|
Items
|
FMS kg/d of dry mater
|
SEM
|
P-value
|
|---|
|
0
|
0.2
|
0.4
|
0.6
|
|---|
|
NH3-N (mg/ml)
|
19.31 ± 0.12d
|
18.32 ± 0.13c
|
17.53 ± 0.11b
|
16.91 ± 0.08a
|
0.50
|
0.001
|
|
BUN (mg/dl)
|
10.11 ± 0.13
|
10.03 ± 0.21
|
10.21 ± 0.43
|
10.03 ± 0.38
|
0.02
|
0.83
|
|
Total VFA (mM/L)
|
99.50 ± 4.61a
|
102.90 ± 4.61ab
|
107.70 ± 3.52bc
|
112.50 ± 4.23c
|
2.82
|
0.004
|
|
VFA (mol/100 mol)
|
|
Acetic acid
|
73.90 ± 0.67d
|
73.00 ± 0.36c
|
71.70 ± 0.94b
|
70.20 ± 0.98a
|
0.81
|
0.001
|
|
Propionic acid
|
16.50 ± 0.20a
|
17.70 ± 0.19b
|
19.30 ± 0.59c
|
20.90 ± 0.74d
|
0.95
|
0.001
|
|
Butyric acid
|
9.70 ± 0.53c
|
9.20 ± 0.33b
|
9.00 ± 0.39ab
|
8.90 ± 0.26a
|
0.18
|
0.002
|
|
Acetic acid to Propionic acid
|
4.50 ± 0.08d
|
4.10 ± 0.06c
|
3.80 ± 0.16b
|
3.40 ± 0.16a
|
0.23
|
0.001
|
|
Methane (mol/100 mol)
|
32.60 ± 0.16d
|
31.68 ± 0.14c
|
30.59 ± 0.45b
|
29.38 ± 0.55a
|
0.69
|
0.001
|
|
pH
|
6.82 ± 0.03
|
6.87 ± 0.02
|
6.84 ± 0.03
|
6.89 ± 0.02
|
0.02
|
0.13
|
|
Protozoa (×105Cell/ml)
|
8.08 ± 0.07c
|
6.65 ± 0.05b
|
4.98 ± 0.07a
|
4.40 ± 0.09a
|
0.83
|
0.009
|
- a,b,c,dMeans in the same row with different superscripts differ (P < 0.01), SEM Standard error of the mean, BUN Blood urea nitrogen, VFA Volatile fatty acid, Methane production=0.45 (acetic acid) – 0.275 (propionic acid) + 0.4 (butyric acid)
