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Table 4 Types, dilutions, and other features of antibodies used to detect different proteins. The letter in the primary antibody dilution indicates the secondary dilution used

From: Effects of crowding on the three main proteolytic mechanisms of skeletal muscle in rainbow trout (Oncorhynchus mykiss)

Antibody Dilution Brand Catalog N°
Sequestosome 1 (P62/SQSTM1) 1:1000a Abcam 91,526
Microtubule-associated protein 1A/1B-light chain 3 (LC3A/B) 1:1000a Cell Signaling 12,741
Phospho-Extracellular signal-regulated kinases 1/2 (pERK1/2) 1:2000c Cell Signaling 9106
Extracellular signal-regulated kinases 1/2 (ERK1/2) 1:2000c Cell Signaling 9107
Phospho-Eukaryotic translation initiation factor 4E-binding protein 1 (p4E-BP1) 1:1000a Cell Signaling 9459
Eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) 1:1000a Cell Signaling 9452
Ubiquitin 1:2000b Cell Signaling 3933
Histone 2B (H2B) 1:2000b Abcam 17,901
Anti-rabbit IgG 1:2000a Cell Signaling 7074
Anti-rabbit IgG 1:4000b Cell Signaling 7074
Anti-mouse IgG 1:4000c Cell Signaling 7076