Figure 1

Histopathological examination and immunohistochemistry of muscle biopsies from PMT affected Romagnola cattle. Transversal sections from case 3 muscle biopsies were stained with H&E. A pale enlarged fiber (A) and pale fibers invaded by mononuclear cells (B) are indicated by arrows. Serial transversal sections from case 3 muscle biopsies were stained with H&E (C) or immunostained with anti-neonatal MHC isoform antibodies. Regenerating fibers at different stages of development (arrows) and small sized fibers (arrowheads) positive to anti neonatal MHC isoform antibodies, are indicated (D). Transversal sections of muscle biopsies of case 2 were stained with COX-SDH (E) and PAS (F). The polygonal honeycomb-like staining pattern is shown in more detail in the inset of panel F. (Scale bars: 50 μm. Scale bar inset: 5 μm).