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Table 2 List of primers used for PCR detection, sequencing and qRT-PCR assays

From: Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock

Gene target Sequence (5′-3′) Melting temp. (°C) Amplicon length (bp)
16S rDNA F: ACT AAC GCT GGC GGC GCG
R: TAC CCA CGC TTT CGT GCC
56 1500
secY (1) F: GCG ATT CAG TTT AAT CCT G
R: GCG ATT CAG TTT AAT CCT GC
58 245
ORF22 F: ATT ATT GAG ACA GAG ATA
R: AAG CGG AAC GGG ACG AAT
56 561
ORF36 F: AGT CGG GGT TCG ATA CTG
R: GGT CAG TCC TGT AAC TGC
54 489
IS1500a F: TAC GGA GCA AGA ACG GTT
R: AGA TCC GAT TCT TAT GAT TC
56 143
IS1533a F: TTG TAA TCC CTG TGT TGT TT
R: TTA AAT AGT CCA CTC CTC G
56 130
secY (2)a F: CTG AAT CGC TGT ATA AAA GT
R: GAA GGC TGG TAA ACA AAA G
58 130
  1. afor qRT-PCR