Molecular detection and genetic characterization of Mycoplasma gallisepticum, Mycoplama synoviae, and infectious bronchitis virus in poultry in Myanmar

Table 2 Primers used for amplification of each gene in this study

Target gene	Primer name	Primer sequences (5′ – 3′)	Size in nucleotides (bp)	References
For detection of each pathogen
pMGA1.2
1st	pMGAFo	GTG AAG AAA AAA AAC ATA TTA AAG TTT	1,900	Mardassi et al., 2005 [15]
1st	pMGARo	CTA AGA TGG ATT TGA AAC ATT AGT	1,900	Mardassi et al., 2005 [15]
2nd	pMGAF1i	CTA GTT AAT ACT AGT GAT CAA GTG AAA CTA	500	Mardassi et al., 2005 [15]
2nd	pMGAR1i	TTG AAC ATT GTT CTT TGG AAC CAT CAT	500	Mardassi et al., 2005 [15]
MS2/12
1st	MS1.2Fo	AAA CTA CAA AAC TTT GTA ATG GCT	1,200	Mardassi et al., 2005 [15]
1st	MS1.2Ro	TTA CAA GTA CGG TGT TTA ATC AAT	1,200	Mardassi et al., 2005 [15]
2nd	MS1.2F1i	ATT ACC AAG CAG ATG GTT ACG ACG T	450	Mardassi et al., 2005 [15]
2nd	MS1.2R2i	AGT TAT AGT AAC TCC GTT TGT TCC A	450	Mardassi et al., 2005 [15]
S1	IBV-S1	AGG AAT GGT AAG TTR CTR GTW AGA G	620–640	Mase et al., 2004 [16]
S1	IBV-S2	GCG CAG TAC CRT TRA YAA AAT AAG C	620–640	Mase et al., 2004 [16]
For sequence analysis
gapA	gapA 3F	TTC TAG CGC TTT AGC CCT AAA CCC	332	Ferguson et al., 2005 [17]
gapA	gapA 4R	CTT GTG GAA CAG CAA CGT ATT CGC	332	Ferguson et al., 2005 [17]
vlhA	vlhA f	TAC TAT TAG CAG CTA GTG C	350 / 400	Dijkman et al., 2016 [18]
vlhA	vlhA R	AGT AAC CGA TCC GCT TAA T	350 / 400	Dijkman et al., 2016 [18]

ISSN: 1746-6148