Table 1 The designed shRNA sequences of the bovine CD14 gene
From: Establishment of a mouse model to express bovine CD14 short hairpin RNA
Name | Duplexes of DNA coding specific shRNA (5’-3’) |
|---|---|
shRNA-674 | S 5 ’-GCCTAGACCTGTCTGACAATTTCAAGAGAATTGTCAGACAGGTCTAGGC-3’ |
AS 5’-CGGATCTGGACAGACTGTTAAAGTTCTCTTAACAGTCTGTCCAGATCCG-3’ | |
shRNA-279 | S 5’-GCCTGGAACAGTTTCTCAAGGTTCAAGAGACCTTGAGAAACTGTTCCAGGC-3’ |
AS 5’-CGGACCTTGTCAAAGAGTTCCAAGTTCTCTGGAACTCTTTGACAAGGTCCG-3’ | |
shRNA-326 | S 5’-GCTGACACAATCAAGGCTCTGTTCAAGAGACAGAGCCTTGATTGTGTCAGC-3’ |
AS 5’-CGACTGTGTTAGTTCCGAGACAAGTTCTCTGTCTGGCAACTAACACAGTCG-3’ | |
Scrambled shRNA-1864 | S 5’-CTCGAGCCGGCCTAAGGTTAAGTCGCCCTCGCTCG AGCGAGGGCGACTTAACCTTAGGTTTTTTGGCGGCCGC-3’ |
AS 5’-GCGGCCGCCAAAAAACCTAAGGTTAAGTCGCCCTC GCTCGAGCGAGGGCGACTTAACCTTAGGCCGGCTCGAG-3’ |