Seroprevalence of and risk factors for Q fever in dairy and slaughterhouse cattle of Jimma town, South Western Ethiopia

Abstract

Background

Q fever is a zoonotic disease, caused by Gram negative bacterium C. burnetii, which imparts significant socio-economic burden due to production and reproductive loss (abortion, stillbirth, and infertility) in ruminants and debilitating clinical disease in human populations. While sheep and goats are considered the primary reservoirs of infection to humans, infection can also result from exposure to cattle. Recent studies indicate that in Ethiopia Q fever is a disease of growing public health interest. The top cattle producing region in Ethiopia is the Oromia region and Jimma is the zone that ranks first in the population of cattle within Oromia. While in Jimma zone livestock production plays an important role in people’s livelihoods and nutrition, to date, there is no available report on seroprevalence of Q fever in cattle. This is particularly important due to the low dairy farm biosecurity in Jimma town. This study aimed to evaluate the potential risk for public health from cattle production; a specific objective of this study included the estimation of the seroprevalence of C. burnetii infection and its potential risk factors in dairy cattle and cattle for slaughter in Jimma Town.

Results

The seroprevalence of C. burnetii in cattle present at dairy farms was significantly lower compared to cattle presented at slaughterhouse [6.17% (95% CI: 3.41–10.13) and 11.79% (95% CI: 7.63–17.17), respectively; (P = 0.04)]. As the age of dairy cattle increase by 1 year, they were 1.51 more likely to be positive of C. burnetii [OR = 1.51 (95%CI: 1.30–1.75; (P = 0.000)]. Cattle managed in semi-intensive production systems were 8.08 more likely to be C. burnetii seropositive compared to intensively managed dairy cattle [OR = 8.08 (95%CI: 1.03–63.68); P = 0.047]. Dairy cattle with access to nuisance animals like dogs, cats and mice were 5.65 more likely to be C. burnetii seropositive compared to dairy cattle without access to these animals. On the other hand, dairy cattle that have no tick infestation are 93% less likely to be seropositive for C. burnetii [OR = 0.07 (95%CI: 0.01–0.74); P = 0.027]. Concerning farm-level data, farms of larger herd sizes were 1.03 more likely to be C. burnetii seropositive than small herd farms [OR = 1.03 (95%CI: 0.99–1.06)]. The result from slaughterhouse indicates that as the age of cattle increase by 1 year their chance of being C. burnetii seropositive increases by 2.27 [OR = 2.27 (95%CI: 1.93–2.68); p = 0.000].

Conclusion

Considering its zoonotic and economic burden the seroprevalence of Q fever recorded in this study is of eminent public health concern with a farm-level and slaughterhouse seroprevalence of 6.17 and 11.79% respectively. Based on modifiable risk factors identified in this study, Q fever management plans better be focused on health education and awareness campaigns for abattoir workers and dairy farm workers. Dairy farm Q fever management plans should contemplate improved dairy herd biosecurity with regards to cattle tick infestation, keeping different livestock species segregated and avoiding mixing of herd with others with unknown health status.

Background

Q fever is caused by highly infectious, ubiquitous and pleomorphic intracellular Gram-negative bacterium name C. burnetii. The organism can persist in a spore-like form for more than 40 months [11, 40]. The disease is classified as an emerging zoonotic infectious disease according to WHO, FAO, OIE and EFSA/ECDC [3, 15]. Sheep and goats are considered to be the major sources of human outbreaks due to Coxiella, but cattle can also be an important reservoir of the agent to humans [45].

Q fever has long been considered as an occupational zoonosis of major socio-economic importance worldwide associated with exposure to livestock by farmers, veterinarians, slaughterers, and animal researchers [56]. Its outbreaks have been occasionally observed in many countries throughout the world [14, 16, 27, 46, 55]. Despite the fact that the disease is widely distributed, the disease is regarded as neglected, under diagnosed and underreported because of its diverse symptoms, self-limiting course and lack of diagnostic tools [3, 7].

In the African Context Q fever was first reported in 1947, but since then the quantity and quality of epidemiological research on this pathogen has been limited [30]. Ethiopia was ranked highest in Africa in the health burden of zoonotic diseases [19]. The first evidence of C. burnetii was reported in ticks collected from cattle in Ethiopia [41]. As well as seroprevalence of C. burnetii was found to be 6.5% by complement fixation test in workers at Addis Ababa abattoir in goat and sheep slaughterhouses and its peri-urban zone as found by [1]. To date, the only study in Ethiopian concerning cattle was conducted in southeast of the country using enzyme-linked immunosorbent assay (ELISA) by [20]. This reported a high seroprevalence of C. burnetii, (31.6% in cattle, 90.0% in camels and 54.2% in goats). A 6.4% prevalence of C. burnetii in Ethiopia was also report from different Ixodid ticks species by quantitative real time polymerase chain reaction targeting two different genes followed by multi-spacer sequence typing (MST) by [28].

In recent years, reports of abortion and infertility in domestic ruminants from different corners of Ethiopia are becoming a common concern [2, 21, 33]. The Jimma zone in the Oromia Region of Ethiopia is one of such areas in from 2013 to 2015 it faced the worst outbreak of abortion, whereby more than 11,487 cases were recorded in domestic ruminants (cattle, goats and sheep) (Jimma zone livestock health and production agency, 2015). The Oromia Region of Ethiopia is the region with the highest population of cattle in the country and the Jimma zone of Oromia Region is the main cattle producing zone in Oromia and the second in Ethiopia with an estimated cattle population of 2,090,000 [17]. The unusually high losses of pregnancies and the resultant infertility in cattle within the Oromia region represent a tremendous economic loss to the nation and it is also a significant blow to the livelihoods of livestock producers in Ethiopia. The initial suspicions of Brucella involvement as a cause of the abortion cases in Oromia was ruled out by [12]. Coxiella was suspected to be one of the potential causes of such abortion episodes, as it can affect all three ruminant species. Nevertheless, to date there was no empirical evaluation of the level of seropositivity of cattle to C. burnetii in this important cattle producing zone of Ethiopia.

In this study, we aimed to identify the public health risk of C. burnetii to dairy farmers and communities in Jimma town in the Oromia region of Ethiopia with the objective of estimating the seroprevalence of C. burnetii and associated risk factors in cattle at Jimma dairy farms and its main slaughterhouse.

Results

A total of 227 and 195 samples were collected from Jimma’s dairy farms and slaughterhouse respectively. The overall seroprevalence was 8.77% (95%CI: 6.07–11.47); C. burnetii seropositivity was significantly lower in dairy farms (6.17%; 95% CI: 3.41–10.13) compared to slaughterhouse (11.79%; 95%CI: 7.27–16.32%) (p-value≤0.042).

Dairy farm-level C. burnetii seropositivity and its risk factors

Out of 227 animals included in the dairy farm analysis, the majority [n = 129 (56.83%)] originated from intensive management system and the vast majority were female [n = 223 (98.24%)]. Concerning their breed, the majority [n = 220 (96.92%)] were crossbred (Table 1) and in terms of age the minimum age sampled was 6 months and the maximum was 10 years. There was also higher seropositivity to C. burnetii in male cattle compared to female and higher seroprevalence in adult cattle compared to young. Prevalence of C. burnetii is found to be higher in the semi-intensive management system (8.16%; 95%CI: 3.59, 15.45) than in the intensive management system (4.65%; 95%CI: 1.73, 9.85) of dairy farms (Table 1).

Table 1 Univariable logistic regression analysis (adjusted for herd effect) to select forward factor for final model contributing to C. burnetii distribution in dairy cattle and slaughter cattle of Jimma, Ethiopia (n = 227; 195 respectively)

The final animal-level multivariable logistic regression mixed effect model showed that C. burnetii seropositivity is significantly positively associated with age (OR: 1.51(95%CI: 1.30, 1.75): p-value≤0.000) (Table 2). Our results also show that cattle managed in semi-intensive system were 8.08 more likely to be C. burnetii seropositive compared to intensively managed dairy cattle [OR = 8.08 (95%CI: 1.03, 63.68); P = 0.047]. Dairy cattle that have access to nuisance animals like dogs, cats, mice and other were 5.65 more likely to be C. burnetii seropositive compared to dairy cattle with no access to nuisance animals (Table 2). On the other hand, dairy cattle that have no tick infestation are 93% less likely to be seropositive for C. burnetii [OR = 0.07 (95%CI: 0.01, 0.74); P = 0.027] (Table 2).

Table 2 Results of final best fitting multivariable mixed effect generalized linear model for the probability of C. burnetii seropositivity in dairy cattle (n = 227) in Jimma, Ethiopia

Out of twenty-five dairy farms sampled, seven of them had at least one infected animal resulting in a herd-level C. burnetii seropositivity of 28% (95%CI: 12.07–49.39). Dairy farms which had at least one contact with other herds were 4.63 time more likely C. burnetii seropositive than herd which had no contact [OR = 4.63 (95%CI: 0.79, 26.94)] but that difference was marginally significant (Table 3).

Table 3 Multivariable Binomial Generalized linear models of factors at farm level (n = 25 farms) for C. Burnetii sero-distribution in dairy cattle of Jimma, Ethiopia

Slaughterhouse animals’ C. burnetii seroprevalence and their risk factors

The overall seroprevalence of C. burnetii antibodies from cattle sampled at slaughterhouse was found to be 11.79% (95%CI: 7.63, 17.17). Out of 195 animals included in the slaughterhouse analysis, all were from extensive management system, males and local breeds. All C. burnetii seropositive cattle were adults. Prevalence of C. burnetii antibody was found to be higher in tick infested cattle (12.14%) than the non-tick infested cattle (9.09%). Higher prevalence was recorded in medium body conditioned (16.22%) cattle compared to good body conditioned cattle (9.09%) (Table 1).

In the multivariable model of animals sampled at slaughterhouse age of cattle was the only factor found to be associated with C. burnetii seropositivity [OR = 2.27 (95%CI: 1.93, 2.68); p = 0.000], which means as age of cattle increase by 1 year, their chance of being C. burnetii seropositive increases by 2.27.

Discussion

This research is the first to investigate the seroprevalence of and risk-factors for C. burnetii exposure in cattle in Jimma Town the most important city in the second highest cattle production zone of Ethiopia. The i-ELISA test used was claimed to have 100% sensitivity (Se) and specificity (SP) as described by the manufacturer using serum from confirmed infected animals but other authors cited the test sensitivity and specificity for serum as 100 and 95%, respectively, compared to PCR [18]. Overall our results demonstrate that C. burnetii infection is a significant public health problem in the area in that 8.77% (95%CI: 6.07–11.47) of tested animals were found with evidence of C. burnetii antibodies. Our results suggest that cattle in Jimma town have a high level of exposure to C. burnetii infection which could partly explain the observed reproductive disorders and abortions occurring in Jimma zone. Our findings are in agreement with 7.9% report in Algeria (sample size 311, cross sectional and tested with ELISA) [8], but higher than similar studies undertaken in Bura, Tana River County, Kenya which reported 5% (Sample size 96, cross sectional study design and ELISA test) [35], and 4% in Chad (sample size 195, cross sectional with i-ELISA) [49]. However, the overall seroprevalence reported in our study is lower compared to the previous studies in the Southeast Ethiopia (i.e. a seroprevalence of 31.6% using cELISA) by [20], and other countries in Africa ranging between 13 and 32% [25, 26, 36, 38, 39, 50]. The possible reasons for these variations might be the difference in sample sizes, sampling methods and diagnostic tests used, geographical locations and management systems being practiced. Our results indicate that C. burnetii seroprevalence in cattle in Jimma town is significantly higher in cattle sent to slaughterhouse compared to dairy cattle in dairy farms (11.79% vs 6.17%) suggesting that management systems may play an important role at modulating exposure risk [6]. This might partly be explained by the fact that all cattle sampled at the slaughterhouse were local breeds kept under extensive management systems from a variety of different districts of Jimma zone. This finding is in line with the study conducted in Nigeria which reported a prevalence of 11% in cattle at slaughterhouse and a prevalence of 17.1 and 1.3% in local breed and cross breed respectively [54]. Extensive management systems allow for an increase in exposure opportunities to C. burnetii through aerosol transmission between animals at grazing and watering areas. The extensive management system also exposes cattle to wildlife which could play a relevant role for disease species cross-transmission [47].

Similarly, for dairy cattle our results indicate a significantly increased probability of seropositivity in crossbred dairy cattle kept under semi-intensive dairy production compared to intensive management system of dairy production. Cross breed dairy cattle are expensive and mostly kept under either intensive or semi-intensive management systems so that disease and tick are better controlled. Further, C. burnetii can survive in dry dusty environmental conditions for months and cattle managed in a semi-intensive system can be at greater risk of exposure to contaminated aerosols from known transmission vehicles from infected animals such as urine, feces or birthing products in the field compared to cattle managed in intensive systems [9]. This is in agreement with other studies showing that dairy cows which were partially grazing in the field had higher seropositivity to C. burnetii antibodies [6]. In addition, our study demonstrated that dairy cattle with access to nuisance animals (such as dogs, cats, mice) were more likely to be seropositive to C. burnetii antibodies compared to dairy cattle with no access to nuisance animals. This finding is supported by evidence suggesting the ability for a range of companion animals and pests to be infected with C. burnetii [4, 22, 32, 42, 43].

In our study we found a significant increase in the probability of C. burnetii exposure with increasing age in both dairy cattle and cattle sampled at slaughterhouse. This finding is in agreement with previous studies in Ethiopia and Cameroon [20, 30] and a more recent study by [24] describing the age distribution of C. burnetii antibodies in camels, cattle, goats and sheep. One possible explanation is that the older the animal the greater is the potential exposure to the pathogen infections and keep circulating antibody [5, 31]. On the other hand, the result indicates that dairy cattle with evidence of tick infestation had significantly higher increase in the probability of C. burnetii seropositivity (p-value≤0.027). This result is also in line with evidence from around the world pointing for the isolation of C. burnetii from ticks [23, 26, 28] indicating a potential role of tick infection in the dissemination of Q fever in the herd.

Our results indicate that dairy farm-level seroprevalence was marginally higher in farms with contact with other herds. Previous research reported that partial housing of the herds, contact with other herds and extensive management systems increased the likelihood of seropositivityto C. burnetii [6, 24, 48, 53, 57]. These are all modifiable farm-level bio-exclusion factors which can be acted upon by farmers to reduce the changes of C. burnetii transmission into the herd.

The findings of this study carry significant public health implications for the need to control Q fever in the community. Our results indicate that there is a significant risk of Q fever particularly in slaughterhouse workers, dairy farmers and other animal workers and the consumers of dairy products in Jimma town. Our results suggest that the burden of Q fever in these occupational groups identified is likely to be high and a collective effort is needed to investigate its impact on human health as well as to improve health promotion and education to these target community groups. Q fever awareness campaigns and on-farm Q fever biosecurity management plans need to be implemented in Jimma slaughterhouse workers and dairy cattle farmers with the aim of reducing their risk of exposure to C. burnetii. Furthermore, the level of seroprevalence demonstrated in dairy farms necessitates more attention because these animals are the milk source for children. The veterinarian and public health sector need to work together in a One health approach to investigate the shared burden of Q fever in the province of Oromia.

The findings of this study should be interpreted in light of its limitations. First, the cross-sectional nature of our investigation coupled with the use of serological tests for ascertainment of C. burnetii exposure means that we were unable to conclude on the true infection status of animals/herds. Second, from the slaughterhouse survey we were unable to include female cattle which are usually managed under extensive management systems and thereby provide a more complete epidemiological picture of the level of C. burnetii infection in rural population.

Conclusion

The present study indicates that C. burnetii exposure is significantly high in cattle in an area in Ethiopia with one of the highest cattle populations in the country. Our findings demonstrate important modifiable farm-level risk factors which can be used to design farm-level Q fever biosecurity management plans and Q fever health promotion campaigns to reduce the public health of risk of C. burnetii exposure. Further studies should be designed to investigate the level of C. burnetii exposure in dairy farmers, slaughterhouse workers and consumers of dairy production in the region.

Methods

Study area and period

This study was conducted in Jimma Town from October 2016 to October 2017. The town is located in the Jimma zone of Oromia Regional State, South Western Ethiopia (Fig. 1). Jimma town is situated at a distance of 356Km, South West of Addis Ababa, the capital city of Ethiopia, between 7°41“N latitude and 36°50”E longitudes and has an altitude of 1704 m above sea level. The climate of the area is a tropical humid climate characterized by heavy rainfall which ranges from 1200 to 2000 mm per annum. With the mean annual minimum and maximum temperature ranging from 6 °C and 31 °C respectively, the overall average temperature is approximately 18.5 °C. Jimma zone is one of the largest in livestock populations in Ethiopia with cattle population estimated 2,212,962 heads [10]. Dairy cattle are more under production in Jimma town and the surroundings small towns but more than 95% of the cattle populations are under extensive management which are used for mixed dairy and meat production as well as cash income generation for the rural communities.

Fig. 1

Study area and location of study farms (Map created by ArcGIS® software Esri version 10.8 (https://desktop.arcgis.com/en/arcmap/))

Target and study population

The target population was apparently healthy crossbred dairy cattle kept under intensive and semi-intensive management systems and local breed cattle which are kept under extensive management system. These involved smallholder dairy farms and Jimma Dairy Development Enterprise (JDDE) and the local breed of male cattle presented to slaughterhouse aged between 3 and less than 10 years.

Sample size determination

The sample size to arrive at the study population was determined using the formula described ((Z² x P(1- P))/e² where Z = 1.96 from normal distribution table, P = expected prevalence, e = desired precision level) by [13]. The conservative estimate of 50% prevalence, 95% level of confidence and 5% absolute precision was used. Accordingly, the estimated sample size of 384 animals was obtained. The calculated sample size was oversampled by 10% to account for possible problems with non-response or missing data [37]. This allowance was added summing up to the total of 422 samples. These samples were approximately halved to be distributed to dairy farms and slaughterhouse for blood sample collection. The proportion of required number of samples from each dairy farm was obtained by multiplying 28.3% expected prevalence of C. burnetii in cattle reported from Kenya [26] to the total number of cattle in each dairy farm; on average a total of 9 animals were sampled from each dairy farm.

Study design and sampling strategy

Two cross sectional studies were designed to achieve the objectives of this study. First, a slaughterhouse survey was designed in the following way: in each day of visit to the slaughterhouse for a period of 2 weeks, a representative percentage of 25% of animals were picked by simple random sampling technique from the lairage during ante mortem inspection. The sampling frame was constructed by listing the total number of animals in the lairage of each visiting day. The total number of slaughtered animals in Jimma slaughterhouse ranged from 55 to 85 per day. On average, 14 samples were sampled per day to attain the total samples required (ie. 195 samples) and after sampling, animal level data like age, sex, tick infestation, breed, body condition score, production system were recorded.

Second, a farm-level survey was designed to measure Q fever exposure in the following way: a list of all 61 dairy farms and their contact details and location (ie. Kebele) was obtained from Jimma town livestock and fisheries resources development office. Thus a total of 25 dairy farms were selected by simple random sampling technique out of the 61 farms on the list to satisfy the total sample required from dairy farms. Each farm was visited once for about 1 month sampling period. All targeted farms are business oriented dairy farms with crossbred and/or pure exotic breeds of dairy cattle (Holstein- Friesian). Based on [34], herd size was categorized as small if the total number of animals in the herd was 3–10 animals, and large if the animal number in the herd were 11 or above. A farm owner questionnaire was used to collect risk factor data for Q fever infection, including individual-level data and farm-level data. For individual-level data animals’ age in years was recorded by means of dentition (as described by [29]) and also asking farm owners. Additional individual-level independent variables included sex, body condition score (BCS; categorized as poor, good and very good as described by [44]), breed, tick infestation status of animals and animal parity, and abortion status. For farm-level data these included multi species mix, multi age mix, history of contact with other herds, herd size (in a continuous numeric scale), production system (classified as intensive, semi-intensive and extensive), presence of nuisance animals in the farm (eg. presence of dogs, cats, rodents and others), were included in the questionnaire/check list (Additional file 1: Appendix 1).

Specimen collection procedure

About 10 ml of blood sample was collected from the jugular vein of each selected cattle using plain vacutainer tubes and multipurpose disposable blood collection needle 21Gx1 1/2″ plus needle holder (Zhejiang Kanshi) Medical Devices Co. Ltd. (HENSO). Before and after sample collection, 70% ethanol alcohol was applied as disinfectant. Each specimen was labeled with unique identification number. The tubes were transported to Jimma University College of Agriculture and Veterinary Medicine laboratory in an icebox and the tubes were put in an oblique position of 45°, for overnight at room temperature, to allow clotting of blood, the next morning sera was gently pipetted into cryovials and stored in deep freezer at − 20 °C, until diagnosis was made in the laboratory of National Veterinary Institute (NVI) at Debre-Zeit, Ethiopia.

Laboratory analysis and interpretation

All serum samples were tested using Indirect Enzyme-Linked Immunosorbent Assay (i-ELISA) from ID Screen®Q fever Indirect Multi- Species kits (ID.vet, 310; rue Louis Pasteur–Grabels–France) for the detection of antibodies against C. burnetii. All reagents were prepared and results were interpreted according to the manufacturer’s instructions. Briefly, the optical densities (OD) were read at 450 nm in a micro-plate photometer (Multi Skan Ex, Thermo Electron Corporation, Finland). Negative control (NC), and positive control (PC) were run as duplicates in the micro–plate wells A, B and C, D respectively whereas sera were run as a single spot in the remaining micro plate wells. Interpretation of the result for each sample was obtained as the percentage of the ratio between the sample Optical Density (OD) and positive control OD, according to the $\frac{S}{P}\%$ formula as given below.

$$ \frac{S}{P}\%\frac{OD\; sample- OD\; negative\kern0.17em control}{{}^{-} OD\; positive\kern0.17em control- OD\; negative\kern0.17em control}x100. $$

The negative and positive samples were determined based on the laboratory test thresholds–values for its status (Table 4). The coloration quantity depends on the presence of antibodies in the specimen; positive sample will remain colored after addition of stop solution, while the light yellow negative sample will be colorless or white.

Table 4 Serum and plasma samples thresholds–values and status for the interpretation of ELISA test

Data management and statistical analysis

All data collected during the sero-surveys were entered into MS Office Excel 2010. Data were analyzed separately for cattle sampled in dairy farms and cattle sampled at the slaughterhouse. The overall prevalence was calculated as a total number of positive samples for C. burnetii divided by the total number of samples tested multiplied by 100. For each prevalence, binomial ‘exact’ 95% confidence interval (CI) was calculated using Epitools [51]. To statistically test the difference between the overall prevalence in dairy farms and slaughterhouse, a test for two sample proportions was calculated using the proportion test calculator in the statistical software STATA version 13 [52]

Univariable mixed effect logistic regression analysis was used to select individual explanatory variable that may predict individual C. burnetii seropositivity. Variables with a p-value < 0.25 at the univariable screening were taken forward to a multivariable mixed effect generalized linear model (farm as random effect) with Bernoulli family with a logit link. A separate multivariable binomial generalized linear model was used to model herd level prevalence data. Slaughterhouse data was analyzed using logit generalized linear model. Furthermore, multicollinearity was also assessed for any correlation between the explanatory variables with Spearman’s rank correlation and between management system and contact with other herds shows there is a correlation (Spearman’s rho = − 0.6001; P-value≤0.0015). Interaction terms between explanatory variables were entered into the model to investigate the presence of effect modification. Statistical significance in the multivariable model was set at a P–value ≤0.05. All statistical analyses were performed in Stata statistical software version 13 [52].