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Table 1 Proteins identified in the tear of healthy and infected cats

From: Protein expression of the tear film of domestic cats before and after inoculation with Toxoplasma gondii

Compound name SWISS-PROT ID Mass (Da) Score/Peptide matches
D0a D0b D0c D5a D5b D5c D21a D21b D21c
Allergen Fel d 4 OS=Felis catus PE = 1 SV = 1 ALL4_FELCA 21,580 11,292/346 14,539/383 12,265/350 12,427/316 6378/186 1968/92 9444/ 245 11,693/283 11,127/302
Serum albumin OS=Felis catus GN = ALB PE = 1 SV = 1 ALBU_FELCA 70,611 2740/76 4467/119 3544/93 2867/71 2245/61 933/30 3460/89 3646/93 2601/65
Aldehyde dehydrogenase, dimeric NADP-preferring OS=Canis lupus familiaris GN = ALDH3A1 PE = 2 AL3A1_CANLF 51,193 509/17 731/23 598/17 779/26 647/16 266/7 842/23 775/24 606/20
Lactoperoxidase OS=Bos taurus GN = LPO PE = 1 PERL_BOVIN 81,504 427/18 783/28 562/19 1031/36 535/17 299/9 586/23 684/23 585/21
Lactotransferrin OS=Bos taurus GN = LTF PE = 1 SV = 2 TRFL_BOVIN 80,002 410/15 667/27 534/19 643/20 604/23 283/18 542/18 699/21 450/17
Lactotransferrin (Fragment) OS = Equus caballus GN = LTF PE = 1 SV = 1 TRFL_HORSE 77,938 184/7 238/11 283/12 275/8 425/14 189/6 237/9 343/11 184/8
Inhibitor of carbonic anhydrase OS=Sus scrofa GN=ICA PE = 1 ICA_PIG 79,636 406/10 471/10 375/6 877/17 911/20 187/4 695/15 777/16 514/10
Major allergen I polypeptide chain 1 OS=Felis catus GN=CH1 PE = 1 SV = 2 FEL1A_FELCA 10,364 353/7 781/12 709/9 725/13 ND ND 219/3 746/11 487/9
Trypsin OS=Sus scrofa PE = 1 SV = 1 TRYP_PIG 25,078 255/7 260/11 289/8 459/13 196/8 184/6 255/8 527/12 212/6
Actin, plasmodial isoform OS=Physarum polycephalum GN = ARDA PE = 1 ACTA_PHYPO 42,001 227/10 ND ND ND ND ND ND ND ND
Deleted in malignant brain tumors 1 protein OS=Oryctolagus cuniculus GN=Dmbt1 PE = 1 DMBT1_RABIT 177,328 201/4 293/5 259/4 504/9 327/6 276/7 393/7 167/3 ND
Haptoglobin OS = Ateles geoffroyi GN=HP PE = 2 HPT_ATEGE 38,964 200/11 ND ND ND ND ND ND ND ND
Haptoglobin OS=Canis lupus familiaris GN=HP PE = 1 HPT_CANLF 36,890 128/7 198/7 122/3 265/10 129/5 103/5 218/9 217/23 106/7
Glutathione S-transferase P OS=Bos taurus GN = GSTP1 PE = 1 GSTP1_BOVIN 23,826 190/4 303/6 375/7 407/5 116/2 ND 289/4 573/8 282/4
Ig kappa chain V region GOM OS=Canis lupus familiaris PE = 1 SV = 1 KV1_CANLF 12,112 158/2 428/6 178/2 ND ND ND 205/3 429/5 324/4
Ig lambda chain C region OS=Sus scrofa PE = 1 LAC_PIG 11,168 155/8 435/18 361/15 105/4 ND ND 241/12 421/16 347/11
Polymeric immunoglobulin receptor OS=Bos taurus GN=PIGR PE = 2 PIGR_BOVIN 83,695 150/6 126/6 118/5 ND 106/6 ND 224/12 239/8 ND
Ovostatin homolog 2 OS=Homo sapiens GN=OVOS2 PE = 2 SV = 2 OVOS2_HUMAN 162,517 135/5 271/9 208/6 329/12 316/14 302/15 216/8 226/9 212/8
Retinal dehydrogenase 1 OS=Oryctolagus cuniculus GN = ALDH1A1 PE = 1 AL1A1_RABIT 54,933 110/2 ND ND ND ND ND ND ND 142/4
Ovostatin homolog OS = Mus musculus GN=Ovos PE = 2 SV = 2 OVOS_MOUSE 163,607 108/6 163/11 ND 161/9 215/11 208/11 150/9 162/10 124/7
Immunoglobulin heavy variable 3–15 OS=Homo sapiens GN=IGHV3–15 PE = 3 HV315_HUMAN 13,089 105/4 239/12 112/7 115/4 178/6 102/4 214/8 ND 108/5
Glutathione S-transferase P OS=Bos taurus GN = GSTP1 PE = 1 GSTP1_CRILO 23,851 102/2 198/5 155/4 ND 160/4 ND 174/4 170/4 ND
Lactotransferrin OS=Camelus dromedarius GN = LTF PE = 1 TRFL_CAMDR 79,158 ND 608/22 509/17 725/19 ND ND ND 746/23 ND
Actin-10 OS=Dyctiostelium discoideum GN = act10 PE = 1 SV = 1 ACT10_DICDI 41,948 ND 312/15 ND ND ND ND ND 342/23 368/19
Actin, muscle-type OS = Molgula oculata PE = 3 SV = 1 ACT2_MOLOC 42,521 ND 274/13 ND ND ND ND 487/17 ND ND
Aldehyde dehydrogenase, cytosolic 2 (Fragment) OS = Macroscelides proboscideus PE = 2 ALDH2_MACPR 26,841 ND 274/10 370/9 ND ND ND ND 207/5 ND
Haptoglobin OS=Bos taurus GN=HP PE = 2 SV = 1 HPT_BOVIN 45,629 ND 231/12 158/7 ND 288/17 253/14 186/12 180/10 118/10
Selenium-binding protein 1 OS = Rattus norvegicus GN=Selenbp1 PE = 1 SV = 1 SBP1_RAT 53,069 ND 256/9 ND 177/8 ND ND 297/11 ND ND
Alpha-enolase OS=Homo sapiens GN = ENO1 PE = 1 SV = 2 ENOA_HUMAN 47,481 ND 220/8 179/5 173/7 ND ND 163/6 151/6 177/6
14–3-3 protein sigma OS=Bos taurus GN=SFN PE = 2 1433S_BOVIN 27,946 ND 133/10 119/3 172/6 140/7 ND 325/14 236/12 194/7
Malate dehydrogenase OS = Myxococcus xanthus GN = mdh PE = 3 SV = 1 MDH_MYXXA 33,194 ND 120/14 ND 101/16 ND ND ND 106/19 118/14
Serotransferrin OS = Mus musculus GN = Tf PE = 1 SV = 1 TRFE_MOUSE 78,841 ND 109/6 ND 108/6 141/7 134/7 133/6 105/6 ND
14–3-3 protein zeta/delta OS=Bos taurus GN=YWHAZ PE = 1 SV = 1 1433Z_BOVIN 27,899 ND ND 104/3 ND ND ND ND ND ND
Actin, muscle OS = Manduca sexta PE = 2 SV = 1 ACT_MANSE 42,149 ND ND 213/15 559/15 ND ND ND ND ND
Selenium-binding protein 1 OS=Homo sapiens GN=SELENBP1 PE = 1 SBP1_HUMAN 52,928 ND ND 160/4 ND 113/3 108/3 ND 232/10 142/4
Retinal dehydrogenase 1 OS=Homo sapiens GN = ALDH1A1 PE = 1 SV = 2 AL1A1_HUMAN 55,454 ND ND 254/6 ND ND ND ND 121/3 ND
Lactotransferrin OS=Sus scrofa GN = LTF PE = 1 SV = 3 TRFL_PIG 79,514 ND ND 126/7 ND ND ND 111/7 103/5 ND
Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE = 1 K2C1_HUMAN 66,170 ND ND ND 296/12 298/9 ND ND ND ND
Phosphoglycerate mutase 1 OS=Bos taurus GN=PGAM1 PE = 2 PGAM1_BOVIN 28,948 ND ND ND 113/4 ND ND ND ND ND
Actin, cytoplasmic 1 OS=Bos mutus grunniens GN = ACTB PE = 2 ACTB_BOSMU 42,064 ND ND ND ND 248/18 ND ND ND ND
Elongation factor 1-alpha, somatic form OS = Xenopus laevis GN = eef1as PE = 2 EF1A0_XENLA 50,524 ND ND ND ND 142/6 ND ND ND ND
Retinal dehydrogenase 1 OS=Bos taurus GN = ALDH1A1 PE = 1 SV = 3 AL1A1_BOVIN 55,398 ND ND ND ND 128/2 ND ND ND ND
Lactoperoxidase OS=Homo sapiens GN = LPO PE = 1 SV = 2 PERL_HUMAN 81,149 ND ND ND ND 341/11 424/13 ND ND ND
Actin-17 OS=Dictyostelium discoideum GN = act17 PE = 3 ACT17_DICDI 41,773 ND ND ND ND ND 177/10 ND ND ND
Elongation factor 1-alpha OS=Blastobotrys adeninivorans GN = TEF PE = 3 EF1A_BLAAD 50,426 ND ND ND ND ND 115/4 ND ND ND
Polymeric immunoglobulin receptor OS=Homo sapiens GN=PIGR PE = 1 PIGR_HUMAN 84,429 ND ND ND ND ND 105/3 109/6 ND ND
Actin-1 OS = Aedes aegypti GN = ACT-1 PE = 2 SV = 2 ACT1_AEDAE 42,045 ND ND ND ND ND ND 490/17 361/20 335/17
Keratin, type II cytoskeletal 6A OS = Rattus norvegicus GN=Krt6a PE = 1 SV = 1 K2C6A_RAT 59,555 ND ND ND ND ND ND 331/7 123/3 ND
Ceruloplasmin OS=Homo sapiens GN=CP PE = 1 CERU_HUMAN 122,983 ND ND ND ND ND ND 196/6 188/8 124/3
Alpha-enolase OS=Bos taurus GN = ENO1 PE = 1 SV = 4 ENOA_BOVIN 47,639 ND ND ND ND ND ND 163/7 171/6 ND
Gelsolin OS=Homo sapiens GN = GSN PE = 1 SV = 1 GELS_HUMAN 86,043 ND ND ND ND ND ND 148/6 186/8 122/5
Elongation factor 1-alpha OS=Onchocerca volvulus PE = 2 EF1A_ONCVO 51,090 ND ND ND ND ND ND 106/3 ND ND
Alpha-crystallin B chain OS=Bos taurus GN=CRYAB PE = 1 CRYAB_BOVIN 20,024 ND ND ND ND ND ND ND 140/3 ND
Annexin A1 (Fragment) OS = Gallus gallus GN = ANXA1 PE = 2 SV = 1 ANXA1_CHICK 14,446 ND ND ND ND ND ND ND 121/2 ND
  1. ND not detected; or score less than 100
  2. These proteins were identified in the tear of healthy cats, before infection with T. gondii cysts, 5 days and 21 days later, analyzed in triplicate (a, b and c)