Fig. 5
From: Transcriptome analysis of PK-15 cells expressing CSFV NS4A
Validation of the RNA-seq data by RT‒qPCR. To confirm the results from RNA-seq, RT‒qPCR was performed using total RNA from NS4A- or NC-expressing PK-15 cells. β-Actin was used as a reference. The y-axis represents the normalized fold changes in the expression of these selected genes. The error bars represent the mean ± SD (n = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.001