Fig. 1

Thrombin generation curves obtained by the calibrated automated thrombogram (CAT) assay for serial dilutions of unfractionated heparin in pooled equine citrated plasma. Thrombin generation decreased with increasing concentrations of unfractionated heparin. Heparin concentrations above 0.18 U/mL completely inhibited thrombin generation and produced a flat-line (0 nM thrombin) and 0.37 U/mL, 0.75 U/mL, 1.5 U/mL and 3 U/mL are therefore not visible on the graph. A heparin concentration of 0.01125 U/ml and 0.0225 U/ml produced a slightly higher peak and endogenous thrombin potential than heparin concentration of 0.0 U/mL