First report of junctional epidermolysis bullosa (JEB) in the Italian draft horse
© Cappelli et al.; licensee BioMed Central. 2015
Received: 2 July 2014
Accepted: 24 February 2015
Published: 10 March 2015
Epitheliogenesis imperfecta in horses was first recognized at the beginning of the 20th century when it was proposed that the disease could have a genetic cause and an autosomal recessive inheritance pattern. Electron microscopy studies confirmed that the lesions were characterized by a defect in the lamina propria and the disease was therefore reclassified as epidermolysis bullosa. Molecular studies targeted two mutations affecting genes involved in dermal–epidermal junction: an insertion in LAMC2 in Belgians and other draft breeds and one large deletion in LAMA3 in American Saddlebred.
A mechanobullous disease was suspected in a newborn, Italian draft horse foal, which presented with multifocal to coalescing erosions and ulceration on the distal extremities. Histological examination of skin biopsies revealed a subepidermal cleft formation and transmission electron microscopy demonstrated that the lamina densa of the basement membrane remained attached to the dermis. According to clinical, histological and ultrastructural findings, a diagnosis of junctional epidermolysis bullosa (JEB) was made. Genetic tests confirmed the presence of 1368insC in LAMC2 in the foal and its relatives.
This is the first report of JEB in Italy. The disease was characterized by typical macroscopic, histologic and ultrastructural findings. Genetic tests confirmed the presence of the 1368insC in LAMC2 in this case: further investigations are required to assess if the mutation could be present at a low frequency in the Italian draft horse population. Atypical breeding practices are responsible in this case and played a role as odds enhancer for unfavourable alleles. Identification of carriers is fundamental in order to prevent economic loss for the horse industry.
KeywordsJunctional epidermolysis bullosa Horse Mechanobullous disease Electron microscopy Lamina densa LAMC2 Italian draft horse Inbreeding
Junctional epidermolysis bullosa (JEB) belongs to the group of vesiculo-bullous diseases of the epidermis. With this term, several diseases are encompassed that are all characterized by the formation of a split (vesicle or bulla) in any layer of the epidermis or beneath it, at the dermoepidermal junction. This split occurs in two ways: as a consequence of an immune-mediated attack to components of the intercellular and cell-basement membrane adhesion system or as a result of an inherited condition resulting in a lack of any of these components. In this second case, epidermolysis bullosa (EB) is a recessive inherited disease characterized by a genetic defect leading to an inadequate synthesis of structural components of intercellular adhesions such as keratin filaments, desmosomes and hemi-desmosome proteins and anchoring fibrils such as collagen VII . In humans, three subtypes of EB are described that are classified according to the distribution of the lesions and the location of the split in the epidermis and dermis: in simplex EB, the split forms in the basal keratinocyte layer; in junctional EB the split forms in the lamina lucida, leaving the lamina densa anchored to the underlying dermis; in dystrophic EB the split forms within or below the lamina densa which therefore remains attached to the overlying epidermis .
Epidermolysis bullosa is recognized in dogs, sheep, horses, cattle, goats and cats [3-7]. Lesions can be present at birth or develop in a short period of time and are characterized by the development of vesicles and bullae that rapidly progress to erosions and ulceration at sites of minor trauma such as the lips, the oral mucosa, the distal extremities and the coronary band, with resulting sloughing of the hoof or claws. Lesions can be secondarily infected and become pustules. Affected animals may die soon after birth due to inability to suckle. Histologically, the lesions show a split that can be intraepidermal, at the dermoepidermal junction or subepidermal. The anatomical location of the split is an important diagnostic criterion because it reflects a different pathogenesis of lesion formation .
In horses, two mutations have been associated with the disease, involving two different genes coding for the Laminin 332 protein complex [4,8]. Laminin is a heterotrimeric basement membrane protein integral to the structure and function of the dermal–epidermal junction consisting of three glycoprotein subunits: the α3, β3 and γ2 chains, which are encoded by the LAMA3, LAMB3 and LAMC2 respectively . A mutation in any of these genes results in the condition known as hereditary junctional epidermolysis bullosa (JEB). An insertion of a cytosine (1368insC) in the LAMC2 was found in 2002 in draft horses (Belgian Horse, Trait Breton and Trait Comtois) [4,10]. This mutation is responsible for a frame-shift, with consequent premature stop codon formation, leading to a truncated form of the Laminin 332 chain. In 2009, a 6589-bp deletion spanning exons 24 and 27 was found in the LAMA3 in American Saddlebred foals born with the skin-blistering condition formerly known as epitheliogenesis imperfecta. The deletion confirms that the disease can be classified as JEB and corresponds to Herlitz JEB in humans . In both cases, the inheritance of the disease is a classic Mendelian autosomal recessive.
These results confirmed that the mutation causing junctional epidermolysis bullosa in the foal was localized in the LAMC2, as already described in northern Europe’s coldblood breeds (Belgian Horse, Trait Breton and Trait Comtois) [4,10], which participated, with some lines, to the creation of Italian draft horses ; since the disease has a classical autosomal recessive Mendelian inheritance, both parents must be heterozygous (carriers).
Inbreeding, enhanced by erroneous breeding practices, should always be avoided as it can increase the frequency of potentially deleterious recessive alleles in the population and their phenotypic manifestation at individual level .
This is the first report of JEB in Italy. The disease was histologically described as having the typical pathognomonic features and assessed via molecular tests.
Future studies should include genotyping the 1368insC mutation in LAMC2 in a larger population of Italian Draft horses to determine the allele frequency within this population, and avoid other episodes of JEB.
Identification of carriers is crucial as much as breeder awareness about the avoidance of certain mates in order to prevent economic loss for the horse industry.
The authors would like to acknowledge Gianluca Alunni for his technical help and Paola Coliolo for her excellent work in the preparation of the ultrastructural samples.
- Intong LRA, Murrell DF. Inherited epidermolysis bullosa: New diagnostic criteria and classification. Clin Dermatol. 2012;30:70–7 [Bullous Skin Diseases: Part II].View ArticlePubMedGoogle Scholar
- Sawamura D, Nakano H, Matsuzaki Y. Overview of epidermolysis bullosa. J Dermatol. 2010;37:214–9.View ArticlePubMedGoogle Scholar
- Scott D, Miller W. Congenital and Hereditary Skin Diseases. 2nd Edition. Saunders; 2011.Google Scholar
- Spirito F, Charlesworth A, Linder K, Ortonne J-P, Baird J, Meneguzzi G. Animal models for skin blistering conditions: absence of laminin 5 causes hereditary junctional mechanobullous disease in the Belgian horse. J Investig Dermatol. 2002;119:684–91.View ArticlePubMedGoogle Scholar
- Kerkmann A, Ganter M, Frase R, Ostmeier M, Hewicker-Trautwein M, Distl O. Epidermolysis bullosa in German black headed mutton sheep. Berl Munch Tierarztl Wochenschr. 2010;123:413–21.PubMedGoogle Scholar
- Medeiros GX, Riet-Correa F, Armién AG, Dantas AF, de Galiza GJ, Simões SV. Junctional epidermolysis bullosa in a calf. J Vet Diagn Invest. 2012;24:231–4.View ArticlePubMedGoogle Scholar
- Medeiros GX, Riet-Correa F, Barros SS, Soares MP, Dantas AF, Galiza GJ, et al. Dystrophic epidermolysis bullosa in goats. J Comp Pathol. 2013;148:354–60.View ArticlePubMedGoogle Scholar
- Graves KT, Henney PJ, Ennis RB. Partial deletion of the LAMA3 gene is responsible for hereditary junctional epidermolysis bullosa in the American Saddlebred Horse. Anim Genet. 2009;40:35–41.View ArticlePubMedGoogle Scholar
- Finno CJ, Spier SJ, Valberg SJ. Equine diseases caused by known genetic mutations. Vet J. 2009;179:336–47.View ArticlePubMedGoogle Scholar
- Milenkovic D, Chaffaux S, Taourit S, Guérin G. A mutation in the LAMC2 gene causes the Herlitz junctional epidermolysis bullosa (H-JEB) in two French draft horse breeds. Genet Sel Evol. 2003;35:249–56.View ArticlePubMed CentralPubMedGoogle Scholar
- Hendricks B. International Encyclopedia of Horse Breeds. University of Oklahoma Press; 2007.Google Scholar
- Hartl DL, Clark AG. Principles of population genetics. 4th ed. Sunderland, Massachusetts: Sinauer Associates, Inc. Publishers; 2007.Google Scholar
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.