Fig. 2

The comparison of histological damages in colonic epithelium of LC, HC and HC-SY. Goats were fed experimental diets for 10 weeks with total 0.15, 0.15 and 0.65 mg.kg^− 1 diet Se concentration in LC, HC and HC-SY respectively. Colon (n = 6) from each group were processed for histological evaluation: colon section of LC group (a, scale bar = 100 µm); HC group (b, scale bar = 100 µm) and HC-SY (c, scale bar = 100 µm) at 10X magnification. Representative histological sections of the colon tissue were stained by H&E. Colon injury scoring was categorized on the basis of histological damages indicated by black, yellow and red arrow showing epithelial injury score (d), goblet cell depletion (e) and inflammatory cell infiltration (f) respectively. Values are means ± SEM. The significance was considered at P < 0.05