Fig. 4

Protein expressions of IRS-1 and GLUT4 were down regulated in differentiated 3T3-L1 adipocytes after blocking of FGF1 function. a FGF-1 concentrations in cAT-MSC CM and DMEM were measured by ELISA assay. b Protein expression levels of GLUT4 and IRS-1 were analysed by western blot. c, d Relative protein levels of IRS-1 and GLUT4 were lower in the insulin resistance models treated with both cAT-MSC CM and anti-FGF1 antibody than the models treated with cAT-MSC CM alone. Naïve: differentiated 3T3-L1 cells, (−): insulin resistance models of 3T3-L1 cells, cAT-MSC CM: cAT-MSC CM-treated insulin resistance models of 3T3-L1 cells, cAT-MSC CM + Ab: cAT-MSC CM- and anti-FGF1 antibody-treated insulin resistance models. Results are presented as mean ± SD obtained from three independent experiments *P < 0.05, **P < 0.01