Table 1 Primer designed to detect goatpox and sheeppox virus by duplex PCR
From: Development of duplex PCR for differential detection of goatpox and sheeppox viruses
Primer name | Length | Sequence(5′-3′) | Notes |
|---|---|---|---|
E10R-f | 41 | CCGCTCGAGGCCACCATGAATCCTAAACACTGGGGAAGAGC | The universal primers for GTPV and SPPV RPO40 gene, the predicted length of PCR is 285 bp. |
E10R-r | 36 | CGCGGATCCCGAAGCGGTAATACCTTATTTAAATTG | |
RPO132-f | 39 | CCGCTCGAGGCCACCATGAATAGGTTCAAGGAAAAGCAT | The special PCR primers for SPPV RPO132 gene, the predicted length of Lamp is 746 bp. |
RPO132-r | 30 | CGCGGATCCCGCATTATTTTTTCATACGAT |