Salmonella enterica serovar Enteritidis (S. Enteritidis) is a highly adaptive pathogen in both humans and animals. As a Salmonella Type III secretion system (T3SS) effector, Salmonella protein tyrosine phosphatase (SptP) is critical for virulence in this genus. To investigate the feasibility of using C50336ΔsptP as a live attenuated oral vaccine in mice, we generated the sptP gene deletion mutant C50336ΔsptP in S. Enteritidis strain C50336 by λ-Red mediated recombination and evaluated the protective ability of the S. Enteritidis sptP mutant strain C50336ΔsptP against mice salmonellosis.
Results
We found that C50336ΔsptP was a highly immunogenic, effective, and safe vaccine in mice. Compared to wild-type C50336, C50336ΔsptP showed reduced virulence as confirmed by the 50% lethal dose (LD50) in orally infected mice. C50336ΔsptP also showed decreased bacterial colonization both in vivo and in vitro. Immunization with C50336ΔsptP had no significant effect on body weight and did not result in obvious clinical symptoms relative to control animals treated with phosphate-buffered saline (PBS), but induced humoral and cellular immune responses at 12 and 26 days post inoculation. Immunization with 1 × 108 colony-forming units (CFU) C50336ΔsptP per mouse provided 100% protection against subsequent challenge with the wild-type C50336 strain, and immunized mice showed mild and temporary clinical symptoms as compared to those of control group.
Conclusions
These results demonstrate that C50336ΔsptP can be a live attenuated oral vaccine for salmonellosis.
Salmonella spp. is a Gram-negative, facultative anaerobe and intracellular pathogen in both humans and animals. Infection by Salmonella is a major public health problem [1], causing an estimated 93.8 million illnesses and 155,000 deaths each year worldwide [2] and more than 1 million illnesses and 350 deaths each year in the U.S. [3]. In the past 20 years, Salmonella enterica serovar Enteritidis (S. Enteritidis) has been one of the most common serotypes in salmonellosis in humans despite the implementation of control and prevention measures [4]. Humans can be infected with Salmonella via consumption of contaminated pork, beef, poultry, and eggs or contact with fecal matter in places with poor sanitation. Salmonellosis in humans is characterized by abdominal pain, diarrhea, nausea, vomiting, fever, and headache [5].
Salmonellosis treatment and protection strategies include antimicrobial therapy and vaccination, but emergence of multidrug-resistant strains is becoming a serious global problem [6, 7]. Vaccines based on inactivated bacteria can potentially prevent salmonellosis [8]; however, attenuated live vaccines generated by deletion of various identified Salmonella virulence genes have higher immunogenicity and greater efficacy than killed bacteria [9–14].
Salmonella protein tyrosine phosphatase (SptP) is a Salmonella T3SS effector protein encoded in Salmonella pathogenicity islands (SPI)-1. The SptP protein has an N-terminal domain that acts as a GTPase-activating protein for Cdc42 and Rac1, mediating alterations in the actin cytoskeleton of host cells [15], as well as a C-terminal domain that inhibits mitogen-activated protein kinase and extracellular signal-regulated kinase signaling [16]. SptP also suppresses interleukin-8 (IL-8) production and consequently the inflammatory response in hosts, thereby promoting Salmonella invasion and intracellular replication [17, 18]. In a mouse infection model, SptP suppressed the degranulation of mast cells and blocked neutrophil recruitment [19].
In this study, we generated the sptP gene deletion mutant C50336ΔsptP in S. Enteritidis strain C50336 by λ-Red mediated recombination [20]. The growth characteristics of C50336ΔsptP were similar to those of wild-type C50336. We therefore investigated the feasibility of using C50336ΔsptP as a live attenuated oral vaccine in mice by evaluating virulence, changes in body weight and clinical symptoms, bacterial persistence, immune responses, and protective efficacy.
Methods
Bacterial strains and cells lines
The wild-type S. Enteritidis strain C50336 was obtained from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). The sptP deletion mutant strain C50336ΔsptP was constructed by λ-Red-mediated recombination as previously described [20]. Briefly, the sequence of the chloramphenicol resistance cassette (CmR) was amplified from plasmid pKD3, including 39-bp homology extensions at the 5′ and 3′ ends of the sptP gene (primers: forward 5′-tgaatcagcaggaagtgctcaaaaacatactgcaggaatgtgtaggctggagctgcttc −3′; reverse 5′-cttactttcagatagttctaaaagtaagctatgtttttaatgggaattagccatggtcc −3′). PCR products were purified and transferred into C50336 cells containing plasmid pKD46 by electroporation. Recombinant C50336-CmR cells grown on Luria-Bertani (LB) agar plates were selected for both CmR and ampicillin resistance (AmpR). Allelic replacement of sptP with the Cm cassette was verified by PCR analysis (primers: forward 5′-atccgaactactttacgc-3′; reverse 5′-tgaatggtattctactgg-3′) and DNA sequencing. The cassette was then excised by introducing the Flp recombinase-expressing vector pCP20. Biochemical tests were performed using the API 20E identification kit (BioMérieux, Lyon, France) and VITEK 2 Gram-negative bacilli test (BioMérieux) according to the manufacturer’s protocol. Bacteria were cultured in LB broth followed by overnight incubation at 37 °C with shaking at 180 rpm [21]. XLT4 (Difco Laboratories, San Jose, CA, USA) and 1.5% LB agar were used for bacterial culture and counts of colony-forming units (CFU).
Human epithelial cells Caco-2 BBE and mouse macrophage RAW264.7 cells were cultured and maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS), 50 μg/ml streptomycin, and 50 U/ml penicillin.
Experimental animals
Female BALB/c mice (8 weeks old) used for vaccination experiments were obtained from the Comparative Medical Center of Yangzhou University (Yangzhou, China). The animal experiments were all approved by the Animal Care and Ethics Committee of Yangzhou University, Yangzhou, China (Approval ID: SYXK [Su] 2012–0029).
Assessment of bacterial virulence
The virulence of mutant C50336ΔsptP and wild-type strain C50336 was evaluated in BALB/c mice by oral inoculation of the mice with various doses of the bacterial strains. The morbidity and mortality of the mice were observed as previously described [22]. Briefly, water and food were withdrawn 4 h before oral gavage with 100 μl of 5% sodium bicarbonate to neutralize stomach acid; 1 h later, mice were administered 10-fold dilutions of C50336ΔsptP or C50336 [1 × 108–1 × 104 CFU in 100 μl of phosphate-buffered saline (PBS)] by oral gavage. Control mice received 100 μl of PBS via the same route. All deaths of mice were recorded over the 16-day experimental period. The LD50 was calculated using the Karber and Behrens method [23].
Bacterial colonization in cells
Human epithelial cells Caco-2 BBE and mouse macrophage RAW264.7 were grown in DMEM with 10% FBS. At 90–100% confluence, the monolayers were washed three times with PBS, and then the cells were colonized with an equal number of the indicated bacteria for 30 min (multiplicity of infection = 100). For bacterial adhesion, the cells were washed, and then incubated with PBS containing Triton X-100 (0.5%) at 37 °C for 10 min. For bacterial invasion, 30 min after bacterial colonization, the cells were incubated for an additional 30 min in DMEM with gentamicin (100 μg/ml), washed and incubated with PBS containing Triton X-100 (0.5%) at 37 °C for 10 min [24]. Serial 10-fold dilutions of cell lysates were plated on XLT4 agar and incubated at 37 °C for 12–16 h. The number of bacteria was counted and expressed as 102 CFU/ml.
Bacterial colonization and persistence in organs and tissues
Bacterial colonization and persistence in the internal organs of infected mice were evaluated. Mice were administered 1 × 108 or 1 × 107 CFU of C50336ΔsptP by oral gavage, and six mice per day were sacrificed at 1, 3, 5, 7, 14, 21, 28, and 44 days post-immunization (DPI). Liver, spleen, and mesenteric lymphadenitis (mLN) samples were aseptically collected, weighed, and homogenized in 1 ml of PBS; Serial 10-fold dilutions of tissue homogenates (100 μl each) were plated on XLT4 agar and incubated at 37 °C for 12–16 h. Bacteria were counted and the numbers are expressed as log10 CFU/g.
Changes in body weight and clinical symptoms after immunization
Mice were immunized with C50336ΔsptP at 1 × 108 or 1 × 107 CFU in 100 μl of PBS by oral gavage (two doses of immunization at 0 and 14 DPI). Control animals received 100 μl of PBS. Body weight was recorded at 1, 3, 5, 7, 14, 21, 28, and 44 DPI. The mice were monitored from 1 to 44 DPI for clinical symptoms, including feed intake, susceptibility, depression and diarrhea.
Serum IgG test
Specific IgG antibody and IgG subtype levels were measured by enzyme-linked immunosorbent assay (ELISA) using soluble antigens prepared from S. Enteritidis strain C50336 (SEAgP) as the coating antigen in 96-well plates (100 μl of SEAgP at 2 μg/ml in each well). Serum samples were collected from mice at 12 and 26 DPI and diluted 1:50 for use as the primary antibody. Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (or anti-mouse IgG1 and IgG2a for IgG subtype detection) was used as the secondary antibody (dilution 1:10,000). HRP activity was determined using 3,3′,5,5′-tetramethylbenzidine (Sigma-Aldrich, St. Louis, MO, USA). Absorbance was read at 450 nm using an automated microplate reader (Titertek Multiskan; Flow Laboratories, Lugano, Switzerland).
Lymphocyte stimulation test
The lymphocyte proliferation assay was performed using SEAgP as a stimulator. The spleen was isolated from the mice at 12 and 26 DPI and homogenized. Splenic lymphocytes were obtained by passing the homogenate through a filter with 40-μm pores (BD Biosciences, San Jose, CA, USA). Cell viability was determined based on Trypan Blue dye exclusion. Spleen mononuclear cell suspensions (1 × 107 cells/ml) were cultured in Roswell Park Memorial Institute 1640 medium containing 10% FBS, 50 μg/ml streptomycin, and 50 U/ml penicillin in 96-well tissue culture plates with 10 μg/ml SEAgP or 10 μg/ml concanavalin A (Con A, a lymphocyte mitogen) as a positive control at 37 °C in a humidified atmosphere of 5% CO2 for 72 h. Lymphocyte proliferation was measured with a BrdU kit (Roche, Basel, Switzerland). Blastogenic responses to SEAgP are expressed as a mean stimulation index (SI) calculated based on the optical density of stimulated cultures at 450 nm, as previously described [25, 26].
Immune protection assessment of C50336ΔsptP
The protective efficacy of the mutant C50336ΔsptP was evaluated in mice immunized orally with 1 × 108 CFU (group A) in 100 μl of PBS (two doses of immunization at 0 and 14 DPI). Control mice (groups B and C, respectively) received 100 μl of PBS. At 28 DPI, group A was challenged orally with 1 × 108 CFU of wild-type strain C50336 in 100 μl of PBS, whereas group B was challenged with 5 × 105 CFU of wild-type strain C50336 in the same way. Group C received 100 μl of PBS as a blank control. The number of surviving mice and extent of bacterial colonization in internal organs were determined at 16 days post challenge. The number of CFUs recovered from tissues was replica-verified by PCR analysis (Primers: Forward 5′-atccgaactactttacgc-3′; Reverse 5′-tgaatggtattctactgg-3′). Clinical symptoms, including anorexia, diarrhea, depression, and mortality, were recorded daily.
Statistical analysis
Data are expressed as the mean ± SEM. All statistical tests were two-sided. P < 0.05 was considered to be statistically significant. Differences between two samples were evaluated using Student’s t test. Statistical analyses were performed using SAS v.9.4 software (SAS Institute, Cary, NC, USA).
Results
Construction of a sptP deletion mutant C50336ΔsptP in S. Enteritidis
The sptP gene deletion mutant was established in S. Enteritidis strain C50336 by λ-Red-mediated recombination. Our PCR data showed that the sptP gene was deleted in the C50336ΔsptP mutant (Fig. 1a). The growth characteristics of the C50336ΔsptP mutant and wild-type C50336 cells were determined in LB liquid medium, with no significant difference observed between the mutant and wild type (Fig. 1b). Biochemical tests were performed using the API 20E identification kit (BioMérieux, Lyon, France) and VITEK 2 Gram-negative bacilli test (BioMérieux) according to the manufacturer’s protocol, also showed no difference between these strains (data not shown).
Fig. 1
Construction of sptP deletion mutant in S. Enteritidis strain C50336. a PCR verification of C50336ΔsptP and C50336-CmR. The wild type strain harbors the complete sptP gene, with a PCR product length of 2509 bp, whereas the PCR product from C50336ΔsptP has a length of 1061 bp, and the product from the C50336-CmR has a length of 1991 bp (right). b Growth curves of wild-type S. Enteritidis C50336 and C50336ΔsptP. Bacteria were grown in liquid LB medium at 37 °C for 12 h with agitation, and the OD600 values of triplicate cultures in LB medium were determined in 1-h intervals
C50336ΔsptP exhibits reduced virulence in a murine model
The virulence of S. Enteritidis wild-type C50336 and C50336ΔsptP was evaluated in BALB/c mice with oral gavage. As shown in Table 1, the LD50 of C50336 was 3.16 × 105 CFU, but no mice died in the group challenged by C50336ΔsptP, indicating that the virulence of the S. Enteritidis mutant C50336ΔsptP was attenuated.
Table 1
LD50 of C50336ΔsptP in BALB/c mice
Strain
Inoculation dose (CFU)
Number of dead mice/total number of mice
LD50
C50336
1.0 × 107
5/5
3.16 × 105
1.0 × 106
4/5
1.0 × 105
1/5
1.0 × 104
0/5
C50336△sptP
1.0 × 108
0/5
—
1.0 × 107
0/5
1.0 × 106
0/5
1.0 × 105
0/5
1.0 × 104
0/5
sptP deletion leads to reduced bacterial colonization in cells
The bacterial colonization in cells was determined in human epithelial Caco-2 BBE and mouse macrophage RAW264.7 cells. As shown in Fig. 2, the total number of associated bacteria was not different between C50336ΔsptP and C50336 in Caco-2 BBE and RAW264.7 cells. However, cells colonized with C50336ΔsptP showed a decreased intracellular bacterial load compared to cells colonized with C50336 (Fig. 2a & b). These results indicate that S. Enteritidis with sptP deletion has reduced bacterial colonization in vitro.
Fig. 2
Bacterial colonization in cells. Salmonella adhesion and invasion in the Human epithelial Caco-2 BBE cells (a), and mouse macrophage RAW264.7 cells (b). The number of Salmonella was determined and presented as 102 CFU/mL. Data are expressed as the mean ± SEM. **P ≤ 0.01
Colonization and persistence of Salmonella C50336ΔsptP in internal organs and tissues
The bacterial number was calculated in the liver, spleen and mLN of BALB/c mice following oral immunization with 1 × 108 CFU or 1 × 107 CFU C50336ΔsptP. As shown in Fig. 3, Salmonella colonization reached the highest level at 3 DPI (first immunization) in the liver, spleen, and mLN (Fig. 3b–d), and at 28 DPI (14 days post second immunization), two and one of the six mice were positive for Salmonella in the liver and spleen, respectively, in both the 1 × 108 and 1 × 107 CFU- immunized groups. In the 1 × 107 CFU immunized group, Salmonella was detected in the mLN of one mouse. At 44 DPI, we did not detect Salmonella in any tissues from immunized mice. All samples from the negative control group (PBS group) were negative for Salmonella.
Fig. 3
Body weight (a) and bacterial colonization in mouse organs post immunization. Salmonella colonization and persistence in the liver (b), spleen (c), and mLN (d) of mice following oral immunization with 1 × 107 CFU or 1 × 108 CFU of C50336ΔsptP. Control mice received 100 μl of PBS and were negative for Salmonella in the liver, spleen, and mLN. The number of Salmonella was determined and represented as Log10 CFU/g
Immunogenicity of C50336ΔsptP
The changes in body weight and clinical symptoms were monitored in mice following immunization with C50336ΔsptP. The body weights of the mice were shown in Fig. 3a. A decrease in body weight loss was noticed in the initial days of post immunization, but no statistically significant differences were observed among the three groups (immunized with 1 × 108 CFU C50336ΔsptP, immunized with 1 × 107 CFU C50336ΔsptP, and treated with PBS as a control). No significant differences were found in clinical symptoms among the three groups, although slight and temporary anorexia was observed in vaccinated mice. Clinical signs of disease such as lethargy and diarrhea were absent in all immunized mice and control mice. The procedures for immunization are shown in Fig. 4a.
Fig. 4
Immunogenicity of C50336ΔsptP. Procedure for immunization (a). Determination of serum IgG levels (b) and IgG subtype levels (c) at 12 and 26 days post immunization. Stimulation index (SI) of mice lymphocytes determined by splenic lymphocyte proliferation assay using soluble antigen SEAgP (d) or lymphocyte mitogen ConA as a positive control. The vaccinated group received 1 × 107 CFU or 1 × 108 CFU of C50336ΔsptP orally, and the control group received 100 μl of PBS. Data are expressed as the mean ± SEM. *P ≤ 0.05, **P ≤ 0.01
The humoral immune responses were evaluated in mice following oral immunization with C50336ΔsptP. Serum IgG levels were determined by indirect ELISA. As shown in Fig. 4b, mice immunized with 1 × 108 CFU and 1 × 107 CFU of C50336ΔsptP showed significantly higher levels of serum IgG at 12 and 26 DPI than the control group. Furthermore, the titers of the IgG1 subtype were significantly higher than those of IgG2a, demonstrating that S. Entertidis C50336ΔsptP tends to induce a Th2 immune response in mice (Fig. 4c).
The cellular immune responses in mice following oral immunization with C50336ΔsptP were evaluated by splenic lymphocyte proliferation assay. As shown in Fig. 4d, the SI values of immunized mice were significantly higher than control mice after stimulation with SEAgP or lymphocyte mitogen ConA at 12 and 26 DPI. This finding indicates that C50336ΔsptP also induces cellular immune responses in mice.
C50336ΔsptP protects mice against oral challenge with wild-type S. Enteritidis
Mice were orally vaccinated with 2 doses of 1 × 108 CFU of C50336ΔsptP, and then at 28 DPI, they were challenged with wild-type C50336. The percentage of surviving mice at 16 days post challenge is shown in Table 2. None of the immunized mice died in group A and group C, whereas ten of fifteen mice died in control group B. Slight and temporary anorexia as well as depression were observed following challenge in the immunized mice (group A) when compared the blank control group C. Severe clinical symptoms and mortality were observed following challenge in the non-immunized mice. The survival curve is shown in Fig. 5a.
Table 2
Protective effects of C50336ΔsptP in mice
Group
Vaccination
Number
Challenge
Survivors/total
Survival rate (%)
Strain
Dose (CFU)
Strain
Route
Dose (CFU)
A
C50336ΔsptP
1 × 108
10
C50336
oral
1 × 108
10/10
100
B
PBS
—
10
C50336
oral
5 × 105
5/15
33
C
PBS
—
10
PBS
oral
—
5/5
100
Fig. 5
Survival curve (a) and bacterial colonization in challenged mice. Bacterial colonization in the liver (b), spleen (c) and cecum (d) of challenged mice (Table 2, group A, immunized with C50336ΔsptP and then challenged with 1 × 108 CFU of C50336; group B, immunized with PBS and then challenged with 5 × 105 CFU of C50336). All samples from the blank control group were negative for Salmonella (Table 2, group C). The number of bacteria was determined and expressed as log10 CFU/g. Data are expressed as the mean ± SEM, **P ≤ 0.01
All surviving mice were sacrificed at 16 days post challenge, and tissues including the liver, spleen and cecum were sampled. As shown in Fig. 5, for the group vaccinated with 1 × 108 CFU of C50336ΔsptP, four of ten immunized mice carried a low level of Salmonella in the liver and spleen (Fig. 5b & c), and two of ten mice were positive for Salmonella in the cecum (Fig. 5d). The mice in group B carried a higher level of Salmonella in the liver, spleen, and cecum than the immunized mice from group A. All samples from the blank control group (group C) were negative for Salmonella.
Discussion
In current study, we constructed an sptP mutant in S. Enteritidis C50336, and evaluated the efficacy of C50336ΔsptP as a candidate live attenuated vaccine for Salmonella infection based on virulence, changes in body weight and clinical symptoms, bacterial colonization, serum IgG level, splenic lymphocyte proliferation and protective efficiency in mice. Our results showed that the body weight change of mice orally vaccinated with C50336ΔsptP (1 × 107 CFU or1 × 108 CFU) was similar to that of the PBS control. After immunization with C50336ΔsptP, only small amounts of Salmonella colonized and persisted in the liver and spleen of the mice at 28 days, but strong humoral and cellular immune responses were induced to protect the mice against secondary S. Enteritidis challenge.
Salmonella enterica subsp. enterica includes several important serovars including Typhimurium, Enteritidis Typhi, and Paratyphi, which are the leading sources of human salmonellosis. Vaccines have been developed for one of these serovars and will prevent disease caused by S. Typhimurium or S. Enteritidis [27]. Symptoms of human Salmonellosis include abdominal pain, diarrhea, nausea, vomiting, fever, and headache [5]. S. Enteritidis is also increasingly reported in cases of invasive and extra-intestinal infections, such as arthritis, septicemia, meningitis, endocarditis, and urinary tract infections [28–34]. Infection with Salmonella is usually caused by consumption of contaminated pork and beef, poultry and eggs. It is necessary to take effective measures to control and prevent Salmonella infection. Vaccination with live attenuated Salmonella may be a viable choice. Many live attenuated vaccines have been developed against Salmonella and have been confirmed to be generally more effective than vaccines prepared with dead bacteria [9–14].
The ideal vaccine should be avirulent, especially for live attenuated vaccines. Some S. Typhimurium and S. Enteritidis mutants with deletion of T3SS effector encoding genes or SPI-1 or SPI-2 display decreased virulence in mice, poultry, pigs, and humans [35–40]. In the current study, the LD50 of C50336ΔsptP in mice inoculated by the oral route demonstrated that the virulence of the mutant C50336ΔsptP was significantly decreased in comparison with that of the wild-type strain. The body weight of mice immunized with C50336ΔsptP was not significantly different from that of control mice and no or less clinical symptoms were observed following immunization. All of these findings show that C50336ΔsptP has almost no side effects in terms of growth performance in mice.
For live attenuated Salmonella vaccines, it is critical to stimulate the humoral and cellular immune responses in the immunized host [8, 41]. Systemic dissemination of Salmonella can lead to antigen presentation, resulting in high induction of specific humoral and cellular immune responses, and efficient protection against secondary infection [12, 42]. In this study, the Salmonella-specific serum IgG level in mice immunized with C50336ΔsptP at 12 and 26 DPI was significantly higher than the antibody level detected in the control group. In addition, the SI values of splenic lymphocyte proliferation in immunized mice were significantly higher than that in control mice. All of these findings indicate that strong humoral and cellular immune responses can be stimulated by C50336ΔsptP. In addition, the mice vaccinated with 1 × 108 CFU C50336ΔsptP showed higher levels of serum IgG and splenic lymphocyte proliferation capability than mice vaccinated with 1 × 107 CFU C50336ΔsptP.
S. Enteritidis deleted with one or several effector encoding genes provided efficacious protection against secondary S. Enteritidis challenge in mice [12, 13]. In our study, to evaluate the protective efficacy of C50336ΔsptP, we orally immunized mice with 1 × 108 CFU of C50336ΔsptP, and determined protection rates following oral challenge with 1 × 108 CFU of wild-type C50336. Our results show that mice immunized with 1 × 108 CFU of C50336ΔsptP developed extensive humoral and cellular immune responses and experienced 100% protection against subsequent S. Enteritidis challenge.
Pathogenesis of Salmonella is associated with a specialized ability to invade and persist within intestinal epithelial cells, where it can replicate and evade the host immune system [43]. T3SS-1 and T3SS-2 are encoded by SPI-1 and SPI-2 respectively, secreting various effector proteins that are essential for Salmonella colonization and intracellular persistence in the host [11, 35, 44]. SptP is a T3SS-1 effector protein that mediates the reversion of pathogen-induced changes in the actin cytoskeleton after Salmonella invasion [15]. In our study, S. Enteritidis C50336 with deletion of sptP led to decreased invasion in Caco-2 BBE and RAW264.7 cells. SptP also inhibits the MAPK pathway, suppresses IL-8 production, and reduces the inflammatory response in host cells, thereby enhancing intracellular Salmonella replication [16–18]. In current study, deletion of sptP resulted in induction of strong humoral and cellular immune responses against subsequent Salmonella challenge.
Conclusion
Our present work demonstrates that vaccination of mice with the candidate vaccine C50336ΔsptP conferred development of acquired immunity and efficacious protection against experimental systemic infection. Thus, the sptP mutant strain of S. Enteritidis C50336 has the potential of being a safe, immunogenic vaccine against Salmonella infection.
Abbreviations
CFU:
Colony-forming units
DPI:
Days post-immunization
ELISA:
Enzyme-linked immunosorbent assay
IL-8:
Interleukin-8
LD50
:
50% lethal dose
mLN:
mesenteric lymphadenitis
PBS:
phosphate-buffered saline
S. Enteritidis:
Salmonella enterica serovar Enteritidis
SI:
Stimulation index
SPI-1:
Salmonella pathogenicity islands 1
SptP:
Salmonella protein tyrosine phosphatase
T3SS:
Salmonella Type III secretion system
Declarations
Acknowledgments
Not applicable.
Funding
This work was supported by the Projects of International Cooperation and Exchanges NSFC (31320103907); The Special Fund for Agro-Scientific Research in the Public Interest (201403054); The National Key Research and Development Program Special Project (2016YFD0501607); The Program for High-end talents of Yangzhou University; The Graduate Student Research and Innovation Project of Jiangsu Province (KYZZ_0368); The “Six Talent Peaks Program” of Jiangsu Province (NY-028); The Yangzhou University Science and Technology Innovation Team; Priority Academic Development Program of Jiangsu Higher Education Institutions.
Availability of data and materials
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Authors’ contributions
ZP and XJ: the conception and design of the study. ZL, PT, YJ, XK, QL and XX: acquisition of data, analysis and interpretation of data. ZL, PT and JS: wrote the main manuscript text, prepared figures, statistical analysis and revised the manuscript. QL, XX and JS: technical, material support. XJ: final approval of the version to be submitted. All authors read and approved the final manuscript.
Competing interests
The authors declare that they have no competing interests.
Consent for publication
Not applicable.
Ethics approval
The animal experiments were all approved by the Animal Care and Ethics Committee of Yangzhou University, Yangzhou, China (Approval ID: SYXK [Su] 2012–0029).
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Authors’ Affiliations
(1)
Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, MOA Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOE Joint International Research Laboratory of Agriculture and Agri-product Safety, Yangzhou University
(2)
Division of Gastroenterology and Hepatology, College of Medicine, University of Illinois at Chicago
(3)
Center for Comparative Medicine, Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University
(4)
Department of Anatomy and Cell Biology, Rush University Medical Center
References
Chai SJ, White PL, Lathrop SL, Solghan SM, Medus C, McGlinchey BM, et al. Salmonella enterica serotype Enteritidis: increasing incidence of domestically acquired infections. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America. 2012;54(Suppl 5):S488–97.View ArticleGoogle Scholar
Majowicz SE, Musto J, Scallan E, Angulo FJ, Kirk M, O'Brien SJ, et al. International collaboration on enteric disease 'Burden of illness S: the global burden of nontyphoidal Salmonella gastroenteritis. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America. 2010;50(6):882–9.View ArticleGoogle Scholar
Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson M-A, Roy SL, et al. Foodborne illness acquired in the United States—major pathogens. Emerg Infect Dis. 2011;17(1):7–15.View ArticlePubMedPubMed CentralGoogle Scholar
Braden CR. Salmonella enterica serotype Enteritidis and eggs: a national epidemic in the United States. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America. 2006;43(4):512–7.View ArticleGoogle Scholar
Tollefson LM. Margaret Ann: antibiotic use in food animals: controlling the human health impact. J AOAC Int. 2000;83(2):245–54.Google Scholar
Whichard JM, Gay K, Stevenson JE, Joyce KJ, Cooper KL, Omondi M, et al. Human Salmonella and concurrent decreased susceptibility to quinolones and extended-spectrum cephalosporins. Emerg Infect Dis. 2007;13(11):1681–8.View ArticlePubMedPubMed CentralGoogle Scholar
Mastroeni P, Chabalgoity JA, Dunstan SJ, Maskell DJ, Dougan G. Salmonella: immune responses and vaccines. Vet J. 2001;161(2):132–64.View ArticlePubMedGoogle Scholar
Geng S, Jiao X, Barrow P, Pan Z, Chen X. Virulence determinants of Salmonella Gallinarum biovar Pullorum identified by PCR signature-tagged mutagenesis and the spiC mutant as a candidate live attenuated vaccine. Vet Microbiol. 2014;168(2–4):388–94.View ArticlePubMedGoogle Scholar
Haneda T, Okada N, Kikuchi Y, Takagi M, Kurotaki T, Miki T, et al. Evaluation of Salmonella enterica serovar Typhimurium and Choleraesuis slyA mutant strains for use in live attenuated oral vaccines. Comp Immunol Microbiol Infect Dis. 2011;34(5):399–409.View ArticlePubMedGoogle Scholar
Bohez L, Ducatelle R, Pasmans F, Haesebrouck F, Van Immerseel F. Long-term colonisation-inhibition studies to protect broilers against colonisation with Salmonella Enteritidis, using Salmonella Pathogenicity Island 1 and 2 mutants. Vaccine. 2007;25(21):4235–43.View ArticlePubMedGoogle Scholar
Karasova D, Sebkova A, Vrbas V, Havlickova H, Sisak F, Rychlik I. Comparative analysis of Salmonella enterica serovar Enteritidis mutants with a vaccine potential. Vaccine. 2009;27(38):5265–70.View ArticlePubMedGoogle Scholar
Si W, Wang X, Liu H, Yu S, Li Z, Chen L, et al. Physiology, pathogenicity and immunogenicity of live, attenuated Salmonella enterica serovar Enteritidis mutants in chicks. Microb Pathog. 2015;83-84:6–11.View ArticlePubMedGoogle Scholar
Araya DV, Quiroz TS, Tobar HE, Lizana RJ, Quezada CP, Santiviago CA, et al. Deletion of a prophage-like element causes attenuation of Salmonella enterica serovar Enteritidis and promotes protective immunity. Vaccine. 2010;28(33):5458–66.View ArticlePubMedGoogle Scholar
Fu Y, Galan JE. A Salmonella protein antagonizes Rac-1 and Cdc42 to mediate host-cell recovery after bacterial invasion. Nature. 1999;401(6750):293–7.View ArticlePubMedGoogle Scholar
Murli S, Watson RO, Galan JE. Role of tyrosine kinases and the tyrosine phosphatase SptP in the interaction of Salmonella with host cells. Cell Microbiol. 2001;3(12):795–810.View ArticlePubMedGoogle Scholar
Haraga A, Miller SI. A Salmonella enterica Serovar Typhimurium translocated leucine-rich repeat effector protein inhibits NF- B-dependent Gene expression. Infect Immun. 2003;71(7):4052–8.View ArticlePubMedPubMed CentralGoogle Scholar
Lin SL, Le TX, Cowen DS. SptP, a Salmonella Typhimurium type III-secreted protein, inhibits the mitogen-activated protein kinase pathway by inhibiting Raf activation. Cell Microbiol. 2003;5(4):267–75.View ArticlePubMedGoogle Scholar
Choi HW, Brooking-Dixon R, Neupane S, Lee CJ, Miao EA, Staats HF, et al. Salmonella Typhimurium impedes innate immunity with a mast-cell-suppressing protein tyrosine phosphatase. SptP Immunity. 2013;39(6):1108–20.View ArticlePubMedGoogle Scholar
Datsenko KA, Wanner BL. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A. 2000;97(12):6640–5.View ArticlePubMedPubMed CentralGoogle Scholar
McCormick BA. Salmonella Typhimurium attachment to human intestinal epithelial monolayers: transcellular signalling to subepithelial neutrophils. J Cell Biol. 1993;123(4):895–907.View ArticlePubMedGoogle Scholar
Zhang X, Kelly SM, Bollen WS, Curtiss R 3rd. Characterization and immunogenicity of Salmonella Typhimurium SL1344 and UK-1 delta crp and delta cdt deletion mutants. Infect Immun. 1997;65(12):5381–7.PubMedPubMed CentralGoogle Scholar
Gilles HJ. Calculation of the index of acute toxicity by the method of linear regression. Comparison with the method of "Karber and Behrens". European journal of toxicology and environmental hygiene Journal europeen de toxicologie. 1974;7(2):77–84.PubMedGoogle Scholar
Meng X, Meng X, Zhu C, Wang H, Wang J, Nie J, et al. The RNA chaperone Hfq regulates expression of fimbrial-related genes and virulence of Salmonella enterica serovar Enteritidis. FEMS Microbiol Lett. 2013;346(2):90–6.View ArticlePubMedGoogle Scholar
Rana N, Kulshreshtha RC. Cell-mediated and humoral immune responses to a virulent plasmid-cured mutant strain of Salmonella enterica serotype gallinarum in broiler chickens. Vet Microbiol. 2006;115(1–3):156–62.View ArticlePubMedGoogle Scholar
Brito JR, Hinton M, Stokes CR, Pearson GR. The humoral and cell mediated immune response of young chicks to Salmonella Typhimurium and S. Kedougou. The British veterinary journal. 1993;149(3):225–34.View ArticlePubMedGoogle Scholar
Strugnell RA, Scott TA, Wang N, Yang CY, Peres N, Bedoui S, et al. Salmonella vaccines: lessons from the mouse model or bad teaching? Curr Opin Microbiol. 2014;17(17C):99–105.Google Scholar
Morpeth SC, Ramadhani HO, Crump JA. Invasive non-Typhi Salmonella disease in Africa. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America. 2009;49(4):606–11.View ArticleGoogle Scholar
Katsenos C, Anastasopoulos N, Patrani M, Mandragos C. Salmonella Enteritidis meningitis in a first time diagnosed AIDS patient: case report. Cases journal. 2008;1(1):5.View ArticlePubMedPubMed CentralGoogle Scholar
Gordon MA, Graham SM, Walsh AL, Wilson L, Phiri A, Molyneux E, et al. Epidemics of invasive Salmonella enterica serovar Enteritidis and S. Enterica Serovar Typhimurium infection associated with multidrug resistance among adults and children in Malawi. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America. 2008;46(7):963–9.View ArticleGoogle Scholar
Tena D, Gonzalez-Praetorius A, Bisquert J. Urinary tract infection due to non-typhoidal Salmonella: report of 19 cases. The Journal of infection. 2007;54(3):245–9.View ArticlePubMedGoogle Scholar
Kobayashi H, Hall GS, Tuohy MJ, Knothe U, Procop GW, Bauer TW. Bilateral periprosthetic joint infection caused by Salmonella enterica serotype Enteritidis, and identification of Salmonella sp using molecular techniques. International journal of infectious diseases: IJID: official publication of the International Society for Infectious Diseases. 2009;13(6):e463–6.View ArticleGoogle Scholar
Mutlu H, Babar J, Maggiore PR: Extensive Salmonella Enteritidis endocarditis involving mitral, tricuspid valves, aortic root and right ventricular wall. Journal of the American Society of Echocardiography: official publication of the American Society of Echocardiography 2009, 22(2):210. e1–3.Google Scholar
Ghosh TS, Vogt RL. Cluster of invasive salmonellosis cases in a federal prison in Colorado. Am J Infect Control. 2006;34(6):348–50.View ArticlePubMedGoogle Scholar
Khan SA, Stratford R, Wu T, Mckelvie N, Bellaby T, Hindle Z, et al. Salmonella Typhi and S-Typhimurium derivatives harbouring deletions in aromatic biosynthesis and Salmonella Pathogenicity Island-2 (SPI-2) genes as vaccines and vectors. Vaccine. 2003;21(5–6):538–48.View ArticlePubMedGoogle Scholar
Coombes BK, Coburn BA, Potter AA, Gomis S, Mirakhur K, Li Y, et al. Analysis of the contribution of Salmonella pathogenicity islands 1 and 2 to enteric disease progression using a novel bovine ileal loop model and a murine model of infectious enterocolitis. Infect Immun. 2005;73(11):7161–9.View ArticlePubMedPubMed CentralGoogle Scholar
Boyen F, Pasmans F, Van Immerseel F, Morgan E, Botteldoorn N, Heyndrickx M, et al. A limited role for SsrA/B in persistent Salmonella Typhimurium infections in pigs. Vet Microbiol. 2008;128(3–4):364–73.View ArticlePubMedGoogle Scholar
Dieye Y, Ameiss K, Mellata M, Curtiss R 3rd. The Salmonella Pathogenicity Island (SPI) 1 contributes more than SPI2 to the colonization of the chicken by Salmonella enterica serovar Typhimurium. BMC Microbiol. 2009;9(1):1–14.Google Scholar
Karasova D, Sebkova A, Havlickova H, Sisak F, Volf J, Faldyna M, et al. Influence of 5 major Salmonella pathogenicity islands on NK cell depletion in mice infected with Salmonella enterica serovar Enteritidis. BMC Microbiol. 2010;10:75.View ArticlePubMedPubMed CentralGoogle Scholar
Matulova M, Havlickova H, Sisak F, Rychlik I. Vaccination of chickens with Salmonella Pathogenicity Island (SPI) 1 and SPI2 defective mutants of Salmonella enterica serovar Enteritidis. Vaccine. 2012;30(12):2090–7.View ArticlePubMedGoogle Scholar
Collins FM, Scott MT. Effect of Corynebacterium Parvum treatment on the growth of Salmonella Enteritidis in mice. Infect Immun. 1974;9(5):863–9.PubMedPubMed CentralGoogle Scholar
Berndt A, Wilhelm A, Jugert C, Pieper J, Sachse K, Methner U. Chicken cecum immune response to Salmonella enterica serovars of different levels of invasiveness. Infect Immun. 2007;75(12):5993–6007.View ArticlePubMedPubMed CentralGoogle Scholar
Haraga A, Ohlson MB, Miller SI. Salmonellae interplay with host cells. Nat Rev Microbiol. 2008;6(1):53–66.View ArticlePubMedGoogle Scholar
Cirillo DM, Valdivia RH, Monack DM, Falkow S. Macrophage-dependent induction of the Salmonella pathogenicity island 2 type III secretion system and its role in intracellular survival. Mol Microbiol. 1998;30(1):175–88.View ArticlePubMedGoogle Scholar
