Detection and assessment of electrocution in endangered raptors by infrared thermography
© Melero et al.; licensee BioMed Central Ltd. 2013
Received: 24 September 2012
Accepted: 17 July 2013
Published: 23 July 2013
Most European birds of prey find themselves in a poor state of conservation, with electrocution as one of the most frequent causes of unnatural death. Since early detection of electrocution is difficult, treatment is usually implemented late, which reduces its effectiveness. By considering that electrocution reduces tissue temperature, it may be detectable by thermography, which would allow a more rapid identification. Three individuals from three endangered raptor species [Spanish imperial eagle (Aquila adalberti), Lammergeier (Gypaetus barbatus) and Osprey (Pandion haliaetus)] were studied thermographically from the time they were admitted to a rehabilitation centre to the time their clinical cases were resolved.
The three raptors presented lesions lacking thermal bilateral symmetry and were consistent with electrocution of feet, wings and eyes, visible by thermography before than clinically; lesions were well-defined and showed a lower temperature than the surrounding tissue. Some lesions evolved thermally and clinically until the appearance of normal tissue recovered, while others evolved and became necrotic. A histopathological analysis of a damaged finger amputated off a Lammergeier, and the necropsy and histopathology examination of an osprey, confirmed the electrocution diagnosis.
These results suggest that thermography is effective and useful for the objective and early detection and monitoring of electrocuted birds, and that it may prove especially useful for examining live animals that require no amputation or cannot be subjected to invasive histopathology.
KeywordsThermography Electrocution Raptor Bird of prey Spanish imperial eagle Lammergeier Osprey
Birds of prey have been proposed as good sentinels of environmental changes as they are placed at the top of the food chain and are widespread worldwide . However, 36 (64%) of the 56 species of raptors inhabiting Europe find themselves in an unfavourable state of conservation . Most raptor deaths are caused by direct and indirect actions of humans . One of the most common causes of unnatural death is electrocution as a result of collisions with power lines and the subsequent trauma when the animal falls to the ground [1, 3, 4]. While unnatural mortality can be compensated in a healthy population, it can seriously affect a small population . For example, electrocution poses a significant survival problem for one of the most endangered raptors in the world, the Spanish imperial eagle .
The accuracy of electrocution mortality estimates is limited given the difficulty in determining cause of death . Since determination of electrocution is usually based on anamnesis and clinical signs, i.e., electrical burns , detecting and evaluating an electrocuted bird can prove most difficult since anamnesis is often incomplete, and evidence for electrical trauma may not be immediately detectable . Moreover, an early diagnosis of electrocution is essential for a good prognosis. For these reasons, complementary diagnostic tools, such as histopathology, are used when an amputation or necropsy is required [1, 9]. Nevertheless, histopathology is invasive and may not be feasible or advisable for live animals, especially those that do not require amputation.
Thermography is a non-invasive technique used to assess tissue temperature that can be applied to electrocution detection and assessment. It can be used at a distance of more than 500 m from the animal , reducing its stress and ensuring the safety of the technician. Physiological and pathological thermographic patterns have not yet been established for most species. Nevertheless, reports of thermographic analyses have so far established that the thermal pattern and symmetry of the body are more important than the absolute temperature for diagnosing disease .
Tissue affected by electrocution shock shows reduced vascularisation, innervation, water content  and oxygen saturation . These changes should result in a lower temperature in the affected area, thus raising the possibility that electrocution shock can be detected by thermography. Accordingly, this report applies thermography to detect and assess electrocution to three endangered raptors [14, 15].
Case 1: Spanish imperial eagle (Aquila adalberti)
On 11 September 2008, a juvenile female was admitted to the GREFA wildlife rehabilitation centre in Madrid (Spain). Clinical signs were 7% dehydration, depression, emaciation, arrhythmias, bradycardia and a proximal closed right radial fracture. Electrical lesions were not observed initially, but became visible 3 days later. Treatment included rehydration, antibiotics, a figure‒eight bandage on the right wing and physiotherapy on the right finger IV and the right wing. Finally, the animal was released on 16 March 2009.
Thermal values at different anatomical areas
Differences of temperature (°C)
Within each finger
Equivalent fingers of both feet1
Within each finger
Equivalent fingers of both feet1
Within each finger
Equivalent fingers of both feet1
Right finger IV
Spanish imperial eagle (Case 1)
0.2 - 1.5
Admission2 6.6 Physio.3 5.4
1.9 - 2.6
Left finger IV
Left finger III
Lammergeier (C 2)
Admission2 8.4 Physio.3 3.7
Difference of temperature between legs ( °C)
Osprey (C 3)
Healthy (left wing)
Damaged (right wing)
Temperature (° C)
Middle of the ulna area
Middle of the ulna area
Spanish imperial eagle (C 1)
Removal of the bandage
After the first flight test
Temperature (° C)
Left corneal temperature
Right corneal temperature
Intercorneal temperature difference
Lammergeier (C 2)
Six healthy lammergeiers
29.8 - 32.3 (30.0, 29.8, 31.1, 30.6, 31.2, 32.3)
30.2 - 31.8 (30.2, 30.2, 31.5, 31.0, 31.0, 31.8)
0.2 - 0.5 (0.2, 0.4, 0.4, 0.4, 0.2, 0.5)
After removing the bandage, the thermography of the wings revealed a significantly higher temperature in the right patagium area (7.8°C) than in the left one (4.4°C) with a similar maximum temperature in the middle of both ulna areas (Table 1, Figure 1(4)). This asymmetric thermal pattern was consistent with the patagium retraction caused by periods of immobilisation. Increased vascularity and inflammation in the patagium area led to an increased temperature, which was detected by thermography. Early detection of this pathology is essential for a good prognosis; otherwise, the disease can lead to the development of fibrous tissue, calcifications and tie downs , making impossible the animal releasing into the wild, the primary goal of rehabilitation of wildlife.
Case 2: Lammergeier (Gypaetus barbatus)
On 13 April 2012, an adult female was admitted to the fauna recuperation centre of Vallcalent, Lleida (Spain). The admission examination revealed proper body condition, good hydration and alert mental status. Nevertheless, the animal put little weight on the left limb and leaning instead on the dorsum of the foot. Treatment consisted of antibiotics, anti-inflammatory and physiotherapy on the left leg. The three most distal phalanges of left finger III were amputated 5 days after admission because their condition worsened despite treatment. The animal remained in the rehabilitation centre with a good prognosis, the amputated finger correctly healed and the left leg appeared strong and toned, and the animal was using it normally.
For the histology analysis (Cases 2, 3), tissue samples were fixed in formalin, lightly decalcified with nitrogen oxide when necessary, trimmed and paraffin-embedded. Tissues were then sectioned at a thickness of 4 μm and stained with hematoxylin-eosin (H-E) for diagnosis purposes. After the amputation, the histopathological examination of left finger III revealed broad segments of full thickness dermal coagulative necrosis (Figure 2(4-6)). Keratinocytes had bright eosinophilic homogeneous cytoplasm, fused cell borders and streaming basophilic nuclei. Broad linear multifocal segments of deep dermis, panniculus and tendon sheaths had brightly eosinophilic, glassy and fragmented collagen fibres, separated by clear space, mucinous material and scattered clusters of heterophils, lymphocytes, plasma cells and macrophages. Some arterioles had mural aggregates of similar inflammatory cells and glassy walls. These findings of severe subacute cutaneous avascular necrosis and heterophilic dermatitis were consistent with a third-degree burn. The segmental pattern of the full thickness coagulative necrosis and the lack of external lesions until sometime after the animal was admitted into rehabilitation were suggestive of an electrical rather than heat burn . Electric current flows through areas of least resistance; hence, tissue is differentially affected [21, 22].
Even before amputation, the thermography detected the abnormal thermal pattern and lack of thermal symmetry in left fingers III and IV, as well as the worse prognosis for finger III, thus supporting the idea that this non-invasive technique can gain useful clinical insights into affected animals.
Case 3: Osprey (Pandion haliaetus)
On 16 March 2012, a juvenile male was admitted to GREFA. The clinical examination revealed an open, comminuted fracture of the left radius and ulna. Treatment included antibiotics, anti-inflammatories and propentofylline. On 24 March 2012, an intramedullary needle was surgically implanted into the left radius and ulna. After surgery, the animal’s condition continued to worsen; an oedema in the wing appeared and increased, and the animal could not stand up on its legs because of the neurovascular necrosis, which would have required amputation of the limb. Since amputation of the leg is incompatible with the hunting and feeding habits of this species, it was euthanised for humanitarian reasons on 28 March 2012 based on previous studies recommendations [23–25]. Then, necropsy was performed according to the protocol of Schmidt and Reavill [26, 27].
Necropsy revealed thinness, an oedema in the left wing, haematoma in ribs and keel, atrophied pectoral muscles, numerous clots distributed by all the vessels in lung and air sacs veins with yellowish deposits, thickened pericardium, myocardial greenish stain in the left ventricle, whitish injury in the apex, and liver yellowish and congestive encephalon. Cold oedemas mainly in the distal wing and coagulation in internal organs have been previously described as common necropsy findings in electrocuted birds . The wing fracture and haematomas in pectoral muscles and keel were probably secondary to the fall after colliding with power lines.
The most remarkable histopathological findings were in the liver. The hepatic parenchyma had broad multifocal fragmented areas of coagulative necrosis surrounded by abundant immature granulation tissue and numerous foamy macrophages and giant multinucleated cells. The viable hepatic parenchyma adjacent to the granulation tissue was disorganized and contained numerous packed small groups of oval cells arranged in tubules (regeneration) and separated by moderate amounts of mature fibrous tissue, haemosiderin and bile-laden macrophages, heterophils and lymphocytes. The hepatic coagulative necrosis was suggestive of blunt trauma, often associated with electrocution [28–30]. Tissue is damaged due to the direct effects of the electrical current as it dissipates through organs [29, 30]. Moreover, as this injured animal was located close to power lines, electrocution was highly suggestive.
The anamnesis, the veterinary examinations, and the histopathological analyses and necropsies, when performed, confirmed electrocution of these three animals. Thermographically, temperature reduction caused by electrocution was detected since the animals’ admission, and prior to macroscopic lesions appearing. Additionally, the comparison made of the thermographs before and after physiotherapy revealed that thermography may help predict tissue damage prognosis and guide rehabilitation efforts.
Thermographically, injured tissues were cooler than the corresponding healthy ones and the thermal pattern was altered, which showed that thermal symmetry and thermal distribution are more important parameters than absolute temperature. Mabuchi et al.  drew the same conclusion when assessing the application of thermography for clinical diagnoses in humans. However, as reference absolute values are currently lacking, each clinical case should be studied carefully by performing thermography before and during rehabilitation in order to follow the thermal evolution. Although thermography is the most rapid and non-invasive tool for electrocution diagnosis, histopathology should be used to confirm the suspicious cases.
Thermography is a rapid, stress-free and objective tool that allows: the early detection of asymmetry temperature distribution and lesions consistent with electrocution without necropsy or amputation; the determination of areas damaged by the entry and exit of an electric current; the assessment of the animal’s clinical status; the diagnosis of pathologies associated with electrocution, such as fractures and patagium retraction; and the evaluation of how lesions evolve with different treatments; all of which can contribute to more effective treatment.
The authors acknowledge the staff and volunteers of the GREFA and Vallcalent rehabilitation centres for their assistance and devotion. We acknowledge the Kirma physiotherapy centre for their collaboration in the treatment of the Lammergeier. Mar Melero is the recipient of a PhD student grant from the Complutense University of Madrid.
- Molina-López RA, Casal J, Darwich L: Causes of morbidity in wild raptor populations admitted at a wildlife rehabilitation centre in Spain from 1995–2007: a long term retrospective study. PLoS One. 2011, 6 (9): e24603-10.1371/journal.pone.0024603.PubMedPubMed CentralView ArticleGoogle Scholar
- Burfield IJ: The conservation status and trends of raptors and owls in Europe. Ambio. 2008, 37 (6): 401-407. 10.1579/0044-7447(2008)37[401:TCSATO]2.0.CO;2.PubMedView ArticleGoogle Scholar
- González LM, Margalida A, Mañosa S, Sánchez R, Oria J, Molina JI, Caldera J, Aranda A, Prada L: Causes and spatio-temporal variations of non-natural mortality in the vulnerable Spanish imperial eagle Aquila adalberti during a recovery period. Oryx. 2007, 41 (4): 495-502.View ArticleGoogle Scholar
- Guil F, Fernández-Olalla M, Moreno-Opo R, Mosqueda I, Gómez ME, Aranda A, Arredondo A, Guzmán J, Oria J, González LM, Margalida A: Minimising mortality in endangered raptors due to power lines: the importance of spatial aggregation to optimize the application of mitigation measures. PLoS One. 2011, 6 (11): e28212-10.1371/journal.pone.0028212.PubMedPubMed CentralView ArticleGoogle Scholar
- Bevanger K: Biological and conservation aspects of bird mortality caused by electricity power lines: a review. Biol Conserv. 1998, 86 (1): 67-76. 10.1016/S0006-3207(97)00176-6.View ArticleGoogle Scholar
- Janss GFE, Ferrer M: Avian electrocution on power poles: European experiences. In Birds and power lines: collision, electrocution and breeding. Edited by: Ferrer M, Janss GFE. Madrid: Quercus; 1999:145-164.Google Scholar
- Lehman RN, Kennedy PL, Savidge JA: The state of the art in raptor electrocution research: a global review. Biol Conserv. 2007, 136 (2): 159-174. 10.1016/j.biocon.2006.09.015.View ArticleGoogle Scholar
- Routh A, Sanderson S: Waterfowl. In In Handbook of Avian Medicine. 2nd edition. Edited by Tully TN, Dorrestein GM, Jones AK. Oxford: Butterworth-Heinemann; 2009:234––265.Google Scholar
- Üzün I, Akyildiz E, İnanici MA: Histopathological differentiation of skin lesions caused by electrocution, flame burns and abrasion. Forensic Sci Int. 2008, 178 (2–3): 157-161.PubMedView ArticleGoogle Scholar
- Mc Cafferty DJ: The value of infrared thermography for research on mammals: previous applications and future directions. Mamm Rev. 2007, 37 (3): 177-255. 10.1111/j.1365-2907.2007.00107.x.View ArticleGoogle Scholar
- Mabuchi K, Chinzei T, Fujimasa I, Haeno S, Motomura K, Abe Y, Yonezawa T: Evaluating asymmetrical thermal distributions through image processing. IEEE Eng Med Biol Maq. 1998, 17 (4): 47-55. 10.1109/MEMB.1998.687963.View ArticleGoogle Scholar
- Renkielska A, Nowakowski A, Kaczmarek M, Dobke MK, Grudzinski J, Karmolinski A, Stojek W: Static thermography revisited – an adjunct method for determining the depth of the burn injury. Burns. 2005, 31 (6): 768-775. 10.1016/j.burns.2005.04.006.PubMedView ArticleGoogle Scholar
- Tepper M, Neeman R, Milstein Y, David MB, Gannot I: Thermal imaging method for estimating oxygen saturation. J Biomed Opt. 2009, 14 (5): 054048-10.1117/1.3251036.PubMedView ArticleGoogle Scholar
- Dirección General para la Biodiversidad. Ministerio de Medio Ambiente: Catálogo Nacional de Especies Amenazadas. 2011, Madrid: Listado de taxones por categorías de amenazas. R.D. 439/1990, R.D. 139/2011Google Scholar
- IUCN. 2012, http://www.iucnredlist.org, . IUCN Red List of Threatened Species.Google Scholar
- Morgan PB, Soh MP, Efron N: Potential applications of ocular thermography. Optom Vis Sci. 1993, 70 (7): 568-576. 10.1097/00006324-199307000-00008.PubMedView ArticleGoogle Scholar
- Colegrave N, Engel J, Plowman AB: Randomisation tests. In Zoo research guidelines: Statistics for typical zoo datasets. Edited by: Plowman AB. 2006, London: BIAZA, 7-16.Google Scholar
- Orosz SE, Ensley PK, Haynes CJ: Avian surgical anatomy: thoracic and pelvic limbs. Philadelphia: W.B. Saunders:1992.Google Scholar
- Jato S, Otero I, González F, López I, Mendoza JL: Patagium rehabilitation treatment in wild birds following long-term wing immobilization. Wildl Rehabilitation Bull. 2011, 29 (2): 33-41.Google Scholar
- Gross TL, Ihrke PJ, Walder EJ, Affolter VK: Necrotizing diseases of the epidermis. In Skin Diseases of the Dog and Cat, Clinical and Histopathologic Diagnosis. 2nd edition. Ames: Blackwell Science; 2005:94–97.View ArticleGoogle Scholar
- Thomsen HK, Danielsen L, Nielsen O, Aalund O, Nielsen KG, Karlsmark T, Genefke IK: Early epidermal changes in heat- and electrically injured pig skin. I. A light microscopic study. Forensic Sci Int. 1981, 17 (2): 133-143. 10.1016/0379-0738(81)90005-0.PubMedView ArticleGoogle Scholar
- Danielsen L, Gniadecka M, Thomsen HK, Pedersen F, Strange S, Nielsen KG, Petersen HD: Skin changes following defibrillation. The effect of high voltage direct current. Forensic Sci Int. 2003, 134 (2–3): 134-141.PubMedView ArticleGoogle Scholar
- Myers DA: Common procedures and concerns with wildlife. Vet Clin North Am Exot Anim Pract. 2006, 9 (2): 437-460. 10.1016/j.cvex.2006.03.005.PubMedView ArticleGoogle Scholar
- Baer CK: Guidelines for euthanasia of nondomestic animals. Lawrence: American Association of Zoo Veterinarians (AAZV) 2006.Google Scholar
- American Veterinary Medical Association: AVMA Guidelines on Euthanasia. Formerly report of the AVMA panel on euthanasia. Schaumburg: AVMA: 2007.Google Scholar
- Schmidt RE, Reavill DR: A practitioner’s guide to avian necropsy. Necropsy CD, Zoological Education Network, ISBN: 2003,0-9706395-0-3Google Scholar
- Rae MA: Practical avian necropsy. Semin Avian Exot Pet Med. 2003, 12 (2): 62-70. 10.1053/S1055-937X(03)80003-8.View ArticleGoogle Scholar
- Koumbourlis AC: Electrical injuries. Crit Care Med. 2002, 30 (11 Suppl): 424-430.View ArticleGoogle Scholar
- Ritenour AE, Morton MJ, McManus JG, Barillo DJ, Cancio LC: Lightning injury: a review. Burns. 2008, 34 (5): 585-594. 10.1016/j.burns.2007.11.006.PubMedView ArticleGoogle Scholar
- Fish RM, Geddes LA: Conduction of electrical current to and through the human body: a review. Eplasty. 2009, 9: e44.PubMedPubMed CentralGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.