Fig. 1

Schematic diagram of in vitro Maturation protocol, starting from superovulation via PMSG and hCG 150 IU 48 and 16-h prior sample collection respectively. Ovaries are collected via surgical incision and then dissected in M2 washing media, oocytes with 3 layers of intact cumulus cells are selected. They then incubated for 20 h in Co-culture or simple culture containing culture media TCM 199 supplemented with Fetal calf serum 10% volume by volume, estrogen 1 μg/ml, EGF 10 ng/ml, Cysteamine 100uM, Sodium pyruvate 0.45 mM, FSH 5 μg/ml. Figure is created by author himself using miscrsoft power point software and own all rights to it