Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock

Table 2 List of primers used for PCR detection, sequencing and qRT-PCR assays

Gene target	Sequence (5′-3′)	Melting temp. (°C)	Amplicon length (bp)
16S rDNA	F: ACT AAC GCT GGC GGC GCG R: TAC CCA CGC TTT CGT GCC	56	1500
secY (1)	F: GCG ATT CAG TTT AAT CCT G R: GCG ATT CAG TTT AAT CCT GC	58	245
ORF22	F: ATT ATT GAG ACA GAG ATA R: AAG CGG AAC GGG ACG AAT	56	561
ORF36	F: AGT CGG GGT TCG ATA CTG R: GGT CAG TCC TGT AAC TGC	54	489
IS1500^a	F: TAC GGA GCA AGA ACG GTT R: AGA TCC GAT TCT TAT GAT TC	56	143
IS1533^a	F: TTG TAA TCC CTG TGT TGT TT R: TTA AAT AGT CCA CTC CTC G	56	130
secY (2)^a	F: CTG AAT CGC TGT ATA AAA GT R: GAA GGC TGG TAA ACA AAA G	58	130

ISSN: 1746-6148