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Table 4 Effects of Flemingia macrophylla silage (FMS) on nitrogen balance, excretion of purine derivatives and microbial nitrogen supply

From: Strategic supplementation of Flemingia silage to enhance rumen fermentation efficiency, microbial protein synthesis and methane mitigation in beef cattle

Items

FMS kg/d of dry mater

SEM

P-value

0

0.2

0.4

0.6

Nitrogen utilization (g/d)

 Intake

34.81 ± 1.23a

41.02 ± 1.54b

44.03 ± 1.98c

48.01 ± 2.12d

2.79

0.001

N excretion (g/d)

 Fecal N

15.20 ± 2.32

16.00 ± 1.98

16.20 ± 1.67

13.90 ± 1.59

0.52

0.54

 Urinal N

13.00 ± 1.41

13.40 ± 1.50

11.80 ± 1.83

10.50 ± 1.92

0.65

0.25

N balance (g/d)

 Absorbed N

19.61 ± 1.04a

25.02 ± 1.67b

28.01 ± 1.91b

34.10 ± 1.98c

3.02

0.001

 Retained N

6.70 ± 0.54a

11.61 ± 0.946b

17.63 ± 1.32c

22.30 ± 1.27d

3.41

0.001

Allantoin (mM/d)

108.92 ± 3.54a

122.61 ± 4.21b

130.67 ± 5.33b

148.71 ± 8.12c

8.30

0.001

Uric acid (mM/d)

26.11 ± 1.34a

29.42 ± 2.30b

31.33 ± 1.11b

35.56 ± 1.72c

1.99

0.001

PD (mM/d)

130.67 ± 3.43a

147.22 ± 2.31b

156.78 ± 3.16b

178.43 ± 2.81c

9.96

0.001

Purine absorb (mM/d)

109.3 ± 2.43a

125.7 ± 2.56b

135.4 ± 2.11b

157.0 ± 2.43c

9.96

0.001

MNS (g N/d)

79.41 ± 2.21a

91.4 ± 3.11b

98.4 ± 4.01b

114.1 ± 4.87c

7.24

0.001

MNS increased (%)

0.00 ± 0.00a

15.43 ± 1.65b

24.12 ± 1.93b

44.01 ± 2.88c

9.20

0.002

EMNS (g N/kg OMDR)

9.60 ± 0.13a

11.31 ± 0.46b

14.13 ± 0.72c

14.12 ± 0.54c

1.01

0.003

EMNS increased (%)

0.00 ± 0.00a

18.92 ± 1.63b

39.78 ± 1.97b

49.01 ± 2.41c

10.9

0.001

  1. a,b,c Means in the same row with different superscripts differ (P < 0.05), SEM Standard error of the mean, N Nitrogen, MNS Microbial nitrogen supply, PD Purine derivatives. Microbial N (g N/d) = (X×70)/(0.116×0.83×1,000) = 0.727×X (where, X = total absorption of purine derivatives). EMNS = Efficiency of microbial nitrogen supply (g N/kg OMDR). OMDR (kg) = 65% of organic matter digestible in total tract