Pseudomyotonia in Romagnola cattle caused by novel ATP2A1mutations
- Leonardo Murgiano†1,
- Roberta Sacchetto†2,
- Stefania Testoni3,
- Tiziano Dorotea2,
- Francesco Mascarello2,
- Rocco Liguori4,
- Arcangelo Gentile5 and
- Cord Drögemüller1Email author
© Murgiano et al.; licensee BioMed Central Ltd. 2012
Received: 14 June 2012
Accepted: 25 September 2012
Published: 9 October 2012
Bovine congenital pseudomyotonia (PMT) is an impairment of muscle relaxation induced by exercise preventing animals from performing rapid movements. Forms of recessively inherited PMT have been described in different cattle breeds caused by two independent mutations in ATP2A1 encoding a skeletal-muscle Ca2+-ATPase (SERCA1). We observed symptoms of congenital PMT in four related Romagnola beef cattle from Italy and evaluated SERCA1 activity and scanned ATP2A1 for possible causative mutations.
We obtained four PMT affected Romagnola cattle and noted striking clinical similarities to the previously described PMT cases in other cattle breeds. The affected animals had a reduced SERCA1 activity in the sarcoplasmic reticulum. A single affected animal was homozygous for a novel complex variant in ATP2A1 exon 8 (c.[632 G>T; 857 G>T]). Three out of four cases were compound heterozygous for the newly identified exon 8 variant and the exon 6 variant c.491 G>A(p. Arg146Gly), which has previously been shown to cause PMT in Chianina cattle. Pedigree analysis showed that the exon 8 double mutation event dates back to at least 1978. Both nucleotide substitutions are predicted to alter the SERCA1 amino acid sequence (p.[(Gly211Val; Gly284Val)]), affect highly conserved residues, in particular the actuator domain of SERCA1.
Clinical, biochemical and DNA analyses confirmed the initial hypothesis. We provide functional and genetic evidence that one novel and one previously described ATP2A1 mutation lead to a reduced SERCA1 activity in skeletal muscles and pseudomyotonia in affected Romagnola cattle. Selection against these mutations can now be used to eliminate the mutant alleles from the Romagnola breed.
Bovine congenital pseudomyotonia (PMT) is an impairment of muscle relaxation induced by exercise that prevents animals from performing rapid movements. So far it has been described in Italian Chianina beef cattle , in Belgian Blue cattle (named muscular dystonia type II)  and, as a single case, in a Dutch improved Red and White cross-breed calf . The observed muscular stiffness in PMT is due to a delayed relaxation of muscles of the fast twitch (type II) fibers. The biochemical mechanism underlying this dysfunction is a prolonged elevation in cytoplasmic free Ca2+ concentration, resulting from a deficiency of the SERCA1 protein. SERCA1 is a skeletal muscle Ca2+-ATPase or Ca2+ pump which is responsible for the Ca2+ re-uptake into the sarcoplasmic reticulum after muscle contraction . Bovine PMT disease strongly resembles the inherited Brody myopathy in humans , a condition of exercise-induced impairment of skeletal muscle relaxation, stiffness and cramps, caused by mutations of ATP2A1 coding for SERCA1 .
In Chianina cattle, we have described PMT as autosomal recessive inherited disorder caused by a missense mutation (c.491 G>A) in exon 6 of ATP2A1. This mutation leads to a p. Arg164His substitution in a highly conserved region of the SERCA1 protein reducing its activity [4, 7]. In muscular dystonia type II affected Belgian Blue cattle, Charlier et al.  reported an ATP2A1 missense mutation (c.1675C>T) leading to an amino acid substitution (p. Arg559Cys) in a highly conserved nucleotide binding domain. The same mutation was also reported to cause PMT in a single case of crossbred Dutch Improved Red cattle .
We observed four cases of PMT in Romagnola cattle, a white-coated Italian beef breed. The purpose of this study was to characterize the phenotype in comparison to the known PMT forms of other cattle breeds. Since we observed similar clinical signs, histological and biochemical features as in the formerly described Chianina cattle with PMT, we proceeded with a mutation analysis of ATP2A1 and detected mutations, which most likely cause the disease in Romagnola cattle.
Results and discussion
All four affected animals (two males, two females) showed congenital exercise induced muscle contraction that prevented them from performing muscular activities of greater intensity than a simple walk at a slow pace (see Additional file 1). When startled, or forced to move faster, the muscles ‘froze up’ temporarily, inducing rigidity and uncoordinated gait without signs of pain. If these stimulations were prolonged, the muscular stiffness became so pronounced that the animals fell to the ground. After a few seconds, the muscles relaxed and the animals regained their ability to get up and move. Altogether the clinical picture strongly resembled the signs of PMT in Chianina cattle . At rest electromyography (EMG) investigations of the triceps surae and gluteobiceps muscles and paraspinalis muscles of the thoracic region showed no spontaneous activity. Apart from the muscular symptoms the affected animals didn’t show any other clinical signs: They were bright, alert and in good body condition. Two of the animals (case 1 and 4) were 2 months old at the moment of the examination; the other two were 3 months old.
Sarcoplasmic reticulum microsomal fractions probed with anti-SERCA1 monoclonal antibodies showed that the amount of SERCA1 protein was decreased in the pathological samples with respect to control samples (Figure 2B). Although the SERCA1 staining was variable in PMT affected muscles, the reduction in SERCA1 protein density consistently correlated with the decrease in Ca2+-ATPase activity. Case 3 that showed the faintest SERCA1 band exhibited the most severe pathology, as demonstrated by active muscle damage and regeneration (Figure 1). Sarcoplasmic reticulum fractions from pathological and control samples were also probed with specific antibodies against protein markers of junctional (calcium release channel/ryanodine receptor 1) and non-junctional (sarcalumenin and its splice variant 53 kDa glycoprotein) sarcoplasmic reticulum membranes. As in PMT affected Chianina cattle , sarcoplasmic reticulum fractions from PMT affected Romagnola cattle did not differ in content of either junctional or non-junctional membrane markers, indicating that only SERCA1 is selectively affected.
ATP2A1 mutation analysis
ATP2A1 genotypes of four pseudomyotonia affected Romagnola cattle and their direct relatives
exon 6 (c.491 G>A)
exon 8 (c.632 G>T)
exon 8 (c.857 G>T)
Dam of case 1
Sire of case1
Dam of case 2
Sire of case 2 and 3
Dam of case 3
Dam of case 4
Sire of case 4
Compound heterozygous mutations causing recessively inherited genetic diseases are well known in human genetics but not described commonly in purebred domestic animals. As recently shown for PMT in Chianina cattle, the spreading of a single founder mutation after intense use of closely related artificial insemination carrier sires typically explains outbreaks of genetic disease in cattle populations . The origin of the mutation in the ancestors of both breeds seems not probable to the authors, so we assume that the most likely scenario for the presence of the exon 6 mutation in Romagnola cattle is an accidental introgression of a Chianina PMT carrier animal in the ancestry of Neff (Figure 3).
Possible impact of the SERCA1 mutations
We considered structure and function of SERCA1 to explain possible effects of the mutations causing PMT in Romagnola cattle. The SERCA1 protein consists of three cytosolic domains (see Figure 5) involved in the transmission of the major conformational changes . The intracellular calcium pump is located in the sarcoplasmic reticulum and transfers Ca2+ from the cytosol of the cell to the lumen of the sarcoplasmic reticulum at the expense of ATP hydrolysis during muscle relaxation. The mechanism of the transport has been explained in term of a cycle characterized by two conformational states: E1 (Ca2+-bound form, high calcium affinity, protein works as a kinase phosphorylating Asp351) and E2 (Calcium released, Ca2+ affinity is low, protein works as a phosphatase) . Dramatic changes in orientation of the domains in the transition from E1 to E2 were described [11, 13]. In detail, the actuator domain is described as subjected to several rotations and to interact with phosphorylation and nucleotide binding domain . Furthermore, there have to be movements of the helices in the transmembrane region to generate pathways for the entry or the release of the Ca2+ ions .
In PMT affected Romagnola cattle the side chain hydrogen atoms of glycines 211 and 286 are substituted by the much larger aliphatic hydrophobic side chain of valine, which tends to be buried in the center of protein, away from the aqueous environment. In both cases, the substitutions could have a role in disfavoring the proper folding of the protein. Recently, the crystal structure of the bovine SERCA1 has been reported . The overall molecular model is very similar to that of the rabbit enzyme . The PMT causing mutations in SERCA1 described here occur in the actuator domain of SERCA1 and in the lumenal loop where the similarity between the bovine and rabbit crystal structure is very high.
The putative consequences of the mutations were also evaluated with the help of several software programs. In particular, they predicted the mutation as highly damaging, and causing loss of a turn and the extension of a beta-sheet as a consequence of the p. Gly211Val mutation, and possible loss of stability near a transmembrane domain. This could be probably detrimental to the protein functioning considering the great interaction of the actuator domain with other parts of the protein and the numerous movements the domain is subjected to (see Additional file 2). The significantly reduced SERCA1 activity implicates a partial loss of function of these mutations. However, the PMT affected Romagnola animals showed no clinically obvious different disease signs compared to affected Chianina cattle. The impact of the mutations reported in this paper are not directly comparable with the p. Arg559Cys reported in Belgian Blue  since it consists in the substitution of a residue interacting directly with a nucleotide. Additionally, it is difficult to compare the effect of the mutations in cattle to the ones found in Brody’s disease, since the reported human causative mutations [17, 18] affect other parts of the SERCA1 protein and are neither observed in non-human species .
In pseudomyotonia affected animals the Ca2+-ATPase activity of sarcoplasmic reticulum membranes (SERCA1) was markedly decreased compared to control animals. We were able to successfully detect one known and one novel complex ATP2A1 variant associated with the cases of pseudomyotonia. Some cases showed compound heterozygous genotypes. This study is, to the author’s knowledge, the first time that compound heterozygosity for different mutations in a single gene has been reported to cause genetic disorder in livestock. Furthermore, the knowledge of the new amino acid substitutions and their effect on the phenotype provides further insight into the function of the skeletal muscle SERCA1 calcium pump. Finally, selection against these mutations can now be used to eliminate the mutant alleles from the Romagnola breed.
All animal work has been conducted according to the national and international guidelines for animal welfare. The cattle owner agreed that the samples can be used for our study. The data were obtained during diagnostic procedures that would have been carried out anyway. This is a very special situation in veterinary medicine. As the data are from client-owned cattle that underwent veterinary exams, there was no “animal experiment” according to the legal definitions in Italy.
Blood and tissue samples were taken from the affected animals and from a total of seven parents (see Table 1 and Figure 3). Genomic DNA was isolated using the DNeasy Blood & Tissue Kit (Qiagen) according to the manufacturer’s protocol. In addition, archived DNA samples of 205 Romagnola bulls and 100 animals from 20 different cattle breeds (Angus (n = 5), Aubrac (n = 1), Belgian blue (n = 3), Blonde d’Aquitaine (n = 2), Brown Swiss (n = 10), Charolais (n = 5), Chianina (n = 10), Eringer (n = 5), Galloway (n = 2), Hereford (n = 3), Scotish Highland (n = 4), Holstein (n = 10), Jersey (n = 3), Limousin (n = 10), Montbéliarde (n = 4),, Piedmontese (n = 2), Pinzgauer (n = 5), Salers (n = 1), Simmentaler (n = 10), Tyrolean Grey (n = 5)) were used for genotyping the ATP2A1 exon 8 variants.
We obtained muscle biopsies of the semimembranosus muscle from case 2 and case 3 (Figure 3). Muscle samples were frozen in cold isopentane and sections (10 μm) were cut in a cryostat. Serial cryostat sections were stained by: Hematoxylin and Eosin (H&E), Gomori’s trichrome, periodic acid-Schiff (PAS), succinic dehydrogenase (SDH), cytochrome oxidase (COX), or were immunostained by incubating with polyclonal antibodies (dilution 1:200) to neonatal myosin heavy chain (MHC) isoform , followed by incubation with secondary antibody conjugated with peroxidase (Dako, Milano, Italy). Reaction was visualized with the Envision method (Dako). Images were acquired with a bright field microscope (Olympus Vanox AH-3, Japan), equipped with video camera and analyzed with image analysis software (Olympus DP-software-70).
A crude microsomal fraction enriched in content of sarcoplasmic reticulum membranes was isolated by differential centrifugation from muscle biopsies from semimembranosus muscle of three PMT affected Romagnola animals. Protein concentration was determined by the method of Lowry et al. . The Ca2+-ATPase activity of sarcoplasmic reticulum microsomal fraction, has been measured at optimum pCa (pCa5), in the presence of Ca2+-ionophore A23187. Equal quantities (3 μg/lane) of crude sarcoplasmic reticulum microsomal fractions were separated by 5-10% SDS-PAGE and immunoblotting was performed as described before .
The pedigrees of all animals included in the study were evaluated on the basis of the database of the Italian beef cattle breeding organization .
Sequencing of all 22 coding exons including flanking intronic sequences of ATP2A1 was performed for all four cases as described before . The PMT causing mutations in all the relatives and controls were genotyped by direct re-sequencing of two PCR products containing bovine ATP2A1 exon 6 and exon 8, respectively.
Protein sequence analysis
Multiple sequence alignment was performed with ClustalW . Impact of the mutations on the protein stability and structure was predicted with the following software tools: PolyPhen2 ; NetTurnP ; NetSurfP ; the Chou&Fasman secondary structure prediction server CFSSP ; PoPMuSiC ; Phyre2 . For transmembrane domain prediction we used THMM  and THMpred .
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