The wild boar (Sus scrofa) Lymphocyte function-associated antigen-1 (CD11a/CD18) receptor: cDNA sequencing, structure analysis and comparison with homologues
BMC Veterinary Research volume 3, Article number: 27 (2007)
The most predominant beta2-integrin lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18, alphaLbeta2), expressed on all leukocytes, is essential for many adhesive functions of the immune system. Interestingly, RTX toxin-producing bacteria specifically target this leukocyte beta2-integrin which exacerbates lesions and disease development.
This study reports the sequencing of the wild boar beta2-integrin CD11a and CD18 cDNAs. Predicted CD11a and CD18 subunits share all the main structural characteristics of their mammalian homologues, with a larger interspecies conservation for the CD18 than the CD11a. Besides these strong overall similarities, wild boar and domestic pig LFA-1 differ by 2 (CD18) and 1 or 3 (CD11a) substitutions, of which one is located in the crucial I-domain (CD11a, E168D).
As most wild boars are seropositive to the RTX toxin-producing bacterium Actinobacillus pleuropneumoniae and because they have sustained continuous natural selection, future studies addressing the functional impact of these polymorphisms could bring interesting new information on the physiopathology of Actinobacillus pleuropneumoniae-associated pneumonia in domestic pigs.
Cell adhesion receptors play crucial roles in multicellular organisms by mediating the direct cell/cell or cells/extracellular matrix proteins interactions. These molecular interactions condition the structural integrity of cells and tissues and contribute to the signalling transduction intervening in the cellular dynamic . Cell adhesion receptors are subdivided in several membrane-associated protein families, including integrins, cadherins, immunoglobulin superfamily cell adhesion molecules, selectins, and syndecans. Integrins are a family of cell surface adhesion and signalling glycoproteins made up of non-covalently associated 120–180 kDa α and 90-110kDa β subunits . There are 19 distinct α subunits and 8 β subunits that are combined to form 25 different heterodimeric receptors . Each subunit possesses (i) a large extracellular N-terminal domain associating with that of the companion subunit to form the integrin headpiece which contains the ligand binding site, (ii) a single transmembrane stretch, and (iii) a short cytoplasmic C-terminal tail which mediates interactions with cytoskeleton and signalling proteins [1, 3, 4].
Within the integrin family, the leukocyte-specific β2-integrins (CD11/CD18) include four members sharing in common the β2 subunit (CD18) : (i) the most predominant is the lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18, αLβ2) on all leukocytes ; (ii) the CD11b/CD18 (Mac-1, CR3, αMβ2) mainly on activated granulocytes and tissues macrophages ; (iii) the CD11c/CD18 (gp150/95, CR4, αXβ2, Leu-M5) also on activated granulocytes and tissues macrophages , and (iv) the CD11d/CD18 (αDβ2) abundant on the CD8+ lymphocyte and on macrophages [9–11]. These receptors are essential for an effective immune system as observed in repeated infections associated with the lymphocyte adhesion deficiency (LAD) type I syndrome, a disease due to mutations in the β2 (CD18) subunit gene leading to the lack of functional β2 integrins on the membrane surface of leukocytes [12–14].
The β2-integrin LFA-1 is essential for the following functions of the immune system [15–20] : (i) interaction between lymphocytes, (ii) interaction between T-cells and antigen presenting cells, (iii) adhesion of naïve lymphocytes to post-capillary high endothelial venules of secondary lymphoid tissues, (iv) adhesion of leukocytes to activated endothelium at sites of inflammation for extravasation, (v) control of cell differentiation and proliferation, and (vi) antibody-dependent killing by natural killer cells and granulocytes. Leukocyte LFA-1-mediated adhesion is engaged via the binding of the LFA-1 in an activated conformational state to membrane proteins, the so-called intercellular adhesion molecules (ICAM)-1 to -5 and the junctional adhesion molecule (JAM)-A .
Interestingly, several pathogens target the leukocytes β2-integrins which leads to lesions and disease development . Several studies have highlighted the central role of LFA-1 in the pathogenesis of diseases caused by repeats-in-toxin (RTX)-producing bacteria. The virulence of Aggregatibacter (Actinobacillus) actinomycetemcomitans (localised aggressive periodontitis in humans), Mannheimia haemolytica (pneumonia in cattle), and pathogenic strains of Escherichia coli (extraintestinal infections) has been associated with a ligand/receptor interaction between their respective RTX toxin (LtxA, LktA, and HlyA) and the CD11a/CD18 receptor resulting in leukocytes alterations [23–26]. This interaction triggers synthesis and release of a wide array of cytokines and chemoattractants by the leukocytes that exacerbate inflammation and ultimately results in a much greater leukolysis worsening the lesions [25, 27]. Actinobacillus pleuropneumoniae, a causative agent of pleuropneumonia in domestic pigs (Sus scrofa domestica), responsible for economic losses and antibiotic usage in the pork industry, also produces RTX toxins (ApxIA, -IIA, -IIIA, and -IVA) [28, 29]. We therefore hypothesize that the pathogenesis of this disease similarly relies on an interaction with the Sus scrofa domestica LFA-1, whose CD11a (αL) and CD18 (β2) subunits have been well characterised [30, 31]. On the basis of the report that approximately 50% of wild boars in their natural environment are serologically positive for Actinobacillus pleuropneumoniae  and because these wild pigs sustain losses due to natural selection pressure, we hypothesize that some LFA-1 molecular peculiarities conferring resistance to wild boars could have been selected. In this context, the purpose of this study was to report the sequence and analysis of the cDNAs encoding wild boar LFA-1 (WbCD11a/WbCD18) and to point out the wild boar LFA-1 specificities that might confer resistance to Actinobacillus pleuropneumoniae-associated pneumonia.
Results and discussion
Characterization of WbCD11a-encoding cDNA and deduced amino-acid sequence
The WbCD11a cDNA sequence contains an ORF of 3519 bp [GenBank:EF585976] that codes for 1172 aa (Fig. 1). Starting from the N-terminal end, the 1172 aa mature WbCD11a contains a 23-residue putative leader peptide (M1-S23), an extracellular domain of 1064 residues (Y24-D1086), a single hydrophobic transmembrane region of 24 residues (M1087-Y1110) and a short cytoplasmic tail of 62 residues (K1111-A1172) (Fig. 1). Six N-linked putative glycosylation sites (Asn-Xaa-Ser/Thr) are found in the extracellular domain (Fig. 1). The WbCD11a possesses 22 cysteine residues, among which one is located in the cytoplasmic tail (Fig. 1). A subset of integrin α chains (α1, α2, α10, α11, αD, αE, αL, αM and αX), including CD11a, contains a I-domain (for Inserted domain, also called αLI-domain or αLA-domain) that is homologous to the family of von Willebrand Factor (vWF) A-type domains and to cartilage matrix protein [33, 34]. The I-domain has been associated with ligand binding. Its three-dimensional structure consists of a five-stranded parallel β-sheet core surrounded on both faces by seven α-helices (Fig. 1). A short antiparallel strand occurs on one edge of this sheet . The I-domain (I149-D331) contains a metal ion-dependent adhesion site (MIDAS) (residues D160-S164, T229, D262) [35, 36] (Fig. 1). The I-domain crystallisation has demonstrated that a "closed" (low affinity) and an "open" (high affinity) forms exist and that the major conformational changes during transition from the closed to open states include a rearrangement of the cation-coordinating residues in the MIDAS site, accompanied by a small inward movement of the α1 helix and a large downward shift of the mobile C-terminal α7 helix . The extracellular domain of WbCD11a contains seven internal repeats (FG-GAP) (G40-T89, S90-E147, S348-R398, A399-Q453, G455-D511, G513-F573, I576-P628) that surround the I-domain (Fig. 1) [38, 39]. The degree of identity is highest among the three COOH-terminal repeats (18–31%) and their central region (D466-E474, D528-D536 and D588-D596) is similar to the EF hand divalent cation-binding motifs (DCBM) of troponin C, parvalbumin and galactose binding protein  (Fig. 1). All the cysteine residues and all but one N-glycosylation sites are found outside the I-region and divalent cation binding motifs (Fig. 1), consistent with the hypothesis that these regions may undergo conformational changes important in ligand binding [38, 40]. Between the FG-GAP 7 and the transmembrane domain stands the thigh domain (M629-K766), the genu (N767-C774), and the CALF domains (E775-L920, N921-D1086) . The cytoplasmic portion of WbCD11a contains four potential phosphorylation sites and also a conserved "G1113FFKR" basic sequence near the transmembrane region (Fig. 1). The integrins become constitutively active when this sequence is deleted. The "G1113FFKR" motif thus normally fixes the integrins in an inactive state [11, 42].
Beside the complex mechanisms of affinity/avidity regulation of the integrins, the existence of several isoforms issued from alternative splicing complicates the biological understanding of these glycoproteins . Previously, we have characterised two different forms of PoCD11a due to the presence of a supplementary "cag" codon that codes for a glutamine (Q) in position 744 [GenBank:DQ013284, GenBank:DQ013285] . The addition of a Gln at the same position was also observed in the human (Q746) [GenBank:NM_002209, GenBank:AY892236], the simian (Q746) , ovine (Q743)  and caprine (Q743) [GenBank:AY773018, GenBank:AY773019] CD11a cDNAs (Fig. 2). This addition located in the thigh domain of the extracellular part of CD11a, just above the genu, increases the length of an α-helix in the PoCD11a according to the GORIV bioinformatic program. Until now, it was not clear whether this addition represented two alleles or was generated by an alternative splicing. We have recently cloned and sequenced a third PoCD11a form characterised by an insertion of 27 amino acids at the same position (P744EPLQLSSTSSAASATLSRLPLLCAQQ770) [GenBank:DQ474234] which is predicted to lengthen the α-helix further. The nucleotidic sequence of this insertion corresponds to that of the 3'end of the adjacent bovine and human intron 18 (79% and 70% of identity respectively), suggesting that the insertion of the glutamine or of the 27 amino acids-long stretch in position 744 of the thigh domain comes from an alternative splicing rather than from different alleles. Although these two insertions were not observed in the WbCD11a yet and because of the between-species conservation of this potential alternative splicing site, we hypothesize that it can have a biological importance for the mature CD11a, for example, in regulating the ligand binding and signaling activity.
WbCD11a comparison among species
Overall, the general organization of wild boar (Sus scrofa), porcine (Sus scrofa domestica) , bovine (Bos taurus) , ovine (Ovis aries) , caprine (Capra hircus) , human , simian (Pan troglodytes) , canine (Canis familiaris) [GenBank:XM_547024], rat (Rattus norvegicus) [GenBank:NP_001029170], and murine (Mus musculus)  CD11a proteins is quite similar (Fig. 2). Comparison between mature WbCD11a sequence and its porcine, bovine, ovine, caprine, human, simian, canine, rat and murine counterparts shows respectively overall 99%, 77%, 77%, 77%, 76%, 76%, 76%, 70% and 69% identity, and 99%, 87%, 86%, 86%, 86%, 86%, 85%, 81%, and 80% similarity (BLOSUM62table) (Table 1). The highest identity is found for the "G1113FFKR" motif, the genu, the MIDAS motif and the transmembrane region and the lowest for the cytoplasmic tail and the putative signal peptide (Table 1). Although DCBM3 presents a weak identity, its similarity score is high. The "G1113FFKR" sequence is highly conserved which is consistent with the stabilizing role of this motif for the alpha/beta complex, possibly because of its direct involvement in heterodimer formation . The genu seems to play a key role in the activation of the LFA-1 through the deployment of the receptor  and its great conservation is therefore not surprising. The high conservation of the MIDAS and the putative cation binding motifs is consistent with an involvement of these regions in the functional activity of the LFA-1 α subunit, as suggested by the requirement of Mg2+ and Ca2+ for CD11a/CD18-dependent cellular interactions  or binding to purified ICAM-1 [49, 50]. The transmembrane region also shows a high degree of conservation, probably due to shared physicochemical and functional constraints. Indeed, residues lying in the membrane first have to possess a hydrophobic character to warrant liposolubility, which is confirmed by the presence of many leucine residues (Fig. 2). Secondly, bidirectional integrin signalling (inside-out and outside-in) is accomplished by transmission of information across the plasma membrane . By contrast, the low conservation of the COOH-terminal part of the cytoplasmic tail suggests that it is not required to guarantee adequate functioning of LFA-1. This is in agreement with the observation that truncation of the LFA-1 α subunit cytoplasmic domain has no effect on binding to ICAM-1, whereas binding is markedly diminished by β subunit cytoplasmic domain truncation . The "I149KGN" motif known to participate in the binding to ICAM-3  shows a high degree of conservation (Fig. 2). The amino acid P215, participating to the binding to ICAM-1  is highly conserved too (Fig. 2). Residue E333, located in the linker following the I domain which is critical for communication with the β2 I-like domain, rolling, integrin extension and activation by Mn2+  is logically strictly conserved too. The K1120 residue, critical for Rap1-dependent LFA-1 activation and affinity up-regulation  is also strongly conserved (Fig. 2). Every cysteine residue in the mature WbCD11a is present at the same location in bovine, ovine, human, simian, and murine CD11a, which is consistent with a role in maintaining the global structure of the protein. Finally, of six potential Asn-glycosylation sites in WbCD11a, the ones present at amino acids 186 and 724 are strictly conserved (Fig. 2). In addition, although WbCD11a sequences were obtained from only four wild boars, one of them was heterozygous. Both alleles differed from those found in pigs by a G736A substitution. One allele displayed 2 additional substitutions compared to pigs: E168D (in the I-domain) and D621E (in the FG-GAP7, Fig. 1). According to the BLOSUM 62 table, these substitutions are theoretically predicted to have a weak impact on the general structure of CD11a. However, these two wild boar-specific CD11a isoforms might display an altered/improved function compared to those described among domestic pigs .
Characterization of WbCD18-encoding cDNA and deduced amino-acid sequence
The cDNA sequence of WbCD18 contains an ORF of 2310 bp [GenBank:EF585977] that codes for 769 aa (Fig. 3). The mature WbCD18 contains a 22 aa putative leader peptide (M1-S22), an extracellular domain of 679 residues (Q23-N700), a single hydrophobic transmembrane region of 23 residues (I701-W723) and a short cytoplasmic tail of 46 residues (K724-R769) (Fig. 3). Starting from the N-terminal end, the extracellular region successively contains a cysteine-rich repeats-containing analogue of the so-called  plexin-semaphorin integrin domain (Q23-V74) , a spacer (A75-K123) [55, 56], an inserted I-like domain of 240 amino acids (G124-L363), a mid-region (S364-C445) that fold-up with the spacer to form the hybrid domain [55, 56], a cysteine-rich region containing four EGF-like regions (C449-E482, C483-E535, C536-Q574, and C575-T613) [39, 55, 57], and a C-terminal β-tail domain  (D614-N700) (Fig. 3). A putative metal ion-dependent adhesion site (MIDAS)-like DXSXS motif (D134-M139), an adjacent site to MIDAS (ADMIDAS) (D141, D142, E347), and a ligand-induced metal-binding site (LIMBS) (D173, N229, D231, P233, E234) motif are predicted within the I-like domain . The cytoplasmic tail contains the binding sites for cytohesin, Rack1 (K724VLT), and α-actinin (K736RFEKEKLKSQ) . Overall, the protein contains 58 cysteine residues and 5 N-linked putative glycosylation sites (Asn-X-Thr/Ser), all located within the extracellular region (Fig. 3).
WbCD18 comparison among species
Overall, the general organization of wild boar (Sus scrofa), porcine (Sus scrofa domestica) , bovine (Bos taurus) , water buffalo (Bubalus bubalis) (GenPept AAW29104), caprine (Capra hircus) , ovine (Ovis aries and Ovis canadensis) [62, 63], human , canine (Canis familiaris) , murine (Mus musculus) , rat (Rattus norvegicus) [GenBank:NM_001037780], chicken (Gallus gallus) , carp and channel catfish (Cyprinus carpio and Ictalurus punctatus) [GenBank:AB031070]  CD18 proteins is quite similar (Fig. 4). Sequence comparisons between WbCD18 and its porcine, bovine, water buffalo, caprine, ovine, human, canine, murine, rat, chicken, carp and channel catfish counterparts shows respectively, 99%, 88%, 88%, 88%, 88%, 87%, 83%, 81%, 81%, 80%, 62%, 49% and 48% identity, and 99%, 93%, 93%, 93%, 93%, 93%, 90%, 89%, 88%, 88%, 76%, 64% and 63% similarity (BLOSUM62 table) (Table 2). The MIDAS-like, ADMIDAS, LIMBS motifs, the I-like domain, the EGF-2 domain and the cytoplasmic tail have the highest identity while the putative peptide signal, the β-tail domain, and the EGF-1 show the lowest identity (Table 2). The very high interspecies conservation of the putative MIDAS-like, ADMIDAS, LIMBS, I-like domains and the cytoplasmic tail is consistent with an involvement of these regions in the functional activities of β2-integrins. Overall, the high evolutionary conservation of the I-like domain confirms its importance in β2-integrins functions, which is compatible with the observation that monoclonal antibodies binding epitopes mapped within this region inhibit binding of LFA-1 to ICAMs 1–3 . The maximum conservation being observed for the CD18 MIDAS-like motif, it is tempting to speculate that it plays a fundamental role in β2-integrin function. In this way, it was demonstrated that the C169PNKEKEC sequence conserved among mammalian species (Fig. 4) constitutively activates LFA-1 binding to ICAM-1 . LIMBS and ADMIDAS sites modulate binding of ligand to the MIDAS-like in the integrins that lack the αI domain [70–72] but the ADMIDAS seems also to regulate αL I domain affinity and to participate in the outside-in signalling . The high degree of conservation in the cytoplasmic tail, with many Ser, Thr, and Tyr residues, is compatible with the important role that phosphorylation of these residues plays in regulating adhesive activity  and with the observation that cytoplasmic domain truncation of CD18 markedly diminishes binding of LFA-1 to ICAM-1 . Importantly, it was shown that the phosphorylation of the highly conserved residues T758, T759 and T760 plays a crucial role in the activation of the receptor and the binding to ICAM-1 [59, 74]. In addition, the key residue F766 for binding to ICAM-1 is strictly conserved . Although EGF-1 possesses a weaker identity, its similarity is very high (Table 2). The weaker conservation of the β-tail domain could translate a less degree of importance of this domain for the CD18 function. Every cysteine residue in the wild boar extracellular portion of mature CD18 is present at the same location in CD18 from other species, which is consistent with a role in maintaining the global structure of the protein. Similarly, all five potential Asn-glycosylation sites observed in wild boar are present at the same location in other mammalian species. Wild boar-specific CD18 isoform is characterized by two amino acid substitutions compared to domestic pigs: G560S and A721V, which should not result in structural differences, according to BLOSUM 62 table, but might impact CD18 function.
This study reports the sequencing of the wild boar β2-integrin CD11a/CD18 subunits cDNAs. Predicted CD11a and CD18 subunits share all the main structural characteristics of their mammalian homologues, with a larger interspecies conservation for the CD18 than the CD11a. Besides these strong overall similarities, wild boar and domestic pig LFA-1 differ by 2 (CD18) and 1 or 3 (CD11a) substitutions, of which one is located in the crucial I-domain (CD11a, E168D). As most wild boars are seropositive to Actinobacillus pleuropneumoniae and because they have sustained continuous natural selection, future studies of the functional impact of these polymorphisms could bring interesting new information on the physiopathology of pneumonia in domestic pigs.
Total RNA from spleen of freshly slaughtered wild boars (Sus scrofa) was extracted with TRIzol (Invitrogen, USA) as described by the manufacturer.
Amplification and sequencing of the full length cDNA
Total RNA from spleen was reverse transcribed using Improm II (Promega). The full-length cDNA was then generated by long distance PCR using Taq and Pfu DNA polymerase from the High Fidelity PCR Enzyme Mix (Fermentas) with primers designed from the 5'- and 3'UTR of PoCD11a  and PoCD18 . The couple of primers used for amplification of WbCD11a and WbCD18 are the next: 5'GGTATGGTCCCTCCAGAAGC-3' (CD11a forward), 5'-GCAGGCTGAGTCCAGTCCTG-3' (CD11a reverse), 5'-GAGGTCTCCAGGACATCAAG-3' (CD18 forward) and 5'-TAGGGGTGCTTGGTGAAGAC-3' (CD18 reverse). The procedures recommended by the manufacturer were applied, with the following cycling parameters : 5 min at 94°C, then 35 cycles including (i) 30 s at 94°C, (ii) 45 s at 60°C and (iii) 3 min 45 s at 72°C, and a final extension at 72°C for 10 min. Resulting PCR products were purified using the NucleoSpin®ExtractII kit (Macherey-Nagel), and sequenced on a ABI-3100 Genetic Analyzer using the Big Dye terminator chemistry (Applied Biosystems). The CD11a/CD18 cDNA sequences were deduced from sequences obtained from four independent wild boars. Sequences data have been deposited at GenBank under accession nos. EF585976 and EF585977.
Primers design was performed with Netprimer  and Primer 3 . Nucleotidic sequence and identity analyses were carried out using respectively Chromas v.2.21  and BLAST programs . Alignment of amino acids sequences was drawn by GeneDoc v.2.6.002  following the BLOSUM 62 matrix. SignalP v.2.0.b2  and NetNGlyc v.1.0  provided peptide signal and N-glycosylation sites prediction, respectively. The secondary structures were resolved by the GOR secondary structure prediction method version IV .
adjacent to MIDAS
cluster of differentiation
divalent-cation binding motif
epidermal growth factor
intercellular adhesion molecule
lymphocyte function-associated antigen
ligand-induced metal-binding site
metal-ion dependent adhesion site
simian, Wb : wild boar.
Hauck CR: Cell adhesion receptors - signaling capacity and exploitation by bacterial pathogens. Med Microbiol Immunol (Berl). 2002, 191: 55-62. 10.1007/s00430-002-0119-0.
Hynes RO: Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 1992, 69: 11-25. 10.1016/0092-8674(92)90115-S.
Dib K: BETA 2 integrin signaling in leukocytes. Front Biosci. 2000, 5: D438-451. 10.2741/Pathology.
Humphries MJ, Symonds EJ, Mould AP: Mapping functional residues onto integrin crystal structures. Curr Opin Struct Biol. 2003, 13: 236-243. 10.1016/S0959-440X(03)00035-6.
Zecchinon L, Fett T, Vanden Bergh P, Desmecht D: Anatomy of the lymphocyte function-associated antigen-1. Clin Appl Immunol Rev. 2006, 6: 149-172. 10.1016/j.cair.2006.09.002.
Springer TA, Dustin ML, Kishimoto TK, Marlin SD: The lymphocyte function-associated LFA-1, CD2, and LFA-3 molecules: cell adhesion receptors of the immune system. Annu Rev Immunol. 1987, 5: 223-252.
Springer T, Galfre G, Secher DS, Milstein C: Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979, 9: 301-306. 10.1002/eji.1830090410.
Cabanas C, Sanchez-Madrid F: CD11c (leukocyte integrin CR4 alpha subunit). J Biol Regul Homeost Agents. 1999, 13: 134-136.
Van der Vieren M, Le Trong H, Wood CL, Moore PF, St John T, Staunton DE, Gallatin WM: A novel leukointegrin, alpha d beta 2, binds preferentially to ICAM-3. Immunity. 1995, 3: 683-690. 10.1016/1074-7613(95)90058-6.
Danilenko DM, Rossitto PV, Van der Vieren M, Le Trong H, McDonough SP, Affolter VK, Moore PF: A novel canine leukointegrin, alpha d beta 2, is expressed by specific macrophage subpopulations in tissue and a minor CD8+ lymphocyte subpopulation in peripheral blood. J Immunol. 1995, 155: 35-44.
Gahmberg CG, Valmu L, Fagerholm S, Kotovuori P, Ihanus E, Tian L, Pessa-Morikawa T: Leukocyte integrins and inflammation. Cell Mol Life Sci. 1998, 54: 549-555. 10.1007/s000180050183.
Arnaout MA: Leukocyte adhesion molecules deficiency: its structural basis, pathophysiology and implications for modulating the inflammatory response. Immunol Rev. 1990, 114: 145-180. 10.1111/j.1600-065X.1990.tb00564.x.
Trowald-Wigh G, Hakansson L, Johannisson A, Norrgren L, Hard af Segerstad C: Leucocyte adhesion protein deficiency in Irish setter dogs. Vet Immunol Immunopathol. 1992, 32: 261-280. 10.1016/0165-2427(92)90050-Z.
Nagahata H, Kehrli ME, Murata H, Okada H, Noda H, Kociba GJ: Neurtrophil function and pathologic findings in Holstein calves with leukocyte adhesion deficiency. Am J Vet Res. 1994, 55: 40-48.
Hogg N, Smith A, McDowall A, Giles K, Stanley P, Laschinger M, Henderson R: How T cells use LFA-1 to attach and migrate. Immunol Lett. 2004, 92: 51-54. 10.1016/j.imlet.2003.10.014.
Salas A, Shimaoka M, Kogan AN, Harwood C, von Andrian UH, Springer TA: Rolling adhesion through an extended conformation of integrin alphaLbeta2 and relation to alpha I and beta I-like domain interaction. Immunity. 2004, 20: 393-406. 10.1016/S1074-7613(04)00082-2.
Yan SR, Sapru K, Issekutz AC: The CD11/CD18 (beta2) integrins modulate neutrophil caspase activation and survival following TNF-alpha or endotoxin induced transendothelial migration. Immunol Cell Biol. 2004, 82: 435-446. 10.1111/j.0818-9641.2004.01268.x.
Giblin PA, Lemieux RM: LFA-1 as a key regulator of immune function: approaches toward the development of LFA-1-based therapeutics. Curr Pharm Des. 2006, 12: 2771-2795. 10.2174/138161206777947731.
Kellersch B, Kolanus W: Membrane-proximal signaling events in beta-2 integrin activation. Results Probl Cell Differ. 2006, 43: 245-257.
Xingyuan M, Wenyun Z, Tianwen W: Leukocyte function-associated antigen-1: structure, function and application prospects. Protein Pept Lett. 2006, 13: 397-400. 10.2174/092986606775974429.
Zecchinon L, Fett T, Vanden Bergh P, Desmecht D: Bind another day: The LFA-1/ICAM-1 interaction as therapeutic target. Clin Appl Immunol Rev. 2006, 6: 173-189. 10.1016/j.cair.2006.09.004.
Kerr JR: Cell adhesion molecules in the pathogenesis of and host defence against microbial infection. Mol Pathol. 1999, 52: 220-230.
Welch RA, Dellinger EP, Minshew B, Falkow S: Haemolysin contributes to virulence of extra-intestinal E. coli infections. Nature. 1981, 294: 665-667. 10.1038/294665a0.
Welch RA: Pore-forming cytolysins of gram-negative bacteria. Mol Microbiol. 1991, 5: 521-528. 10.1111/j.1365-2958.1991.tb00723.x.
Lally ET, Kieba IR, Sato A, Green CL, Rosenbloom J, Korostoff J, Wang JF, Shenker BJ, Ortlepp S, Robinson MK, Billings PC: RTX toxins recognize a beta2 integrin on the surface of human target cells. J Biol Chem. 1997, 272: 30463-30469. 10.1074/jbc.272.48.30463.
Deshpande MS, Ambagala TC, Ambagala AP, Kehrli ME, Srikumaran S: Bovine CD18 is necessary and sufficient to mediate Mannheimia (Pasteurella) haemolytica leukotoxin-induced cytolysis. Infect Immun. 2002, 70: 5058-5064. 10.1128/IAI.70.9.5058-5068.2002.
Zecchinon L, Fett T, Desmecht D: How Mannheimia haemolytica defeats host defence through a kiss of death mechanism. Vet Res. 2005, 36: 133-156. 10.1051/vetres:2004065.
Bosse JT, Janson H, Sheehan BJ, Beddek AJ, Rycroft AN, Kroll JS, Langford PR: Actinobacillus pleuropneumoniae: pathobiology and pathogenesis of infection. Microbes Infect. 2002, 4: 225-235. 10.1016/S1286-4579(01)01534-9.
Losinger WC: Economic impacts of reduced pork production associated with the diagnosis of Actinobacillus pleuropneumoniae on grower/finisher swine operations in the United States. Prev Vet Med. 2005, 68: 181-193. 10.1016/j.prevetmed.2004.12.004.
Lee JK, Schook LB, Rutherford MS: Molecular cloning and characterization of the porcine CD18 leukocyte adhesion molecule. Xenotransplantation. 1996, 3: 222-230.
Vanden Bergh PG, Fett T, Zecchinon LL, Thomas AV, Desmecht DJ: The CD11a partner in Sus scrofa lymphocyte function-associated antigen-1 (LFA-1): mRNA cloning, structure analysis and comparison with mammalian homologues. BMC Vet Res. 2005, 1: 5-10.1186/1746-6148-1-5.
Vengust G, Valencak Z, Bidovec A: A serological survey of selected pathogens in wild boar in Slovenia. J Vet Med B Infect Dis Vet Public Health. 2006, 53: 24-27.
Springer TA: Adhesion receptors of the immune system. Nature. 1990, 346: 425-434. 10.1038/346425a0.
Colombatti A, Bonaldo P: The superfamily of proteins with von Willebrand factor type A-like domains: one theme common to components of extracellular matrix, hemostasis, cellular adhesion, and defense mechanisms. Blood. 1991, 77: 2305-2315.
Qu A, Leahy DJ: Crystal structure of the I-domain from the CD11a/CD18 (LFA-1, alpha L beta 2) integrin. Proc Natl Acad Sci U S A. 1995, 92: 10277-10281. 10.1073/pnas.92.22.10277.
Edwards CP, Champe M, Gonzalez T, Wessinger ME, Spencer SA, Presta LG, Berman PW, Bodary SC: Identification of amino acids in the CD11a I-domain important for binding of the leukocyte function-associated antigen-1 (LFA-1) to intercellular adhesion molecule-1 (ICAM-1). J Biol Chem. 1995, 270: 12635-12640. 10.1074/jbc.270.21.12635.
Mould AP, Humphries MJ: Regulation of integrin function through conformational complexity: not simply a knee-jerk reaction?. Curr Opin Cell Biol. 2004, 16: 544-551. 10.1016/j.ceb.2004.07.003.
Larson RS, Corbi AL, Berman L, Springer T: Primary structure of the leukocyte function-associated molecule-1 alpha subunit: an integrin with an embedded domain defining a protein superfamily. J Cell Biol. 1989, 108: 703-712. 10.1083/jcb.108.2.703.
Beglova N, Blacklow SC, Takagi J, Springer TA: Cysteine-rich module structure reveals a fulcrum for integrin rearrangement upon activation. Nat Struct Biol. 2002, 9: 282-287. 10.1038/nsb779.
Rothlein R, Springer TA: The requirement for lymphocyte function-associated antigen 1 in homotypic leukocyte adhesion stimulated by phorbol ester. J Exp Med. 1986, 163: 1132-1149. 10.1084/jem.163.5.1132.
Xie C, Shimaoka M, Xiao T, Schwab P, Klickstein LB, Springer TA: The integrin alpha-subunit leg extends at a Ca2+-dependent epitope in the thigh/genu interface upon activation. Proc Natl Acad Sci U S A. 2004, 101: 15422-15427. 10.1073/pnas.0406680101.
Pardi R, Bossi G, Inverardi L, Rovida E, Bender JR: Conserved regions in the cytoplasmic domains of the leukocyte integrin alpha L beta 2 are involved in endoplasmic reticulum retention, dimerization, and cytoskeletal association. J Immunol. 1995, 155: 1252-1263.
Loftus BJ, Kim UJ, Sneddon VP, Kalush F, Brandon R, Fuhrmann J, Mason T, Crosby ML, Barnstead M, Cronin L, Deslattes Mays A, Cao Y, Xu RX, Kang HL, Mitchell S, Eichler EE, Harris PC, Venter JC, Adams MD: Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q. Genomics. 1999, 60: 295-308. 10.1006/geno.1999.5927.
Walter NA, Stebbing J, Messier W: The potential significance of adaptive evolution and dimerization in chimpanzee intercellular cell adhesion molecules (ICAMs). J Theor Biol. 2005, 232: 339-346.
Fett T, Zecchinon L, Baise E, Desmecht D: Cloning and characterisation of the primary structure of the sheep lymphocyte function-associated antigen-1 alpha subunit. Mol Immunol. 2005, 42: 1503-1508. 10.1016/j.molimm.2005.01.005.
Fett T, Zecchinon L, Baise E, Desmecht D: The bovine (Bos taurus) CD11a-encoding cDNA: molecular cloning, characterisation and comparison with the human and murine glycoproteins. Gene. 2004, 325: 97-101. 10.1016/j.gene.2003.09.043.
Fett T, Zecchinon LL, Baise EA, Desmecht DJ: Molecular characterisation of the caprine (Capra hircus) lymphocyte function-associated antigen-1 alpha subunit-encoding cDNA. BMC Vet Res. 2005, 1: 4-10.1186/1746-6148-1-4.
Kaufmann Y, Tseng E, Springer TA: Cloning of the murine lymphocyte function-associated molecule-1 alpha-subunit and its expression in COS cells. J Immunol. 1991, 147: 369-374.
Dustin ML, Springer TA: T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1. Nature. 1989, 341: 619-624. 10.1038/341619a0.
Vitte J, Pierres A, Benoliel AM, Bongrand P: Direct quantification of the modulation of interaction between cell- or surface-bound LFA-1 and ICAM-1. J Leukoc Biol. 2004, 76: 594-602. 10.1189/jlb.0204077.
Kim M, Carman CV, Springer TA: Bidirectional transmembrane signaling by cytoplasmic domain separation in integrins. Science. 2003, 301: 1720-1725. 10.1126/science.1084174.
Hibbs ML, Xu H, Stacker SA, Springer TA: Regulation of adhesion of ICAM-1 by the cytoplasmic domain of LFA-1 integrin beta subunit. Science. 1991, 251: 1611-1613. 10.1126/science.1672776.
van Kooyk Y, Binnerts ME, Edwards CP, Champe M, Berman PW, Figdor CG, Bodary SC: Critical amino acids in the lymphocyte function-associated antigen-1 I domain mediate intercellular adhesion molecule 3 binding and immune function. J Exp Med. 1996, 183: 1247-1252. 10.1084/jem.183.3.1247.
Bork P, Doerks T, Springer TA, Snel B: Domains in plexins: links to integrins and transcription factors. Trends Biochem Sci. 1999, 24: 261-263. 10.1016/S0968-0004(99)01416-4.
Shi M, Sundramurthy K, Liu B, Tan SM, Law SK, Lescar J: The crystal structure of the plexin-semaphorin-integrin domain/hybrid domain/I-EGF1 segment from the human integrin beta2 subunit at 1.8-A resolution. J Biol Chem. 2005, 280: 30586-30593. 10.1074/jbc.M502525200.
Huang C, Zang Q, Takagi J, Springer TA: Structural and functional studies with antibodies to the integrin beta 2 subunit. A model for the I-like domain. J Biol Chem. 2000, 275: 21514-21524. 10.1074/jbc.M002286200.
Takagi J, Beglova N, Yalamanchili P, Blacklow SC, Springer TA: Definition of EGF-like, closely interacting modules that bear activation epitopes in integrin beta subunits. Proc Natl Acad Sci U S A. 2001, 98: 11175-11180. 10.1073/pnas.201420198.
Chen J, Yang W, Kim M, Carman CV, Springer TA: Regulation of outside-in signaling and affinity by the beta2 I domain of integrin alphaLbeta2. Proc Natl Acad Sci U S A. 2006, 103: 13062-13067. 10.1073/pnas.0605666103.
Bleijs DA, van Duijnhoven GC, van Vliet SJ, Thijssen JP, Figdor CG, van Kooyk Y: A single amino acid in the cytoplasmic domain of the beta 2 integrin lymphocyte function-associated antigen-1 regulates avidity-dependent inside-out signaling. J Biol Chem. 2001, 276: 10338-10346. 10.1074/jbc.M008967200.
Shuster DE, Bosworth BT, Kehrli ME: Sequence of the bovine CD18-encoding cDNA: comparison with the human and murine glycoproteins. Gene. 1992, 114: 267-271. 10.1016/0378-1119(92)90586-E.
Zecchinon L, Fett T, Baise E, Desmecht D: Characterization of the caprine (Capra hircus) beta-2 integrin CD18-encoding cDNA and identification of mutations potentially responsible for the ruminant-specific virulence of Mannheimia haemolytica. Mol Membr Biol. 2004, 21: 289-295. 10.1080/09687680412331282785.
Zecchinon L, Fett T, Baise E, Desmecht D: Molecular cloning and characterisation of the CD18 partner in ovine (Ovis aries) beta2-integrins. Gene. 2004, 334: 47-52. 10.1016/j.gene.2004.03.011.
Liu W, Brayton KA, Lagerquist J, Foreyt WJ, Srikumaran S: Cloning and comparison of bighorn sheep CD18 with that of domestic sheep, goats, cattle, humans and mice. Vet Immunol Immunopathol. 2006, 110: 11-16. 10.1016/j.vetimm.2005.08.030.
Weitzman JB, Wells CE, Wright AH, Clark PA, Law SK: The gene organisation of the human beta 2 integrin subunit (CD18). FEBS Lett. 1991, 294: 97-103. 10.1016/0014-5793(91)81351-8.
Kijas JM, Bauer TR, Gafvert S, Marklund S, Trowald-Wigh G, Johannisson A, Hedhammar A, Binns M, Juneja RK, Hickstein DD, Andersson L: A missense mutation in the beta-2 integrin gene (ITGB2) causes canine leukocyte adhesion deficiency. Genomics. 1999, 61: 101-107. 10.1006/geno.1999.5948.
Wilson RW, O'Brien WE, Beaudet AL: Nucleotide sequence of the cDNA from the mouse leukocyte adhesion protein CD18. Nucleic Acids Res. 1989, 17: 5397-10.1093/nar/17.13.5397.
Bilsland CA, Springer TA: Cloning and expression of the chicken CD18 cDNA. J Leukoc Biol. 1994, 55: 501-506.
Qian Y, Ainsworth AJ, Noya M: Identification of a beta 2 (CD18) molecule in a teleost species, Ictalurus punctatus Rafinesque. Dev Comp Immunol. 1999, 23: 571-583. 10.1016/S0145-305X(99)00040-3.
Kamata T, Tieu KK, Tarui T, Puzon-McLaughlin W, Hogg N, Takada Y: The role of the CPNKEKEC sequence in the beta(2) subunit I domain in regulation of integrin alpha(L)beta(2) (LFA-1). J Immunol. 2002, 168: 2296-2301.
Chen J, Salas A, Springer TA: Bistable regulation of integrin adhesiveness by a bipolar metal ion cluster. Nat Struct Biol. 2003, 10: 995-1001. 10.1038/nsb1011.
Mould AP, Barton SJ, Askari JA, Craig SE, Humphries MJ: Role of ADMIDAS cation-binding site in ligand recognition by integrin alpha 5 beta 1. J Biol Chem. 2003, 278: 51622-51629. 10.1074/jbc.M306655200.
Chen J, Takagi J, Xie C, Xiao T, Luo BH, Springer TA: The relative influence of metal ion binding sites in the I-like domain and the interface with the hybrid domain on rolling and firm adhesion by integrin alpha4beta7. J Biol Chem. 2004, 279: 55556-55561. 10.1074/jbc.M407773200.
Chatila TA, Geha RS, Arnaout MA: Constitutive and stimulus-induced phosphorylation of CD11/CD18 leukocyte adhesion molecules. J Cell Biol. 1989, 109: 3435-3444. 10.1083/jcb.109.6.3435.
Fagerholm SC, Hilden TJ, Nurmi SM, Gahmberg CG: Specific integrin alpha and beta chain phosphorylations regulate LFA-1 activation through affinity-dependent and -independent mechanisms. J Cell Biol. 2005, 171: 705-715. 10.1083/jcb.200504016.
Rozen S, Skaletsky HJ: Primer3 on the WWW for general users and for biologist programmers. Bioinformatics Methods and Protocols: Methods in Molecular Biology. Totowa, Krawetz S., Misener S.Humana Press;2000:365-386.
Chromas v2.21. [[http://www.technelysium.com.au]]
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol. 1990, 215: 403-410.
Nicholas B, Karl B, Nicholas P, Hugh BJ: GeneDoc : a tool for editing and annotating multiple sequence alignements. [[http://www.psc.edu/biomed/genedoc]]
Nielsen H, Engelbrecht J, Brunak S, von Heijne G: Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 1997, 10: 1-6. 10.1093/protein/10.1.1.
Jensen LJ, Gupta R, Blom N, Devos D, Tamames J, Kesmir C, Nielsen H, Staerfeldt HH, Rapacki K, Workman C, Andersen CA, Knudsen S, Krogh A, Valencia A, Brunak S: Prediction of human protein function from post-translational modifications and localization features. J Mol Biol. 2002, 319: 1257-1265. 10.1016/S0022-2836(02)00379-0.
Garnier J, Gibrat JF, Robson B: GOR method for predicting protein secondary structure from amino acid sequence. Methods Enzymol. 1996, 266: 540-553.
Philippe Vanden Bergh is the recipient of a studentship from the "Fonds pour la formation à la Recherche dans l'Industrie et l'Agriculture", rue d'Egmont 5, B-1000 Bruxelles.
PVB carried out amplification, sequencing, sequences alignment and drafting of the manuscript. TF and LZ participated in the design of the study and helped to structure analysis. DD participated in the design of the study and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
About this article
Cite this article
Bergh, P.G.V., Zecchinon, L.L., Fett, T. et al. The wild boar (Sus scrofa) Lymphocyte function-associated antigen-1 (CD11a/CD18) receptor: cDNA sequencing, structure analysis and comparison with homologues. BMC Vet Res 3, 27 (2007). https://doi.org/10.1186/1746-6148-3-27