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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Development of duplex PCR for differential detection of goatpox and sheeppox viruses

Fig. 4

Optimization of reaction cycles for duplex PCR reaction in the detection of GTPV or SPPV using mix primers. Agarose gel electrophoresis showing the effect of cycles on duplex PCR. a Duplex PCR amplicated products using 100 ng SPPV gDNA as template. b Duplex PCR amplicated products using 100 ng GTPV gDNA as template. Lane 1–5: Reaction annealing temperature is 50 °C for 20 cycles, 25 cycles, 30 cycles, 35 cycles and 40 cycles; Lane C: No template control and Lane M: 2000 bp DNA Ladder Marker (TaKaRa, Dalian)

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