Table 8 Primers Sequences and target genes used for quantitative real-time PCR
Genesa | Gene full name | Primer sequence (5′-3′) | Accession No. |
|---|---|---|---|
PGA5 | Pepsinogen A | F-TCCGTCTACCTGAGCAAGGAT R- AAGCAGGCGACGTACTTGTT | NM_204878.1 |
PGC | Pepsinogen C | F-ATCGGGATTGAGGA↓CTTCGC R- TGAAGACCTGGTTGGGAACG | NM_204877.2 |
AMY2A | Pancreatic alpha 2A amylase | F-CGGAGTG↓GATGTTAACGACTGG R-ATGTTCGCAGACCCAGTCATTG | NM_001001473.2 |
PNLIP | Pancreatic lipase | F-GCATCTGGGAAG↓GAACTAGGG R- TGAACCACAAGCATAGCCCA | NM_001277382.1 |
CCK | Cholecystokinin | F-AGGTTCCACTGGGAGGTTCT R-CGCCTGCTGTTCTTTAGGAG | XM_015281332.1 |
CELA1 | Chymotrypsin-like elastase family, member 1 | F-AGCGTAAGGAAATGGGGTGG R-GTGGAGACCCCATGCAAGTC | XM_015300368.1 |
GLUT1 | Glucose transporter-1 (SLC2A1) | F-TCCTCCTGATCAACCGCAAT R-TGTGCCCCGGAGCTTCT | NM_205209.1 |
GLUT2 | Glucose transporter-2 (SLC2A2) | F-TGATCGTGGCACTGATGGTT R-CCACCAGGAAGAC↓GGAGATA | NM_207178.1 |
CAT1 | Cationic amino acid transporter-1 (SLC7A1) | F-CAAGAGGAAAACTCCAGTAATTGCA R- AAGTCGAAGAGGAAGGCCATAA | XM_015277945.1 |
CAT2 | Cationic amino acid transporter-2 (SLC7A2) | F-TGCTCGCGTTCCCAAGA R- GGCCCACAGTTCACCAACAG | XM_015285435.1 |
LAT1 | L-type amino acid transporter-1 (SLC7A7) | F-GATTGCAACGGGTGATGTGA R- CCCCACACCCACTTTTGTTT | KT876067.1 |
PepT1 | Peptide transporter-1 (SLC15A1) | F-TACGCATACTGTCACCATCA R-TCCTGAGAACGGACTGTAAT | AY029615.1 |
PepT2 | Peptide transporter-2 (SLC15A2) | F-TGACTGGGCATCGGAACAA R-ACCCGTGTCACCATTTTAACCT | NM_001319028.1 |
GAPDH | Glyceraldahyde -3-phosphate dehydrogenase | F-GGTGGTGCTAAGCGTGTTA R-CCCTCCACAATGCCAA | NM205518 |