Skip to main content

Table 4 Effects of Flemingia macrophylla silage (FMS) on nitrogen balance, excretion of purine derivatives and microbial nitrogen supply

From: Strategic supplementation of Flemingia silage to enhance rumen fermentation efficiency, microbial protein synthesis and methane mitigation in beef cattle

Items FMS kg/d of dry mater SEM P-value
0 0.2 0.4 0.6
Nitrogen utilization (g/d)
 Intake 34.81 ± 1.23a 41.02 ± 1.54b 44.03 ± 1.98c 48.01 ± 2.12d 2.79 0.001
N excretion (g/d)
 Fecal N 15.20 ± 2.32 16.00 ± 1.98 16.20 ± 1.67 13.90 ± 1.59 0.52 0.54
 Urinal N 13.00 ± 1.41 13.40 ± 1.50 11.80 ± 1.83 10.50 ± 1.92 0.65 0.25
N balance (g/d)
 Absorbed N 19.61 ± 1.04a 25.02 ± 1.67b 28.01 ± 1.91b 34.10 ± 1.98c 3.02 0.001
 Retained N 6.70 ± 0.54a 11.61 ± 0.946b 17.63 ± 1.32c 22.30 ± 1.27d 3.41 0.001
Allantoin (mM/d) 108.92 ± 3.54a 122.61 ± 4.21b 130.67 ± 5.33b 148.71 ± 8.12c 8.30 0.001
Uric acid (mM/d) 26.11 ± 1.34a 29.42 ± 2.30b 31.33 ± 1.11b 35.56 ± 1.72c 1.99 0.001
PD (mM/d) 130.67 ± 3.43a 147.22 ± 2.31b 156.78 ± 3.16b 178.43 ± 2.81c 9.96 0.001
Purine absorb (mM/d) 109.3 ± 2.43a 125.7 ± 2.56b 135.4 ± 2.11b 157.0 ± 2.43c 9.96 0.001
MNS (g N/d) 79.41 ± 2.21a 91.4 ± 3.11b 98.4 ± 4.01b 114.1 ± 4.87c 7.24 0.001
MNS increased (%) 0.00 ± 0.00a 15.43 ± 1.65b 24.12 ± 1.93b 44.01 ± 2.88c 9.20 0.002
EMNS (g N/kg OMDR) 9.60 ± 0.13a 11.31 ± 0.46b 14.13 ± 0.72c 14.12 ± 0.54c 1.01 0.003
EMNS increased (%) 0.00 ± 0.00a 18.92 ± 1.63b 39.78 ± 1.97b 49.01 ± 2.41c 10.9 0.001
  1. a,b,c Means in the same row with different superscripts differ (P < 0.05), SEM Standard error of the mean, N Nitrogen, MNS Microbial nitrogen supply, PD Purine derivatives. Microbial N (g N/d) = (X×70)/(0.116×0.83×1,000) = 0.727×X (where, X = total absorption of purine derivatives). EMNS = Efficiency of microbial nitrogen supply (g N/kg OMDR). OMDR (kg) = 65% of organic matter digestible in total tract