Table 3 Primer and probe sequences for RT-PCR-based detection of BVDV
ID | Sequence (5’ - 3’) | Size (bp) | Amplicon size (bp) | Region amplified | Target viruses | Source |
|---|---|---|---|---|---|---|
Pesti-FW | 5’-CTAGCCATGCCCTTAGTAG-3’ | 19 | 106 | 5’-UTR | Common for Pestivirus | [17] |
Pesti-RS | 5’-CGTCGAACCAGTGACGACT-3’ | 19 | ||||
FAM-BVDV1 | 5’-FAM-TAGCAACAGTGGTGAGTTCGTTGGATGGCT-BHQ-3’ | 30 | BVDV-1 |