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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Development of duplex PCR for differential detection of goatpox and sheeppox viruses

Fig. 3

Optimization of primers rate for duplex PCR reaction. Lane 1–5 and 1′-5′: E10R primers is 0.5 μM, RPO132 primers is 0.2 μM, 0.4 μM, 0.6 μM, 0.8 μM and 1 μM, respectively; Lane 6–10 and 6′-10′: RPO132 primers is 0.5 μM, E10R primers is 0.2 μM, 0.4 μM, 0.6 μM, 0.8 μM and 1 μM, respectively. Lane1–5 and 6–10: 100 ng SPPV genome as templates; Lane1’-5’and 6′-10′: 100 ng GTPV genome as templates, respectively. Lane C and C′: No template control. Lane M:2000 bp DNA Ladder Marker (TaKaRa, Dalian). The strip in red grid showed the result is better using 1 μM RPO132 primers and 0.2 μM E10R primers in the reaction

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