Fig. 2From: Effects of crowding on the three main proteolytic mechanisms of skeletal muscle in rainbow trout (Oncorhynchus mykiss)Effect of high density-induced stress on the IGF-1 system. a Representative Western blots of key genes of IGF-1 system, ERK and p4E-BP1, in phosphorylated and total forms, with H2B as the loading control. b Fold change of pERK/ERK ratio (c) Fold change of p4E-BP1/4E-BP1 ratio. Results are shown as the means ± SEM (n = 5 per group). LD, low stocking density; HD, high stocking density. Significant differences are indicated with a line (* = P < 0.05)Back to article page