):236–46.

    Article  CAS  Google Scholar 

  36. Ries EF, Alves Macedo G (2011) Improvement of phytase activity by a new Saccharomyces cerevisiae strain using statistical optimization. Enzyme Res 2011:796394.

  37. Cenamor R, Molina M, Galdona J, Sánchez M (1987) Production and secretion of Saccharomyces cerevisiae β-glucanases: differences between protoplast and periplasmic enzymes. Microbiology 133(3):619–28.

  38. Arumugam G, Sadiq AM, Nagalingam M, Panneerselvam A (2014) Production of invertase enzymes from Saccharomyces cerevisiae strain isolated from sugarcane and grape juices. Eur J Exp Biol 4(5):29–32.

    CAS  Google Scholar 

  39. Sugiharto S, Ranjitkar S (2019) Recent advances in fermented feeds towards improved broiler chicken performance, gastrointestinal tract microecology and immune responses: A review. Anim Nutr 5(1):1–10.

  40. Engberg RM, Hammersh⊘ j M, Johansen N, Abousekken MS, Steenfeldt S, Jensen BB (2009) Fermented feed for laying hens: effects on egg production, egg quality, plumage condition and composition and activity of the intestinal microflora. Br Poult Sci 50(2):228–39.

    Article  CAS  Google Scholar 

  41. Cutler S, Rasmussen M, Hensley M, Wilhelms K, Griffith R, Scanes C (2005) Effects of Lactobacilli and lactose on Salmonella typhimurium colonisation and microbial fermentation in the crop of the young turkey. Br Poult Sci 46(6):708–16.

    Article  CAS  Google Scholar 

  42. Niba A, Beal J, Kudi A, Brooks P (2009b) Bacterial fermentation in the gastrointestinal tract of non-ruminants: influence of fermented feeds and fermentable carbohydrates. Trop Anim Health Prod 41(7):1393.

    Article  CAS  Google Scholar 

  43. Missotten J, Michiels J, Dierick N, Ovyn A, Akbarian A, De Smet S (2013) Effect of fermented moist feed on performance, gut bacteria and gut histo-morphology in broilers. Br Poult Sci 54(5):627–34.

    Article  CAS  Google Scholar 

  44. Sun H, Tang J, Fang C, Yao X, Wu Y, Wang X, Feng J (2013) Molecular analysis of intestinal bacterial microbiota of broiler chickens fed diets containing fermented cottonseed meal. Poult Sci 92(2):392–401.

    Article  CAS  Google Scholar 

  45. Xie Pj H, Lx, Zhang Ch (2016) Nutrient assessment of olive leaf residues processed by solid-state fermentation as an innovative feedstuff additive. J Appl Microbiol 121(1):28–40.

    Article  CAS  Google Scholar 

  46. Gao Z, Wu H, Shi L, Zhang X, Sheng R, Yin F, Gooneratne R (2017) Study of Bacillus subtilis on growth performance, nutrition metabolism and intestinal microflora of 1 to 42 d broiler chickens. Anim Nutr 3(2):109–13.

  47. Li W, Bai J, Li Y, Qin Y, Yu D (2014) Effects of Bacillus subtilis on meat quality, nutrient digestibility and serum biochemical parameters of broilers. Chin J Vet Sci 34(10):1682–5.

    Google Scholar 

  48. Shankar PA, Premavalli K, Omprakash A, Kirubakaran JJ, Hudson G, Vairamuthu S (2017) Effect of Dietary Yeast Supplementation on Serum Biochemical Profile of Broiler Chicken. 95(6):13–15.

    Google Scholar 

  49. Horvatovic M, Glamocic D, Zikic D, Hadnadjev T (2015) Performance and some intestinal functions of broilers fed diets with different inclusion levels of sunflower meal and supplemented or not with enzymes. Braz J Poultry Sci 17(1):25–30.

  50. Almirall M, Francesch M, Perez-Vendrell AM, Brufau J, Esteve-Garcia E (1995) The differences in intestinal viscosity produced by barley and β-glucanase alter digesta enzyme activities and ileal nutrient digestibilities more in broiler chicks than in cocks. J Nutr 125(4):947–55.

    CAS  PubMed  Google Scholar 

  51. Bedford M, Cowieson A (2012) Exogenous enzymes and their effects on intestinal microbiology. Anim Feed Sci Technol 173(1–2):76–85.

    Article  CAS  Google Scholar 

  52. Singh A, Tiwari U, Berrocoso J, Dersjant-Li Y, Awati A, Jha R (2019) Effects of a combination of xylanase, amylase and protease, and probiotics on major nutrients including amino acids and non-starch polysaccharides utilization in broilers fed different level of fibers. Poult Sci 98(11):5571–81.

    Article  CAS  Google Scholar 

  53. Kalantar M, Khajali F, Yaghobfar A (2015) Different dietary source of non-starch polysaccharides supplemented with enzymes affected growth and carcass traits, blood parameters and gut physicochemical properties of broilers. Global J Anim Sci Res 3:412–8.

    Google Scholar 

  54. Zeng Q, Huang X, Luo Y, Ding X, Bai S, Wang J, Xuan Y, Su Z, Liu Y, Zhang K (2015) Effects of a multi-enzyme complex on growth performance, nutrient utilization and bone mineralization of meat duck. J Anim Sci Biotechnol 6(1):12.

    Article  CAS  Google Scholar 

  55. Kheravii S, Swick RA, Choct M, Wu S-B (2018) Upregulation of genes encoding digestive enzymes and nutrient transporters in the digestive system of broiler chickens by dietary supplementation of fiber and inclusion of coarse particle size corn. BMC Genom 19(1):208.

    Article  CAS  Google Scholar 

  56. Ferket P (2000) Feeding whole grains to poultry improves gut health. Feedstuffs 72(37):12–3.

    Google Scholar 

  57. González-Alvarado J, Jiménez-Moreno E, Valencia D, Lázaro R, Mateos G (2008) Effects of fiber source and heat processing of the cereal on the development and pH of the gastrointestinal tract of broilers fed diets based on corn or rice. Poult Sci 87(9):1779–95.

    Article  CAS  Google Scholar 

  58. Svihus B (2011) The gizzard: function, influence of diet structure and effects on nutrient availability. Worlds Poult Sci J 67(2):207–24.

    Article  Google Scholar 

  59. Pacheco W, Stark C, Ferket P, Brake J (2014) Effects of trypsin inhibitor and particle size of expeller-extracted soybean meal on broiler live performance and weight of gizzard and pancreas. Poult Sci 93(9):2245–52.

    Article  CAS  Google Scholar 

  60. Gabriel I, Mallet S, Leconte M, Travel A, Lalles J (2008) Effects of whole wheat feeding on the development of the digestive tract of broiler chickens. Anim Feed Sci Technol 142(1–2):144–62.

    Article  Google Scholar 

  61. Lan Y, Williams BA, Tamminga S, Boer H, Akkermans A, Erdi G, Verstegen MW (2005) In vitro fermentation kinetics of some non-digestible carbohydrates by the caecal microbial community of broilers. Anim Feed Sci Technol 123:687–702.

    Article  CAS  Google Scholar 

  62. Saleh AA, Ali H, Abdel-Latif MA, Emam MA, Ghanem R, El-Hamid HSA (2018) Exogenous dietary enzyme formulations improve growth performance of broiler chickens fed a low-energy diet targeting the intestinal nutrient transporter genes. PLoS One 13(5):e0198085.

    Article  CAS  Google Scholar 

  63. Brenes A, Smith M, Guenter W, Marquardt R (1993) Effect of enzyme supplementation on the performance and digestive tract size of broiler chickens fed wheat-and barley-based diets. Poult Sci 72(9):1731–9.

    Article  CAS  Google Scholar 

  64. Zhao F, Hou S, Zhang H, Zhang Z (2007) Effects of dietary metabolizable energy and crude protein content on the activities of digestive enzymes in jejunal fluid of Peking ducks. Poult Sci 86(8):1690–5.

    Article  CAS  Google Scholar 

  65. Lin P, Shih B, Hsu J (2010) Effects of different sources of dietary non-starch polysaccharides on the growth performance, development of digestive tract and activities of pancreatic enzymes in goslings. Br Poult Sci 51(2):270–7.

    Article  CAS  Google Scholar 

  66. Yokhana J, Parkinson G, Frankel T (2016) Effect of insoluble fiber supplementation applied at different ages on digestive organ weight and digestive enzymes of layer-strain poultry. Poult Sci 95(3):550–9.

    Article  CAS  Google Scholar 

  67. Bogułsawska-Tryk M (2005) Effect of different levels of cellulose in the diet on the proteolytic activity of the pancreas in broiler chickens. Folia Biol 53(4):19–23.

    Article  Google Scholar 

  68. Hetland H, Svihus B, Krogdahl Å (2003) Effects of oat hulls and wood shavings on digestion in broilers and layers fed diets based on whole or ground wheat. Br Poult Sci 44(2):275–82.

    Article  CAS  Google Scholar 

  69. Li W-F, Feng J, Xu Z-R, Yang C-M (2004) Effects of non-starch polysaccharides enzymes on pancreatic and small intestinal digestive enzyme activities in piglet fed diets containing high amounts of barley. World J Gastroenterol 10(6):856.

    Article  CAS  Google Scholar 

  70. Kheravii SK, Choct M, Swick R, Wu R (2017) Improved performance of broilers by structural manipulation of feed: Evidence from gizzard development, nutrient digestibility, gut microflora and gene expression.

    Google Scholar 

  71. Kheravii S, Swick RA, Choct M, Wu S-B (2017) Dietary sugarcane bagasse and coarse particle size of corn are beneficial to performance and gizzard development in broilers fed normal and high sodium diets. Poult Sci 96(11):4006–16.

    Article  CAS  Google Scholar 

  72. Feng J, Liu X, Xu Z, Wang Y, Liu J (2007) Effects of fermented soybean meal on digestive enzyme activities and intestinal morphology in broilers. Poult Sci 86(6):1149–54.

    Article  CAS  Google Scholar 

  73. Karásková K, Suchý P, Straková E (2015) Current use of phytogenic feed additives in animal nutrition: a review. Czech J Anim Sci 60(12):521–30.

    Article  Google Scholar 

  74. Pluske JR, Williams IH, Aherne FX. Nutrition of the neonatal pig. In: Varley M.A., editor. The neonatal pig - development and survival. Wallingford: CAB International; 1995. p. 187–235.

  75. Chiang G, Lu W, Piao X, Hu J, Gong L, Thacker P (2009) Effects of feeding solid-state fermented rapeseed meal on performance, nutrient digestibility, intestinal ecology and intestinal morphology of broiler chickens. Asian-Australas J Anim Sci 23(2):263–71.

    Article  Google Scholar 

  76. Lee K-W, Kim DK, Lillehoj HS, Jang SI, Lee S-H (2015) Immune modulation by Bacillus subtilis-based direct-fed microbials in commercial broiler chickens. Anim Feed Sci Technol 200:76–85.

    Article  CAS  Google Scholar 

  77. Ferraris RP (2001) Dietary and developmental regulation of intestinal sugar transport. Biochem J 360(2):265–76.

    Article  CAS  Google Scholar 

  78. Lee SA, Wiseman J, Masey O’Neill HV, Scholey DV, Burton EJ, Hill SE (2017) Understanding the direct and indirect mechanisms of xylanase action on starch digestion in broilers. J World’s Poultry Res 7(2):35–47.

  79. Hosseini SM, Manafi M, Nazarizadeh H (2016) Effects of xylanase supplementation and citric acid on performance, ileal nutrients digestibility, and gene expression of intestinal nutrient transporters in broilers challenged with Clostridium perfringens. The Journal of Poultry Science 54(2):149–156. https://doi.org/10.2141/jpsa.0160099.

    Article  CAS  Google Scholar 

  80. Wang Y, Gu Q (2010) Effect of probiotic on growth performance and digestive enzyme activity of Arbor Acres broilers. Res Vet Sci 89(2):163–7.

    Article  CAS  Google Scholar 

  81. Shang HM, Zhao JC, Guo Y, Zhang HX, Song H. Effects of supplementing feed with fermentation concentrate of Hericium caput-medusae (Bull.: Fr.) Pers. on cholesterol deposition in broiler chickens. Livestock Science. 2020. p. 104009.

  82. Huang Y, Zheng Y (2010) The probiotic Lactobacillus acidophilus reduces cholesterol absorption through the down-regulation of Niemann-Pick C1-like 1 in Caco-2 cells. Br J Nutr 103(4):473–8.

    Article  CAS  Google Scholar 

  83. Ashayerizadeh A, Dastar B, Shargh MS, Mahoonak AS, Zerehdaran S (2018) Effects of feeding fermented rapeseed meal on growth performance, gastrointestinal microflora population, blood metabolites, meat quality, and lipid metabolism in broiler chickens. Livestock Sci 216:183–90.

  84. Ramasamy K, Abdullah N, Wong MC, Karuthan C, Ho YW (2010) Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens. J Sci Food Agric 90(1):65–9.

    Article  CAS  Google Scholar 

  85. Drażbo A, Kozłowski K, Ognik K, Zaworska A, Jankowski J (2019) The effect of raw and fermented rapeseed cake on growth performance, carcass traits, and breast meat quality in turkey. Poult Sci 98(11):6161–9.

    Article  CAS  Google Scholar 

  86. Nie C, Zhang W, Ge W, Liu Y, Wang Y, Liu J (2015) Effect of cottonseed meal fermented with yeast on the lipid-related gene expression in broiler chickens. Braz J Poultry Sci 17(SPE):57–64.

  87. Okorie K, Ehirim F, Gabriel O, Ikpe J, Okorie R, Okoro-Ugo C (2011) Effect of exogenous enzyme fortified palm kernel meal on the performance, carcass quality and biochemical profile of finisher broiler. Glob Res J Sci 1:86–92.

  88. Adama T, Ogunbajo S, Mambo M (2007) Feed intake, growth performance and nutrient digestibility of broiler chicks fed diets containing varying levels of sorghum dried brewers’ grains. Int J Poultry Sci 6(8):592–598.

  89. El-Bendary MA (2006) Bacillus thuringiensis and Bacillus sphaericus biopesticides production. J Basic Microbiol 46(2):158–70.

    Article  CAS  Google Scholar 

  90. Shim Y, Shinde P, Choi J, Kim J, Seo D, Pak J, Chae B, Kwon I (2010) Evaluation of multi-microbial probiotics produced by submerged liquid and solid substrate fermentation methods in broilers. Asian-Australas J Anim Sci 23(4):521–9.

    Article  CAS  Google Scholar 

  91. Naji SA, Al-Zamili I, Hasan S, Al-Gharawi J (2016) The Effects of Fermented Feed on Broiler Production and Intestinal Morphology. Pertanika J Trop Agric Sci 39(4):597–607.

    Google Scholar 

  92. AOAC (2012) Official methods of analysis of AOAC International, Association of Official Analytical Chemists.

    Google Scholar 

  93. Aviagen W (2018) Ross 308: broiler’s management and nutrition specification.

    Google Scholar 

  94. Li YP, Bang DD, Handberg KJ, Jorgensen PH, Zhang MF (2005) Evaluation of the suitability of six host genes as internal control in real-time RT-PCR assays in chicken embryo cell cultures infected with infectious bursal disease virus. Vet Microbiol 110(3–4):155–165.

    Article  CAS  Google Scholar 

  95. Allain CC, Poon LS, Chan CS, Richmond W, Fu PC (1974) Enzymatic determination of total serum cholesterol. Clin Chem 20(4):470–475.

    Article  CAS  Google Scholar 

  96. Surai P, Fisinin V (2014) Selenium in poultry breeder nutrition: An update. Anim Feed Sci Technol 191:1–15.

    Article  CAS  Google Scholar 

  97. Tufarelli V, Casalino E, D’Alessandro AG, Laudadio V (2017) Dietary phenolic compounds: biochemistry, metabolism and significance in animal and human health. Curr Drug Metab 18(10):905–913.

    CAS  PubMed  Google Scholar 

  98. Tag-el-Din SEI (1999) Fixed vs. Variable Return Models: An Economic Analysis. 8th Intensive Orientation Seminar on ÔIslamic Economics, Banking and Finance, Islamic, Leicester, pp 23–27.

    Google Scholar 

  99. Tabib MA, Karim M, Haque M, Khaliq Q, Solaiman A (2014) Effect of planting arrangements on productivity of cotton + mungbean intercropping systems. Bangladesh Agronomy Journal 17(1):11–22.

    Article  Google Scholar 

  100. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2 – ∆∆CT method. Methods 25(4):402–408.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Not applicable.

Funding

This research received no external funding.

Author information

Authors and Affiliations

  1. Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box:24885, 13109, Safat, Kuwait

    Hanan S. Al-Khalaifah

  2. Department of Animal Wealth Development, Veterinary Economics and Farm Management, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt

    Sara. E Shahin

  3. Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt

    Anaam E. Omar & Doaa Ibrahim

  4. Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt

    Haiam A. Mohammed

  5. Department of Animal Wealth Development, Biostatistics, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt

    Hala. I Mahmoud

Authors
  1. Hanan S. Al-Khalaifah
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sara. E Shahin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anaam E. Omar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Haiam A. Mohammed
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hala. I Mahmoud
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Doaa Ibrahim
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All authors have read and approved the manuscript. Conceptualization, D.I, S.E.S, A.E.O, H.S.A, H.A.I and H.A.M.; methodology, D.I, S.E.S, A.E.O, H.S.A, H.A.I and H.A.M., software, D.I, S.E.S, H.A.M.; validation, D.I, S.E.S and A.E.O.; formal analysis, D.I, H.A.I and H.A.M.; investigation, .I, S.E.S, A.E.O, H.S.A, H.A.I and H.A.M.; resources, .I, S.E.S, A.E.O, H.S.A, H.A.I and H.A.M.; data curation, X.X.; writing—original draft preparation, D.I, S.E.S, A.E.O, H.S.A, H.A.I and H.A.M., writing—review and editing, D.I, S.E.S, A.E.O, H.S.A, and H.A.M visualization, D.I, S.E.S, A.E.O, H.S.A, H.A.I and H.A.M.; supervision, D.I, A.E.O, H.S.A, and H.A.M; funding acquisition, D.I, S.E.S, A.E.O, H.S.A, H.A.I and H.A.M. The authors have read and approved the manuscript.

Corresponding authors

Correspondence to Hanan S. Al-Khalaifah or Doaa Ibrahim.

Ethics declarations

Ethics approval and consent to participate

All animal protocols and arrival guidelines were approved by the Institutional Animal Care and Use Committee (ZU-IACUC/3/M/2019), Poultry Research Unit, Faculty of Veterinary Medicine, Zagazig University.

Consent for publication

Not applicable.

Availability of data and materials

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Al-Khalaifah, H.S., Shahin, S.E., Omar, A.E. et al. Effects of graded levels of microbial fermented or enzymatically treated dried brewer’s grains on growth, digestive and nutrient transporter genes expression and cost effectiveness in broiler chickens. BMC Vet Res 16, 424 (2020). https://doi.org/10.1186/s12917-020-02603-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s12917-020-02603-0

Keywords

  • Fermented dried brewer’s grain
  • Gene expression
  • Digestion gene
  • Nutrient transporter
  • Profitability

BMC Veterinary Research

ISSN: 1746-6148

Contact us