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Table 2 Primers used for amplification of each gene in this study

From: Molecular detection and genetic characterization of Mycoplasma gallisepticum, Mycoplama synoviae, and infectious bronchitis virus in poultry in Myanmar

Target genePrimer namePrimer sequences (5′ – 3′)Size in nucleotides (bp)References
For detection of each pathogen
 pMGA1.2
  1stpMGAFoGTG AAG AAA AAA AAC ATA TTA AAG TTT1,900Mardassi et al., 2005 [15]
pMGARoCTA AGA TGG ATT TGA AAC ATT AGT
  2ndpMGAF1iCTA GTT AAT ACT AGT GAT CAA GTG AAA CTA500Mardassi et al., 2005 [15]
pMGAR1iTTG AAC ATT GTT CTT TGG AAC CAT CAT
 MS2/12
  1stMS1.2FoAAA CTA CAA AAC TTT GTA ATG GCT1,200Mardassi et al., 2005 [15]
MS1.2RoTTA CAA GTA CGG TGT TTA ATC AAT
  2ndMS1.2F1iATT ACC AAG CAG ATG GTT ACG ACG T450Mardassi et al., 2005 [15]
MS1.2R2iAGT TAT AGT AAC TCC GTT TGT TCC A
S1IBV-S1AGG AAT GGT AAG TTR CTR GTW AGA G620–640Mase et al., 2004 [16]
IBV-S2GCG CAG TAC CRT TRA YAA AAT AAG C
For sequence analysis
gapAgapA 3FTTC TAG CGC TTT AGC CCT AAA CCC332Ferguson et al., 2005 [17]
gapA 4RCTT GTG GAA CAG CAA CGT ATT CGC
vlhAvlhA fTAC TAT TAG CAG CTA GTG C350 / 400Dijkman et al., 2016 [18]
vlhA RAGT AAC CGA TCC GCT TAA T