Supranutritional selenium level minimizes high concentrate diet-induced epithelial injury by alleviating oxidative stress and apoptosis in colon of goat

Table 4 Primers for real time RT-PCR

Gene	Primer sequence 5’ to 3’	Accession number	Size(bp)
GAPDH	GGGTCATCATCTCTGCACCT	HM043737.1	180
	GGTCATAAGTCCCTCCACGA
Bcl2	TCGCCCAAGTCAAACATTA	AY423861.1	208
	CACAGGTGAAACTGCCAAGAT
Bax	TGCTCACTGCCTCACTCAC	AF163774.1	178
	CCAAGACCACTCCTCCCTA
Caspase3	GGTTCATCCAGGCTCTTT	AF068837.1	98
	TTCTGTCGCTACCTTTCG
Caspase8	GGCTCCTCTGAGATGCTG	NM-001045970	149
	TGCTCCCGTGCTATGCTA

GAPDH mRNA, Glyceraldehyde 3-phosphate dehydrogenase ribosomal RNA; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated x protein; Caspase, cysteine-aspartic proteases. The first primer listed for each gene is the forward primer and the second primer is reverse primer

ISSN: 1746-6148