Table 4 Types, dilutions, and other features of antibodies used to detect different proteins. The letter in the primary antibody dilution indicates the secondary dilution used
Antibody | Dilution | Brand | Catalog N° |
|---|---|---|---|
Sequestosome 1 (P62/SQSTM1) | 1:1000a | Abcam | 91,526 |
Microtubule-associated protein 1A/1B-light chain 3 (LC3A/B) | 1:1000a | Cell Signaling | 12,741 |
Phospho-Extracellular signal-regulated kinases 1/2 (pERK1/2) | 1:2000c | Cell Signaling | 9106 |
Extracellular signal-regulated kinases 1/2 (ERK1/2) | 1:2000c | Cell Signaling | 9107 |
Phospho-Eukaryotic translation initiation factor 4E-binding protein 1 (p4E-BP1) | 1:1000a | Cell Signaling | 9459 |
Eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) | 1:1000a | Cell Signaling | 9452 |
Ubiquitin | 1:2000b | Cell Signaling | 3933 |
Histone 2B (H2B) | 1:2000b | Abcam | 17,901 |
Anti-rabbit IgG | 1:2000a | Cell Signaling | 7074 |
Anti-rabbit IgG | 1:4000b | Cell Signaling | 7074 |
Anti-mouse IgG | 1:4000c | Cell Signaling | 7076 |