Phenotyping and comparing the immune cell populations of free-ranging Atlantic bottlenose dolphins (Tursiops truncatus) and dolphins under human care

Table 2 Antibodies tested on dolphin Peripheral Blood Mononuclear Cells (PBMCs)

Antibody	Clone	Species	Antibody	Clone	Species
Not cross-reactive			Cross-reactive, not selected
CD1a	HI149	Human^a	CD3	G4.18	Rat^b
CD3	17A2	Mouse^b	CD3e	BB23-8E6-8C8	Pig^b
CD3	UCHT1	Human^b	CD4	H129.19	Mouse^b
CD3	SP34-2	Human^b	CD4	RM4-4	Mouse^b
CD3e	SP34	Human^b	CD4	OX-35	Rat^b
CD3e	APA1/1	Human^b	CD4a	74-12-4	Pig^b
CD3e	145-2C11	Mouse^b	CD8a	OX-8	Rat^b
CD3e	500A2	Mouse^b	CD8a	76-2-11	Pig^b
CD4	M-T477	Human^b	CD8b	H35-17.2	Mouse^b
CD4	L200	Human^b	CD11c	3.9	Human^a
CD4	RM4-5	Mouse^b	CD11c	N418	Mouse^a
CD4	GK1.5	Mouse^a	CD25	PC61.5	Mouse^a
CD8	SK1	Human^b	CD40	23-Mar	Mouse^b
CD8	HIT8a	Human^b	CD62L	MEL-14	Mouse^a
CD8	RPA-T8	Human^b	CD80	16-10A1	Mouse^b
CD8a	53-6.7	Mouse^a	CD86	24F	Rat^b
CD11b	M1/70	Mouse^a	B220	RA3-6B2	Mouse^a
CD11b	ICRF44	Human^b	Ly-6G	1A8	Mouse^b
CD15	HI98	Human^b	MHC I (RT1A)	OX-18	Rat^b
CD19	HIB19	Human^b	MHC I (H-2Kb)	AF6-88.5	Mouse^b
CD19	SJ25C1	Human^a	MHC I (H2Ld-Db)	28-14-8	Mouse^a
CD19	1D3	Mouse^a	Cross-reactive, selected
CD25	MA251	Human^b	CD3	1F4	Rat^b
CD45	HI30	Human^b	CD4	RPA-T4	Human^a
CD45	30-F11	Mouse^b	CD8	G42-8	Human^b
CD49b	DX5	Mouse^b	CD11b	WT.5	Rat^b
CD62L	DREG56	Human^a	CD14	61D3	Human^a
CD80	3H5	Rat^b	CD40	HM40-3	Mouse^b
MHC I (HLA-ABC)	G46-2.6	Human^b	CD56	MEM188	Human^a
MHC I (HLA-ABC)	DX17	Human^b	Ly-6G and Ly-6C	RB6-8C5	Mouse^b
MHC II (HLA-DR)	G46-6 or L243	Human^a	MHC I (HLA-ABC)	W6/32	Human^c
MHC II (IA-IE)	M5/114.15.2	Mouse^a	MHC II (HLA-DR)	LN3	Human^a
Ig κ Light Chain	Polyclonal	Human^b	Ig λ Light Chain	Polyclonal	Human^b

Table lists the terrestrial-specific antibodies that were not cross-reactive, cross-reactive but not selected, or cross-reactive and selected. Letters denote the companies where the antibodies were purchased. ^aeBioscience, ^bBD Bioscience, ^cDAKO

ISSN: 1746-6148