- Research article
- Open Access
Phenotyping and comparing the immune cell populations of free-ranging Atlantic bottlenose dolphins (Tursiops truncatus) and dolphins under human care
BMC Veterinary Research volume 13, Article number: 78 (2017)
Studies suggest that free-ranging bottlenose dolphins exhibit a suppressed immune system because of exposure to contaminants or microorganisms. However, due to a lack of commercially available antibodies specific to marine mammal immune cell surface markers, the research has been indecisive. The purpose of this study was to identify cross-reactive terrestrial-specific antibodies in order to assess the changes in the immune cell populations of dolphins under human care and free-ranging dolphins. The blood and PBMC fraction of blood samples from human care and free-ranging dolphins were characterized by H&E staining of cytospin slides and flow cytometry using a panel of terrestrial-specific antibodies.
In this study, we show that out of 65 terrestrial-specific antibodies tested, 11 were cross-reactive and identified dolphin immune cell populations within their peripheral blood. Using these antibodies, we found significant differences in the absolute number of cells expressing specific markers within their lymphocyte and monocyte fractions. Interestingly, the peripheral blood mononuclear cell profile of free-ranging dolphins retained an additional population of cells that divided them into two groups showing a low (<27%) or high (>56%) percentage of smaller cells resembling granulocytes.
We found that the cross-reactive antibodies not only identified specific changes in the immune cells of free-ranging dolphins, but also opened the possibility to investigate the causal relationship between immunosuppression and mortality seen in free-ranging dolphins.
In recent years there has been a drastic increase in the number of cetacean strandings and mortalities along the coast of the United States, which the National Marine Fisheries Service (NMFS) has termed unusual mortality events (UMEs). UMEs have been occurring yearly amongst a variety of marine species in different areas of the U.S. However, one of the largest UMEs which began in July 2013 has affected hundreds of bottlenose dolphins along the Atlantic coast of the U.S. . Although it is often very difficult to determine the cause of an UME, NMFS attributes many to biotoxins, ecological factors, infectious diseases, such as morbillivirus , or human interactions . Interestingly, studies have shown that high trophic level predators, such as dolphins, have very high concentrations of brevetoxin  and chemical pollutants  in their tissues as a result of consuming lower trophic level fish that also have high concentrations of these contaminants.
Animal and human studies have demonstrated the importance of the immune system for combating infectious diseases and cancer. Indeed, number of reports suggest a correlation between environmental contaminants, immunosuppression, and diseases susceptibility in marine mammals. For example, several studies have found changes in immunological parameters, such as absolute number of eosinophils and lymphocytes  and lymphocyte proliferation in dolphins exposed to certain contaminants [7–9] that may adversely lead to cell anergy or autoimmune diseases [7, 8]. Other studies showed an inverse correlation between contaminant levels and immune cells and their function in marine mammals, such as absolute number of lymphocytes, eosinophils, and monocytes , lymphocyte proliferation [10–13], phagocytosis , and nonspecific  and specific  immune responses. The fact that the incidence of tumors is also increasing in free-ranging dolphins suggests that tumors can evade immune surveillance due to changes in their immune parameters [17, 18].
In addition to environmental contaminants, the immunosuppressive effects of infectious diseases in dolphins have also been reported in several studies. For example, dolphins infected with lobomycosis, a mycotic skin disease, displayed a suppressed immune system compared to dolphins without a visible infection . Dolphins with antibody titers positive to bacterial infection, Chlamydiaceae, showed changes in their innate and adaptive immunological parameters . Dolphins with positive morbilliviral antibody titers had decreased T cell proliferation and absolute number, suggestive of adverse changes in their immune system . Also, the deceased dolphins affected by UMEs on the Atlantic coast of the U.S. and the Gulf of Mexico had high prevalence of morbilliviral antigens, lesions indicative of a morbillivirus infection, and secondary infections . Of note, prior to these studies, it was reported that dolphins affected by an UME in the Gulf of Mexico had high levels of numerous immunosuppressive chemicals and toxins in their liver and opportunistic infections .
Overall, these studies [6–16, 19–21, 24] shed light on the immunosuppressive effects of contaminants or microorganisms on dolphins’ immune system. In order to better investigate a cause and effect relationship between contaminants, immunosuppression, diseases susceptibility and mortality leading to UMEs, it is essential to characterize, monitor, and evaluate specific changes in their immune cells. Flow cytometry is commonly used method to monitor the immune status and disease progression in humans and experimental laboratory animals. However, this method has not been utilized in dolphins due to lack of commercially available antibodies specific to marine mammal immune cell surface markers. We addressed this limitation by identifying cross-reactive terrestrial-specific antibodies to phenotype the immune cells of dolphins under human care. We then utilized these selected antibodies to monitor the immune status of free-ranging dolphins by comparing their immune cell subsets to those of dolphins under human care.
Staining media and antibodies
Media containing PBS 1X, 2% heat-inactivated FCS and 2 mM EDTA was used to label cells with monoclonal antibodies. Terrestrial monoclonal antibodies were purchased from BD Bioscience (San Jose, CA) and eBioscience (San Diego, CA). Antibodies tested that were not cross-reactive; Human-specific: CD1a (HI149), CD19 (SJ25C1), CD19 (1D3), CD62L (DREG56), HLA-DR (G46-6 or L243) (eBioscience, CA), CD3 (UCHT1), CD3 (SP34-2), CD3e (SP34), CD3e (APA1/1), CD4 (M-T477), CD4 (L200), CD8 (SK1), CD8 (HIT8a), CD8 (RPA-T8), CD11b (ICRF44), CD15 (HI98), CD19 (HIB19), CD25 (MA251), CD45 (HI30), Ig Kappa Light Chain (polyclonal), HLA-ABC (G46-2.6), and HLA-ABC (DX17) (BD Bioscience, CA). Mouse-specific: CD4 (GK1.5), CD8a (53-6.7), CD11b (M1/70), IA-IE (M5/114.15.2) (eBioscience, CA), CD3 complex (17A2), CD3e (145-2C11), CD3e (500A2), CD4 (RM4-5), CD45 (30-F11), and CD49b (DX5) (BD Bioscience, CA). Rat-specific: CD80 (3H5) (BD Bioscience, CA).
Antibodies tested that were cross-reactive but not selected due to low detectability of corresponding markers; Human-specific: CD11c (3.9) (eBioscience, CA). Mouse-specific: CD4 (H129.19), CD4 (RM4-4), CD8b (H35-17.2), CD40 (3/23), CD80 (16-10A1), Ly-6G (1A8), H-2Kb (AF6-88.5) (BD Bioscience), CD11c (N418), CD25 (PC61.5), CD62L (MEL-14), B220 (RA3-6B2), and H2Ld-Db (28-14-8) (eBioscience, CA). Rat-specific: CD3 (G4.18), CD4 (OX-35), CD8a (OX-8), CD86 (24 F), and RT1A (OX-18) (BD Bioscience, CA). Pig-specific: CD3e (BB23-8E6-8C8), CD4a (74-12-4), and CD8a (76-2-11) (BD Bioscience, CA).
Antibodies tested that were cross-reactive and selected due to high detectability of corresponding markers; Human-specific: CD4 (RPA-T4), CD14 (61D3), CD56 (MEM188), HLA-DR (LN3) (eBioscience, CA), CD8 (G42-8), Ig Lambda Light Chain (polyclonal) (BD Bioscience, CA), and HLA-ABC (W6/32) (DAKO). Mouse-specific: CD40 (HM40-3) and Ly-6G and Ly-6C (RB6-8C) (BD Bioscience, CA). Rat-specific: CD3 (1F4) and CD11b (WT.5) (BD Bioscience).
Isotype controls for selected cross-reactive antibodies: IgM kappa (G155-228), IgG2a kappa (G155-178), IgA kappa (M18-254), IgM lambda (G235-1), IgG2b (A95-1) (BD Bioscience, CA), and IgG1 kappa (P18.104.22.168.1), IgG2a kappa (eBM2a), and IgG2b kappa (eBMG2b) (eBioscience, CA). Isotype controls for clone HM40-3 and Ig Lambda Light Chain were not available.
Source of dolphin blood samples
Atlantic bottlenose dolphin (Tursiops truncatus) blood samples were obtained from nine captive dolphins under human care at the Georgia Aquarium in Atlanta, Georgia and 16 free-ranging dolphins in the estuarine areas of Charleston, South Carolina as part of the Dolphin Health and Environmental Risk Assessment (HERA) Project. Techniques and protocols related to the dolphin capture-release practices of the HERA Project have been previously described . All samples were shipped overnight and processed immediately. The Atlantic bottlenose dolphin HERA Project was initiated as a multidisciplinary, integrated, collaborative effort in 2003 to assess individual and population health in two southeast coastal regions of the USA: Charleston, SC and the Indian River Lagoon, FL . All methods used in the HERA Project for capture and sample collection were approved under NMFs Scientific Research Permit Nos. 9981678 and 14352-02 issued to G. Bossart and Florida Atlantic University IACUC Protocol Number A10-13. Samples from animals under human care were obtained from the Georgia Aquarium facilities as part of a routine preventative medicine program and were from individual animals with normal clinical history and physical examination.
Ficoll-Hypaque technique is commonly utilized in humans to isolate PBMCs from peripheral blood to study innate and adaptive immune cells and to use monocytes to generate ex vivo dendritic cells (DCs). We used this method to isolate peripheral blood mononuclear cells (PBMCs) from dolphin peripheral blood by centrifugation of blood samples with Ficoll-Paque PLUS (Amersham Biosciences, NJ) in 50 ml Falcon-tubes for 30 min at 900 × g at 20 °C. The viability of PBMCs was quantitated by trypan blue staining and was >95%. A fraction of the PBMCs were used for labeling with corresponding monoclonal antibodies for further analysis on flow cytometry. Remaining PBMCs were frozen in freezing media containing 90% fetal calf serum (FCS) and 10% dimethyl sulfoxide (DMSO) for further analysis. There were no differences in expression levels of cellular markers observed whether fresh or frozen PBMCs were used for phenotyping.
Immunophenotyping of PBMCs
Flow cytometry is commonly used to simultaneously measure and analyze multiple characteristics of cells within PBMCs, including size, granularity, and surface marker expression. Prior to labelling, fresh or frozen PBMCs were washed three times in a staining media by resuspending and repeating the centrifugation steps at 600 × g for 5 min. For labeling, cells pre-incubated with mouse and rat serum were stained with the fluorochrome-conjugated antibodies or matching isotype control antibodies for 30 min on ice in staining media. To remove any unbound antibodies, the cells were washed two times by centrifugation at 600 × g for 5 min at 4 °C. Finally, the cells were transferred to flow cytometry sample storage tubes containing staining media and 2% paraformaldehyde and kept at 4 °C before acquisition on FACSCalibur flow cytometer using Cell QuestPro acquisition software (BD Bioscience, San Jose, CA). The data were analyzed using FlowJo software (TreeStar, Ashland, OR).
Blood smear and cytopsin slide preparations
Blood smears were prepared using peripheral blood from dolphins under human care. Cytospin slides were prepared by spinning isolated PBMCs from dolphins under human care and free-ranging dolphins at 800 rpm for 5 min on a cytospin centrifuge (Shandon Inc., Pittsburgh, PA). Blood smear and cytospin slides were fixed and stained with Fisher Scientific three step stain set. Slides were photographed on an Olympus Provis AX microscope using Olympus cellSens software.
Values are presented as means ± SEM. To determine the significance of data, statistical comparisons were performed using a t-test assuming unequal variances. A p value <0.05 was statistically significant and shown as an asterisk (*).
PBMCs isolated from peripheral blood display differences between dolphins under human care and free-ranging dolphins
Similar to humans and other species, dolphin peripheral blood smears showed a heterogeneous population of white bloods cells (WBCs): monocytes, lymphocytes, and granulocytes, including neutrophils, basophils and eosinophils (Fig. 1a). Using Ficoll-Hypaque technique, we isolated on average 1.3 × 106 PBMCs/ml ranging from 0.9 to 1.5 × 106 PBMCs/ml from peripheral blood of dolphins under human care (Table 1). However, the absolute number of PBMCs isolated from peripheral blood of free-ranging dolphins was significantly higher (three-fold) with an average of 3.1 × 106 PBMCs/ml ranging from 1.3 to 9.0 × 106 PBMCs/ml (Table 1). In addition, cytospin slides of isolated PBMCs from free-ranging dolphins showed differences in cellular composition when compared to dolphins under human care, specifically with a significant increase in granulocytes and decrease in monocytes and lymphocytes (Fig. 1b).
Flow cytometry profile of PBMCs display differences between dolphins under human care and free-ranging dolphins
Similar to human PBMCs, the forward-scatter and side-scatter (FSC/SSC) profile of total isolated PBMCs of dolphins under human care showed two regions, resembling the lymphocyte and monocyte fractions (Fig. 2a). Interestingly, within the FSC/SSC profile of free-ranging dolphins, we observed a third region ranging from low (<27%, group A free-ranging dolphins) to high (>56%, group B free-ranging dolphins) percentage of cells with smaller size and similar granularity but not identical density to granulocytes (Fig. 2b and 2c).
Terrestrial-specific antibodies identify immune cell subsets within the PBMCs of dolphins
There are currently no commercially available marine-specific antibodies to distinguish cell types within PBMCs of dolphins. In other species when specific monoclonal antibodies are available lymphocyte fraction (region 1) contains cells that express lymphocyte markers, including T cells (CD3/4/8), B cells (CD19), and NK cells (CD56/49b), while monocyte fractions (region 2) contains cells that express monocyte (CD14/11b) and myeloid markers (CD11b/40), all of them also differentially express major histocompatibility (MHC) antigens (MHC class I/II). In order to identify these immune cells within each region of dolphins’ PBMCs, we took advantage of terrestrial antibodies specific to human, mouse and rat. Amongst the 65 antibodies we tested, 32 showed cross-reactivity of which 11 with the highest cross-reactivity were selected for further identification of these cells within the total PBMCs (Table 2). We found that CD3/4/8, Ig Lambda (λ)/CD40/11b, and CD56/11b monoclonal antibodies were able to distinguish the cells within region 1 as T, B, and NK cells (Fig. 3b) while CD14/11b/Ly-6G&C/40 and CD11b/Ly-6G&C identified monocytes and myeloid cells respectively within region 2 of dolphins under human care (Fig. 3c). In addition, both regions contain cells expressing antigen-presenting molecules, MHC class I and II (Fig. 3b and 3c). These selected monoclonal antibodies were also able to identify the same cell subsets within regions 1 and 2 of normal free-ranging dolphin PBMCs (Fig. 4b and 4c). Interestingly these antibodies detected cells within region 3 of free-ranging dolphins with characteristics of lymphocytes, monocytes, myeloid cells, and granulocytes but lacked expression of antigen-presenting molecules MHC class I and II (Fig. 5b).
Frequency of immune cells within the PBMCs of dolphins under human care and free-ranging dolphins expressing specific surface markers
Our data shows that the differences in FSC/SSC profiles observed between dolphins under human care and free-ranging dolphins reflect increases or decreases in average percentage and absolute number of each immune cell within the lymphocyte, monocyte, and granulocyte regions. Indeed, the average percentage of cells in the lymphocyte region expressing CD4, Ig λ, CD56 and MHC class II surface markers was similar between dolphins under human care and group A and B free-ranging dolphins (Fig. 6a). However the percentage of cells expressing CD3 and CD8 were significantly increased while CD11b was decreased in group A and CD40 was increased in group B free-ranging dolphins when compared to dolphins under human care (Fig. 6a). In addition, we observed a significant increase in percentage of cells expressing MHC class I in group A when compared to group B free-ranging dolphins (Fig. 6a). Then we compared the absolute number of dolphins under human care and free-ranging dolphins’ cells within the lymphocyte region expressing the indicated markers. We found a significant increase in absolute number of group A free-ranging dolphin cells expressing CD3 and CD8 and a decrease in CD11b when compared to dolphins under human care. Finally, group B free-ranging dolphins showed a significant decrease in the absolute number of cells expressing all markers and all markers except CD8 when compared to group A free-ranging dolphins and dolphins under human care, respectively (Fig. 7a). We also compared the differences in the percentage and absolute number of cells within the monocyte region between dolphins under human care and free-ranging dolphins. There was a significant decrease in the percentage of group A free-ranging dolphin cells expressing CD14, CD11b, and MHC class II compared to dolphins under human care (Fig. 6b). There was also a significant decrease in percentage of group B free-ranging dolphin cells expressing MHC class I when compared to group A free-ranging dolphins (Fig. 6b). When the absolute number of cells within the monocyte region was compared, we found a significant decrease in the absolute number of group A free-ranging dolphin cells expressing all markers, except Ly-6G&C when compared to dolphins under human care (Fig. 7b). In addition, we found group B free-ranging dolphins had a significant decrease in absolute number of cells expressing all markers when compared to dolphins under human care and group A free-ranging dolphins (Fig. 7b). Finally, we compared the average percentage and absolute number of cells expressing each indicated marker within the granulocyte region only seen in group A and B free-ranging dolphins. We found that group B with a high increase in granulocytes on FSC/SSC plots showed a greater percentage of cells for almost all surface markers, although not significant (Fig. 6c). Also, the absolute number of cells expressing each indicated marker was significantly increased in group B compared to group A free-ranging dolphins except CD56, MHC class I, and MHC class II (Fig. 7c).
In this study, we investigated dolphins under human care that served as a healthy control model for dolphins subject to routine health care in order to characterize their immune cell subsets and compare them side by side to those of free-ranging dolphins. Using a hemocytometer, we found that Atlantic bottlenose dolphins under human care have on average 6.5 × 106 white blood cells (WBCs)/ml of blood (Table 3) which is lower than 10.95 × 106 WBCs/ml reported in previous studies using an automatic cell counter [27, 28]. This discrepancy is most likely due to differences in counting methods. Indeed, using an automatic cell counter, others [25, 29] and we (Table 3) found that free-ranging Atlantic bottlenose dolphins have 11.0 × 106 WBCs/ml of blood and 12.7 × 106 WBCs/ml, respectively. Within the WBCs, studies showed that dolphins under human care have 2.3% monocytes, 21.7% lymphocytes, 60.3% neutrophils, <1% basophils, and 14.5% eosinophils [27, 28, 30, 31], while free-ranging dolphins have 2.9% monocytes, 21.1% lymphocytes, 40.3% neutrophils, <1% basophils, and 35.9% eosinophils [25, 29]. We observed that dolphins under human care have 3.9% monocytes, 17.8% lymphocytes, 63.3% neutrophils, 1.2% basophils, and 12.1% eosinophils, while free-ranging dolphins have 2.7% monocytes, 17% lymphocytes, 39.5% neutrophils, 0.9% basophils, and 39.6% eosinophils within their WBCs (Table 3). The decrease in lymphocytes and neutrophils and increase in eosinophils that we observed in free-ranging compared to dolphins under human care is consistent with the WBC differentials reported in the previous studies [25, 27–31]. Of note, dolphins under human care and free-ranging dolphins have a leukocyte differential similar to humans, except their eosinophil count which is much higher than the 1–4% range seen in humans.
The FSC/SSC flow cytometery profile of free-ranging dolphins’ PBMCs revealed that they can be divided into group A and B based on low (<27%) and high (>56%) percentage of granulocytes respectively (Fig. 2b and 2c). In addition, group B free-ranging dolphins have the highest average PBMC count of 3.5 × 106 per ml compared to 2.9 × 106 per ml for group A and 1.3 × 106 per ml for dolphins under human care (Table 1). Furthermore, the PBMCs of group B free-ranging dolphins have on average a differential of 63% granulocytes, 8% monocytes, and 16% lymphocytes while group A has 13% granulocytes, 13% monocytes, and 56% lymphocytes, and dolphins under human care have 17% monocytes and 59% lymphocytes (Table 1). Therefore, our data revealed that group B free-ranging dolphins have the lowest lymphocyte and monocyte fractions of PBMCs compared to dolphins under human care and group A free-ranging dolphins. Consistent with our flow data, cytospin slides prepared from PBMCs of group B free-ranging dolphins also showed a decrease in lymphocytes and monocytes and an increase in granulocytes compared to dolphins under human care (Fig. 1b). Noteworthy, these observed changes between dolphins under human care, group A, and group B free-ranging dolphins were not unique to their PBMCs since we found similar changes in total WBC count and percentage of lymphocytes, monocytes, and granulocytes within their WBCs. Indeed, group B compared to group A free-ranging dolphins on average have an increase in total WBC count 13.7 × 106 vs. 12.4 × 106 per ml and granulocytes 83.8% vs. 78.8% and a decrease in lymphocytes 12.8% vs. 18.5% and monocytes 2.5% vs. 2.8% (Table 3). Interestingly, within the granulocyte fraction of total WBCs, group B free-ranging dolphins also have an increase in neutrophils 51.8% vs. 35.4% and decrease in eosinophils 31.3% vs. 42.4% compared to group A (Table 3).
Previous studies have labelled cells within various dolphin tissues using human-specific antibodies [32, 33], which suggest that human and dolphin cells share epitopes on their cell surface markers. Our data further suggests that cell surface markers on dolphin immune cells share epitopes with humans and other species, since three of three pig, seven of eight rat, 14 of 25 mouse and eight of 29 human specific antibodies were cross-reactive. Interestingly, out of the 11 antibodies we selected to label dolphin immune cells, seven human, two mouse, and two rat-specific antibodies had the highest cross-reactivity. Therefore, our data demonstrated that using pan surface markers commonly used in mouse and human studies, we could detect dolphin immune cell subsets, including CD3 T cells, CD56 NK cells, and CD14/CD11b monocytes (Fig. 3 and 4). However, we were not able to detect B cells in dolphins using the monoclonal antibody specific to pan B cell marker CD19. Thus, we instead utilized Ig lambda (λ) and CD40, as well as CD11b known also to detect B cell subsets (Fig. 3b and 4b). De Guise et al., , reported that 14.1% and 22.63% of Atlantic bottlenose dolphins’ PBMCs expressed CD19 and CD21, respectively using non-commercially available antibodies. However, these antibodies were not available to us for comparison. Altogether, our selected antibodies were able to detect dolphin immune cell subsets, even though they may not have optimally represented the true percentage of immune cells and expression of their markers within dolphin peripheral blood.
In humans and rodents, MHC class II expression is restricted to antigen-presenting cells. We observed that nearly all dolphin B and T lymphocytes and monocytes express MHC class II (Fig. 3 and 4). Our finding is consistent with previous studies that showed more than 90% of bottlenose dolphins’  and beluga whales’  peripheral blood cells within lymphocyte region express MHC class II, which is also observed in ungulates and carnivores such as swine , equine , canine , and feline . Although, MHC class I is displayed on all human and mouse nucleated cells, we found that only a small percentage of dolphin cells expressed MHC class I (Fig. 3 and 4). Furthermore, our data revealed that a similar percentage of cells expressed β2-microglobulin, an invariant chain associated with MHC class I alpha chain (data not shown). Using bovine-specific antibodies, De Guise et al. , reported that majority of the cells within the lymphocyte region of beluga whales expressed MHC class I. Whether the discrepancies in MHC class I expression in these studies was due to the antibodies used for labelling or represents their true expression patterns needs further investigation.
The WBC profile of dolphins contained two populations of small and large cells with similar granularity within granulocyte region (data not shown). During PBMC isolation, Ficoll removed nearly all of the large but not small granulocyte-like cells from blood samples of dolphins. Interestingly however, the percentage of low density small granulocyte-like cells retained within PBMCs of free-ranging dolphins, which can also be seen on the cytospin slides (Fig. 1b) was significantly higher than those within PBMCs of dolphins under human care (Fig. 2). In addition, these small granulocyte-like cells expressed all of the markers, except MHC class I and II (Fig. 5b). Interestingly, we observed that group B free-ranging dolphins with the highest percentage of granulocytes in their PBMCs were classified as diseased animals (Table 3). Whether the observed changes in percentage, absolute number, and surface markers of cells within PBMCs of dolphins under human care and group A and B free-ranging dolphins was due to increase in precursor cells, differences in maturational stages of cells or dolphins’ health status merits further evaluation.
Alterations in WBC count and distribution could provide insight into the health status of dolphins, Indeed, It was found that a bottlenose dolphin under human care infected with Aspergillus fumigatus had a higher than normal WBC count which decreased after treatment . Another study found that seals fed fish contaminated with immunosuppressive organochlorines had an increase in granulocytes which the authors stated may indicate bacterial infections . However, alterations in WBC count offers little information on specific changes imposed by environmental factors on immune cell subsets and their function. In this study, we have identified cross-reactive terrestrial-specific antibodies that allowed us to characterize the immune cell subsets of dolphins under human care and free-ranging dolphins. Our findings opened the possibility of utilizing flow cytometry for routine health assessment by monitoring specific changes in immune cells of dolphins caused by environmental contaminants or infectious agents with the goal of understanding the pathogenesis of diseases.
Fluorescence activated cell sorting
Forward scatter/side scatter
Hematoxylin and eosin
Health and Environmental Risk Assessment
Institutional Animal Care Unit Committee
Major histocompatibility complex
Natural killer cells
National Marine Fisheries Service
Peripheral blood mononuclear cells
Unusual mortality events
White bloods cells
2013–2014 Bottlenose dolphin unusual mortality event in the Mid-Atlantic [http://www.nmfs.noaa.gov/pr/health/mmume/midatldolphins2013.html]
2013–2014 Mid-Atlantic bottlenose dolphin UME investigation results [http://www.nmfs.noaa.gov/pr/health/mmume/midatldolphins2013_results.htm]
NOAA. Marine mammal unusual mortality events 1991–2013. Number of declared events per year, by cause. 2013.
Flewelling LJ, Naar JP, Abbott JP, Baden DG, Barros NB, Bossart GD, Bottein MY, Hammond DG, Haubold EM, Heil CA, et al. Brevetoxicosis: red tides and marine mammal mortalities. Nature. 2005;435(7043):755–6.
Fair PA, Adams J, Mitchum G, Hulsey TC, Reif JS, Houde M, Muir D, Wirth E, Wetzel D, Zolman E, et al. Contaminant blubber burdens in Atlantic bottlenose dolphins (Tursiops truncatus) from two southeastern US estuarine areas: concentrations and patterns of PCBs, pesticides, PBDEs, PFCs, and PAHs. Sci Total Environ. 2010;408(7):1577–97.
Schaefer AM, Stavros HC, Bossart GD, Fair PA, Goldstein JD, Reif JS. Associations between mercury and hepatic, renal, endocrine, and hematological parameters in Atlantic bottlenose dolphins (Tursiops truncatus) along the eastern coast of Florida and South Carolina. Arch Environ Contam Toxicol. 2011;61(4):688–95.
Mori C, Morsey B, Levin M, Nambiar PR, De Guise S. Immunomodulatory effects of in vitro exposure to organochlorines on T-cell proliferation in marine mammals and mice. J Toxicol Environ Health A. 2006;69(3–4):283–302.
Mori C, Morsey B, Levin M, Gorton TS, De Guise S. Effects of organochlorines, individually and in mixtures, on B-cell proliferation in marine mammals and mice. J Toxicol Environ Health A. 2008;71(4):266–75.
Fair PA, Romano T, Schaefer AM, Reif JS, Bossart GD, Houde M, Muir D, Adams J, Rice C, Hulsey TC, et al. Associations between perfluoroalkyl compounds and immune and clinical chemistry parameters in highly exposed bottlenose dolphins (Tursiops truncatus). Environ Toxicol Chem. 2013;32(4):736–46.
Lahvis GP, Wells RS, Kuehl DW, Stewart JL, Rhinehart HL, Via CS. Decreased lymphocyte responses in free-ranging bottlenose dolphins (Tursiops truncatus) are associated with increased concentrations of PCBs and DDT in peripheral blood. Environ Health Perspect. 1995;103 Suppl 4:67–72.
Schwacke LH, Twiner MJ, De Guise S, Balmer BC, Wells RS, Townsend FI, Rotstein DC, Varela RA, Hansen LJ, Zolman ES, et al. Eosinophilia and biotoxin exposure in bottlenose dolphins (Tursiops truncatus) from a coastal area impacted by repeated mortality events. Environ Res. 2010;110(6):548–55.
Nakata H, Sakakibara A, Kanoh M, Kudo S, Watanabe H, Nagai N, Miyazaki N, Asano Y, Tanabe S. Evaluation of mitogen-induced responses in marine mammal and human lymphocytes by in-vitro exposure of butyltins and non-ortho coplanar PCBs. Environ Pollut. 2002;120(2):245–53.
De Swart RL, Ross PS, Vedder LJ, Timmerman HH, Heisterkamp S, Loveren HV, Vos JG, Reijnders PJ, Osterhaus AD. Impairment of immune function in harbor seals (Phoca vitulina) feeding on fish from polluted waters. Ambio. 1994;23(2):155–9.
Levin M, Morsey B, Mori C, Guise S. Specific non-coplanar PCB-mediated modulation of bottlenose dolphin and beluga whale phagocytosis upon in vitro exposure. J Toxicol Environ Health A. 2004;67(19):1517–35.
De Swart RL, Ross PS, Timmerman HH, Vos HW, Reijnders PJ, Vos JG, Osterhaus AD. Impaired cellular immune response in harbour seals (Phoca vitulina) feeding on environmentally contaminated herring. Clin Exp Immunol. 1995;101(3):480–6.
Ross PS, De Swart RL, Reijnders PJ, Loveren HV, Vos JG, Osterhaus AD. Contaminant-related suppression of delayed-type hypersensitivity and antibody responses in Harbor seals fed herring from the Baltic Sea. Environ Health Perspect. 1995;103:162–7.
Bossart GD, Ghim S, Rehtanz M, Goldstein JD, Varela RA, Ewing RY, Fair PA, Lenzi R, Joseph B, Hicks CL, et al. Orogenital neoplasia in Atlantic bottlenose dolphins (Tursiops truncatus). Aquat Mamm. 2005;31(4):473–80.
Bossart GD, Romano TA, Peden-Adams MM, Rice CD, Fair PA, Goldstein JD, Kilpatrick D, Cammen K, Reif JS. Hematologoical, biochemical, and immunological findings in Atlantic bottlenose dolphins (Tursiops truncatus) with orogenital papillomas. Aquat Mamm. 2008;34(2):166–77.
Reif JS, Peden-Adams MM, Romano TA, Rice CD, Fair PA, Bossart GD. Immune dysfunction in Atlantic bottlenose dolphins (Tursiops truncatus) with lobomycosis. Med Mycol. 2009;47(2):125–35.
Bossart GD, Romano TA, Peden-Adams MM, Schaefer A, McCulloch S, Goldstein JD, Rice CD, Fair PA, Cray C, Reif JS. Clinicoimmunopathologic findings in Atlantic bottlenose dolphins Tursiops truncatus with positive Chlamydiaceae antibody titers. Dis Aquat Organ. 2014;108(1):71–81.
Bossart GD, Romano TA, Peden-Adams MM, Schaefer A, McCulloch S, Goldstein JD, Rice CD, Saliki JT, Fair PA, Reif JS. Clinicoimmunopathologic findings in Atlantic bottlenose dolphins Tursiops truncatus with positive cetacean morbillivirus antibody titers. Dis Aquat Organ. 2011;97(2):103–12.
Lipscomb TP, Kennedy S, Moffett D, Krafft A, Klaunberg BA, Lichy JH, Regan GT, Worthy GA, Taubenberger JK. Morbilliviral epizootic in bottlenose dolphins of the Gulf of Mexico. J Vet Diagn Invest. 1996;8(3):283–90.
Kuehl DW, Haebler R. Organochlorine, organobromine, metal, and selenium residues in bottlenose dolphins (Tursiops truncatus) collected during an unusual mortality event in the Gulf of Mexico, 1990. Arch Environ Contam Toxicol. 1995;28(4):494–9.
Heaney J, Barrett T, Cosby SL. Inhibition of in vitro leukocyte proliferation by morbilliviruses. J Virol. 2002;76(7):3579–84.
Fair PA, Hulsey TC, Varela RA, Goldstein JD, Adams J, Zolman ES, Bossart GD. Hematology, serum chemistry, and cytology findings from apparently healthy Atlantic bottlenose dolphins (Tursiops truncatus) inhabiting the estuarine waters of Charleston, South Carolina. Aquat Mamm. 2006;32(2):182–95.
Bossart GD, Goldstein JD, Murdoch EM, Fair PA, McCulloch S. Health assessment of bottlenose dolphins in the Indian River Lagoon, Florida and Charleston, South Carolina. Ft. Pierce: Harbor Branch Oceanographic Technical Report No. 93; 2006.
Engelhardt FR. Haematology and plasma chemistry of captive pinnipeds and cetaceans. Aquat Mamm. 1979; 7:11–20.
MacNeill AC. Blood values for some captive cetaceans. Can Vet Jour. 1975;16(7):187–93.
Goldstein JD, Reese E, Reif JS, Varela RA, McCulloch SD, Defran RH, Fair PA, Bossart GD, Hansen L. Hematologic, biochemical, and cytologic findings from apparently healthy Atlantic bottlenose dolphins (Tursiops truncatus) inhabiting the Indian River Lagoon, Florida, USA. J Wildl Dis. 2006;42(2):447–54.
Medway W, Geraci JR. Hematology of the bottlenose dolphin (Tursiops Truncatus). Am J Physiol. 1964;207:1367–70.
Ridgway SH, Simpson JG, Patton GS, Gilmartin BS. Hematologic findings in certain small cetaceans. JAVMA. 1970;157(5):566–75.
Kumar D, Cowan DF. Cross-reactivity of antibodies to human antigens with tissues of the bottlenose dolphin, Tursiops truncatus, using immunoperoxidase techniques. Mar Mamm Sci. 1994;10(2):188–94.
Jaber JR, Fernandez A, Herraez P, Espinosa de los Monteros A, Ramirez GA, Garcia PM, Fernandez T, Arbelo M, Perez J. Cross-reactivity of human and bovine antibodies in striped dolphin paraffin wax-embedded tissues. Vet Immunol Immunopathol. 2003;96(1-2):65–72.
De Guise S, Erickson K, Blanchard M, DiMolfetto L, Lepper HD, Wang J, Stott JL, Ferrick DA. Monoclonal antibodies to lymphocyte surface antigens for cetacean homologues to CD2, CD19 and CD21. Vet Immunol Immunopathol. 2002;84:209–21.
Romano TA, Ridgway SH, Quaranta V. MHC class II molecules and immunoglobulins on peripheral blood lymphocytes of the bottlenosed dolphin, Tursiops truncatus. J Exp Zool. 1992;263(1):96–104.
De Guise S, Bernier J, Martineau D, Beland P, Fournier M. Phenotyping of beluga whale blood lymphocytes using monoclonal antibodies. Dev Comp Immunol. 1997;21(5):425–33.
Crepaldi T, Crump A, Newman M, Ferrone S, Antczak DF. Equine T lymphocytes express MHC class II antigens. J Immunogenet. 1986;13(4):349–60.
Doveren RF, Buurman WA, Schutte B, Groenewegen G, van der Linden CJ. Class II antigens on canine T lymphocytes. Tissue Antigens. 1985;25(5):255–65.
Rideout BA, Moore PF, Pedersen NC. Distribution of MHC class II antigens in feline tissues and peripheral blood. Tissue Antigens. 1990;36(5):221–7.
Reidarson TH, Harrell JH, Rinaldi MG, McBain J. Bronchoscopic and serologic diagnosis of Aspergillus fumigatus pulmonary infection in a bottlenose dolphin (Tursiops truncatus). J Zoo Wildl Med. 1998;29(4):451–5.
The authors thank the entire Health and Environmental Risk Assessment Project staff and Georgia Aquarium veterinary and husbandry staff for their efforts in this project. We thank Lynda Leppert (Georgia Aquarium) and Patricia Fair (Medical University of South Carolina) for providing the WBC counts of the dolphins under human care and free-ranging dolphins, Elisabeth Guinet (Florida Atlantic University), and Erika Nourishirazi (University of Miami Miller School of Medicine) for technical support and critical reading of the manuscript. Also, we thank BD Bioscience for generously donating monoclonal antibodies for testing.
This work was supported by the Georgia Aquarium Research Institute (Atlanta, GA).
Availability of data and materials
The datasets supporting the findings can be found in the tables and figures of the manuscript.
BB, and MZ carried out the processing of the blood samples, their phenotyping and flow cytometry analyses. ST carried out the analyses of isotype controls staining. GB provided blood samples and complete blood cell counts. MNS: Designed and supervised the study, trained Postdoc and master’s students to conduct the experiments. BB and MNS wrote the manuscript. All authors have read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
All methods used in the HERA Project for capture and sample collection were approved under National Marine Fisheries Service (NMFS) Scientific Research Permit Nos. 9981678 and 14352-02 issued to G. Bossart and Florida Atlantic University IACUC Protocol Number A10-13.
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Nouri-Shirazi, M., Bible, B.F., Zeng, M. et al. Phenotyping and comparing the immune cell populations of free-ranging Atlantic bottlenose dolphins (Tursiops truncatus) and dolphins under human care. BMC Vet Res 13, 78 (2017) doi:10.1186/s12917-017-0998-3
- Bottlenose dolphin
- Flow cytometry
- Marine mammal
- Peripheral blood cells