- Research article
- Open Access
Detection of Toxoplasma gondiioocysts in soils in Northwestern China using a new semi-nested PCR assay
BMC Veterinary Research volume 10, Article number: 238 (2014)
Toxoplasma gondii is a zoonotic pathogen that can infect a range of animals and humans. Ingestion of T. gondii oocysts in soil is a significant transmission route for humans and animals acquiring toxoplasmosis. In the present study, we developed a new semi-nested PCR method to determine T. gondii oocysts distribution in soils in northwestern China.
The one tube semi-nested PCR assay was developed to detect the oocysts of T. gondii in soil, targeting the repetitive 529 bp fragment of T. gondii genomic DNA. Then a total of 268 soil samples, including 148 samples from Gansu Province and 120 samples from Qinghai Province, northwestern China, were examined by the semi-nested PCR method. One third of the positive samples were sequenced. The sensitivity of the semi-nested PCR assay was 102 T. gondii oocysts in 5 g soil sample. Investigation of soil samples from northwestern China showed that 34 out of 268 soil samples (12.69%) were T. gondii positive. Sequences of the partial 529 bp fragments varied from 0-1.2% among the sequenced samples. The prevalence of T. gondii oocysts in soil from cities (24/163) was slightly higher than that in soils from pasturing areas (10/105) (P = 0.21). Among the different regions in cities, the prevalence of T. gondii oocysts in soils from parks was 14.15%, whereas that in soils from schools was 19.05%.
The present study firstly reported the prevalence of T. gondii oocysts in soils in northwest China using a novel semi-nested PCR assay, which provided baseline data for the effective prevention and control of toxoplasmosis in this region.
Toxoplasmosis caused by Toxoplasma gondii is a zoonotic infection of human beings and animals -. The parasite can lead to severe disease or even death in immunocompromised hosts such as AIDS patients, organ transplant recipients and malignancy patients and can also result in abortion, stillbirth or other serious consequences in newborns when the infection occurs in pregnant women ,,. T. gondii infection in livestock usually causes abortions, stillbirths, and neonatal deaths, especially in sheep and goats, which have been leading to serious economic losses worldwide ,.
The sporulated oocysts of T. gondii are resistant to harsh climatic circumstances ,. The moist condition can prolong the survival time to more than a year. Ingestion of soil, food or water contaminated by sporulated T. gondii oocysts after shedding from felids is considered significant routes of T. gondii transmission to humans and animals . Infections through oocysts have been widely reported in China and many other countries ,-. Thereinto, exposure to contaminated soil is identified as a strong risk factor revealed by studies from Europe and USA, particularly high for children -. The contaminated soil may also transfer oocysts to vegetables and fruits for human consumption, which increase risks of primary infection ,.
Due to the important role of contamination of soil in the transmission dynamics of T. gondii, previous studies developed several methods such as loop-mediated isothermal amplification (LAMP) and QT-PCR to investigate its oocysts in soils -. However, no information about the T. gondii oocysts prevalence in soils could be available in northwestern China, especially in the pasturing areas. The objective of the present study was to investigate prevalence of T. gondii oocysts in the soil samples from Qinghai and Gansu provinces in northwest China. Due to the low concentration of T. gondii contained in soils, we developed a new semi-PCR method based on the 529-bp repeat element to improve the sensitivity.
This study was approved by the Ethics Committee of Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (Approval No. LVRIAEC2012-018), and the soil samples were collected strictly according to the requirements of the Ethics Procedures and Guidelines of the People's Republic of China.
The investigated site
The present study was carried out in Qinghai province (31°-39°N, 88°-103°E) and Gansu province (34.6°-36.73°N, 101.94°-108.43°), which lie on the northwestern of People's Republic of China. The average elevation of Qinghai province is 3 000 m above sea level, and Gansu province is more than 1 000 m above sea level. The annual precipitation of the survey regions are below 500 mm and average annual temperature are between -4.3°C and 14.8°C. The survey regions belong to typical continental climate.
148 soil samples from 5 schools and 2 parks were collected in Lanzhou city, and the other 120 samples were collected from Huzhu, Ping’an,, Huangyuan, Xining, Haibei and Hainan cities in Qinghai Province. Thereinto, Xining and Lanzhou cities are capitals of Qinghai and Gansu Provinces, respectively, and the rest 5 cities locate in the pasturing areas.
T. gondii oocysts cultivation and DNA extraction
Three captive-bred cats (three-month old) were inoculated T. gondii GJS strain orally via a stomach tube. One week after the challenge, the T. gondii oocysts were isolated from feces and were purified using differential centrifugation according to Haydee et al. . The sediments were kept in 2% sulfuric acid at room temperature to form sporulated oocysts, and then stored at 4°C until further analysis. The genomic DNA from soil samples were extracted using the stool DNA kit (Omega Bio-Tek Inc, USA) according to the manufacturer's instructions.
Development of the semi-nested PCR
To detect T. gondii oocysts, the specific semi-nested PCR reaction was developed targeting the T. gondii 529-bp fragment (accession number: AF146527). The length of targeting fragment to design the primers was 344 bp, with the primers of TX1: 5′-CAGGGAGGAAGACGAAAGTTG-3', TX2: 5′-CACAGAAGGGACAGAAGT-3', TX3: 5'-CTGTGTCACGTAGACCTAAGG-3'. The amplification mixture consisted of 12.5 μl of 2 × reaction mix (PCR Premix Tag, Takara), 0.004 μM of TX1, 0.2 μM of TX2, 0.2 μM of TX3, 0.5 μl of 1% BSA, and 1 μl of soil template DNA in a final volume of 25 μl. The reaction mixture was initially incubated for 3 min at 95°C to denature the template DNA, and then samples were amplified as follows: 15°Cycles of denaturation at 95°C for 30's and annealing/extension at 64°C for 40's, extension at 72°C for 5 min, 35°Cycles of denaturation at 95°C for 30's and annealing/extension at 55°C for 40's, extension at 72°C for 8 min. Semi-nested PCR product was subjected to electrophoresis on a 2.0% agarose gel in a Tris-acetic acid-EDTA (TAE) buffer at 80 V for 30 min and visualized under UV light after staining with ethidium bromide.
Experiment soil sample recovery and soil sample DNA extraction
Seven oocyst-free soil samples (5 g) were prepared in a laboratory and experimentally contaminated with oocysts in the following amounts: 106, 105, 104, 103, 102, 101, and 100. Every soil sample (including experimental and field samples) was filtered by double gauzes, and then genomic DNA of T. gondii from soil samples were extracted from 0.4 g soil using the soil DNA kit (Omega Bio-Tek Inc, USA) according to the manufacturer's instructions. The DNA was eluted in 70 μl elution buffer and stored at -20°C until further analysis.
Application of semi-nested PCR for soil sample
To evaluate the feasibility of the semi-nested PCR assay for the analysis of field samples, a total of 268 field soil samples were collected from Gansu and Qinghai provinces, China. The genomic DNA of T. gondii oocysts were isolated as described above. The samples were subjected to the developed semi-nested PCR to detect T. gondii oocysts in soil. Each sample was performed in triplicate. Ten of the positive samples were randomly collected and then sent to Sangon Biotech Co. Ltd. (China) for sequencing.
The difference in the prevalent rates of T. gondii between regions in soil samples were analyzed by Chi-square analysis in SAS (Statistical Analysis System, Version 8.0). Values of P < 0.05 were considered as statistically significant.
The sensitive detection of the semi-nested PCR assay was determined by 10-fold serial dilution of the oocysts amount. The result showed that the method could detect the limitation of 102 T. gondii oocysts in 5 g soil sample (Figure 1).
As shown in Table 1, 34 out of 268 soil samples (12.69%) were detected T. gondii oocysts positive using the semi-nested PCR method. The sequencing results indicated that the 10 amplicons were homologous to 529-fragment of T. gondii and a lot of 4 variable nucleotide positions were identified in the obtained sequences, ranging from 0-1.2%, which included 1 transtion (C < − > A) and 3 transversions (A < - > T and C < - > G). The prevalence of T. gondii oocysts in soils from cities (24/163) was higher than that from pasturing areas (10/105), and the difference was not statistically significant (P = 0.21). Among the different regions in Lanzhou city, the prevalence in soils from schools was 14.15% (15/106), which was statistically not significantly different to that in soils from parks (8/42, P = 0.66) (Table 2).
Soil is a significant environmental source of T. gondii infection in humans and animals ,. A previous study detected that 18 out of 101 soil samples were performed positive against T. gondii oocysts in environmental soil samples in Poland using PCR method based on the B1 gene . In that study, the parasite contamination of the soil sample with at least 103 oocysts for 40 g soil could be detected . Another study showed that the detection limit of PCR method using 529-fragment was 5 tachyzoites in soil similar with that of loop-mediated isothermal amplification (LAMP) method, which the sensitivity was higher than the PCR method using B1 gene ,. Edvinsson et al.  also detected the 529 bp repeat element gave better sensitivity than the B1 gene at low concentrations of T. gondii DNA. Thus, the 529 bp repeat element was usually used as target molecular to develop detection method of T. gondii-. In the present study, we developed a new semi-nested PCR method to detect T. gondii oocysts in soils based on the 529 bp repeat fragment, which was more sensitive than the PCR amplification of B1 gene . To ensure the validity of our investigation, one third of the positive samples were chosen to sequence.
A total of 268 soil samples from northwestern China were further applied in epidemiological studies with the semi-nested PCR method. Positive DNA against T. gondii oocysts was detected in 34 (12.69%, 95% CI 8.64-16.55) soil samples. The prevalence of oocysts in soil was lower than that in Hubei province, China by PCR method and in São Paulo, Brazil by immunohistochemistry and indirect fluorescent antibody test (IFAT) ,. The different positive rates in these studies may be due to differences in diagnostic methods used, animal welfare especially the care of cats, as well as the different environments. The investigated places belong to the typical continental monsoon climate with the dry and cold circumstances. Furthermore, the UV radiance in these plateau regions are little stronger than that in the flat areas. These environmental conditions were harsh for the survival of T. gondii oocysts, and were unfavorable for epidemics of toxoplasmosis ,, which was coincided with previous studies that seroprevalence of the parasite in pet animals  and poultry  in Lanzhou city and in free-grazed animals  was lower than that in most of other moist places.
City was >1× μMore than pasturing area to be contaminated T. gondii oocysts (OR 1.64, 95% CI 0.75-3.59). The widespread distribution of T. gondii in city was mainly caused by stray or free-living cats. In our previous study, we isolated 11 T. gondii strains from 41 stray cats , therefore, ubiquitous stray cats infected by T. gondii are contributed to the high prevalence of the parasite and constant infection pressure in the environment . We can also find support for this tendency from the higher prevalence in soil samples from school. The school A and school C were not far from pet animal markets. The residual foods on the ground attract stray cats to eat. The school A and C may become the haunt of stray cats.
The T. gondii prevalence in schools indicates a main risk source of infection for younger, especially the presence in soil from school C and E. The school C and E were elementary schools. Students, sometimes, may play with earth. They may be infected with T. gondii due to blot hands with the oocysts contaminated soil and then eat food without any awareness. So, parents should be take appropriate precautions. In addition, regularly cleaning the surroundings and other integrated strategies and measures are necessary for the effective prevention and control of T. gondii oocysts prevalence.
The present study developed a new semi-nested PCR assay based on the repetitive 529 bp fragment of T. gondii genomic DNA. Using this assay, high level (12.69%) contamination of T. gondii oocysts in soils in northwestern China was revealed for the first time, indicating a potential risk of soil as an important source of T. gondii infection in humans and other animals in this region of China.
Dubey JP, Beattie CP: Toxoplasmosis of animals and humans. CRC Press Inc, Boca Raton, Florida: 2010.
Montoya JG, Liesenfeld O: Toxoplasmosis. Lancet. 2004, 363: 1965-1976. 10.1016/S0140-6736(04)16412-X.
Yuan ZG, Luo SJ, Dubey JP, Zhou DH, Zhu YP, He XH, Zhang XX, Zhu XQ: Serological evidence of Toxoplasma gondii infection in five species of bats in China. Vector Borne Zoonotic Dis. 2013, 13: 422-424. 10.1089/vbz.2012.1091.
Robert-Gangneux F, Darde ML: Epidemiology of and diagnostic strategies for toxoplasmosis. Clin Microbiol Rev. 2012, 25: 264-296. 10.1128/CMR.05013-11.
Tian YM, Dai FY, Huang SY, Deng ZH, Duan G, Zhou DH, Yang JF, Weng YB, Zhu XQ, Zou FC: First report ofToxoplasma gondiiseroprevalence in peafowls in Yunnan Province, Southwestern China.Parasit Vectors 2012, 5:205..
Nowakowska D, Wujcicka W, Sobala W, Spiewak E, Gai Z, Wilczyński J: Age-associated prevalence of Toxoplasma gondii in 8281 pregnant women in Poland between 2004 and 2012. Epidemiol Infect. 2014, 142: 656-661. 10.1017/S0950268813001179.
Zhou P, Chen Z, Li HL, Zheng H, He S, Lin RQ, Zhu XQ: Toxoplasma gondiiinfection in humans in China.Parasit Vectors 2011, 4:165..
Elmore SA, Jones JL, Conrad PA, Patton S, Lindsay DS, Dubey JP: Toxoplasma gondii: Epidemiology, feline clinical aspects, and prevention. Trends Parasitol. 2010, 26: 190-196. 10.1016/j.pt.2010.01.009.
Aramini JJ, Stephen C, Dubey JP, Engelstoft C, Schwantje H, Ribble CS: Potential contamination of drinking water with Toxoplasma gondii oocysts. Epidemiol Infect. 1999, 122: 305-315. 10.1017/S0950268899002113.
Balasundaram MB, Andavar R, Palaniswamy M, Venkatapathy N: Outbreak of acquired ocular toxoplasmosis involving 248 patients. Arch Ophthalmol. 2010, 128: 28-32. 10.1001/archophthalmol.2009.354.
de Moura L, Bahia-Oliveria LM, Wada MY, Jones JL, Tuboi SH, Carmo EH, Ramalho WM, Camargo NJ, Trevisan R, Graca RM, da Silva AJ, Moura I, Dubey JP, Garrett DO: Waterborne toxoplasmosis, Brazil, fromfield to gene. Emerg Infect Dis. 2006, 12: 326-329. 10.3201/eid1202.041115.
Cook AJ, Gilbert RE, Buffolano W, Zufferey J, Petersen E, Jenum PA, Foulon W, Semprini AE, Dunn DT: Sources of Toxoplasma infection in pregnant women: European multicentre case°Control study. BMJ. 2000, 321: 142-147. 10.1136/bmj.321.7254.142.
dos Santos TR, Nunes CM, Luvizotto MC, de Moura AB, Lopes WD, da Costa AJ, Bresciani KD: Detection of Toxoplasma gondii oocysts in environmental samples from public schools. Vet Parasitol. 2010, 171: 53-57. 10.1016/j.vetpar.2010.02.045.
Jones JL, Kruszon-Moran D, Won K, Wilson M, Schantz PM: Toxoplasma gondii and Toxocara spp. co-infection. Am J Trop Med Hyg. 2008, 78: 35-39.
Berger F, Goulet V, Le Strat Y, Desenclos JC: Toxoplasmosis among pregnant women in France: risk factors and change of prevalence between 1995 and 2003. Rev Epidemiol Sante Publique. 2009, 57: 241-248. 10.1016/j.respe.2009.03.006.
Kapperud G, Jenum PA, Stray-Pedersen B, Melby KK, Esdild A, Eng J: Risk factors for Toxoplasma gondii infection in pregnancy. Results of a prospective case°Control study in Norway. Am J Epidemiol. 1996, 144: 405-412. 10.1093/oxfordjournals.aje.a008942.
Lass A, Pietkiweicz H, Modzelewska E, Dumètre A, Szostakowska B, Myjak P: Detection of Toxoplasma gondii oocysts in environmental soil samples using molecular methods. Eur J Clin Microbiol Infect Dis. 2009, 28: 599-605. 10.1007/s10096-008-0681-5.
Dabritz HA, Miller MA, Atwill ER, Gardner IA, Leutenegger CM, Melli AC, Conrad PA: Detection of Toxoplasma gondii-like oocysts in cat feces and estimates of the environmental oocyst burden. J Am Vet Med Assoc. 2007, 231: 1676-1684. 10.2460/javma.231.11.1676.
Du F, Feng HL, Nie H, Tu P, Zhang QL, Hu M, Zhou YQ, Zhao JL: Survey on the contamination of Toxoplasma gondii oocysts in the soil of public parks of Wuhan, China. Vet Parasitol. 2012, 184: 141-146. 10.1016/j.vetpar.2011.08.025.
Noqui FL, Mattas S, Turcato Júnior g, Lewi DS: Neurotoxoplasmaosis diagnosis for HIV-1 patients by real-time PCR of cerebrospinal fluid. Braz J Infect Dis. 2009, 13: 18-23. 10.1590/S1413-86702009000100006.
Su C, Shwab EK, Zhou P, Zhu XQ, Dubey JP: Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii. Parasitology. 2010, 137: 1-11. 10.1017/S0031182009991065.
Edvinsson B, Lappalainen M, Ecengård B: Real-time PCR targeting a 529-bp repeat element for diagnosis of toxoplasmosis. Clin Microbiol Infect. 2006, 12: 131-136. 10.1111/j.1469-0691.2005.01332.x.
Afonso E, Lemoine M, Poulle ML, Ravat MC, Romand S, Thulliez P, Villena I, Aubert D, Rabilloud M, Riche B, Gilot-Fromont E: Spatial distribution of soil contamination by Toxoplasma gondii in relation to cat defecation behaviour in an urban area. Int J Parasitol. 2008, 38: 1017-1023. 10.1016/j.ijpara.2008.01.004.
Homan WL, Vercammen M, De Braekeleer J, Verschueren H: Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use of diagnostic and quantitative PCR. Int J Parasitol. 2000, 30: 69-75. 10.1016/S0020-7519(99)00170-8.
Gomez-Marin JE, De-La-Torre A, Angel-Muller E, Rubio J, Arenas J, Osorio E, Nuñez L, Pinzon L, Mendez-Cordoba LC, Bustos A, De-la-Hoz I, Silva P, Beltran M, Chacon L, Marrugo M, Manjarres C, Baguero H, Lora F, Torres E, Zuluaga OE, Estrada M, Mosxote L, Silva MT, Rivera R, Molina A, Najera S, Sanabria A, Ramirez ML, Alacon C, Restrepo N, et al: First Colombian multicentric newborn screening for congenital toxoplasmosis.PLoS Negl Trop Dis 2011, 5:e1195..
Afonso E, Thulliez P, Gilot-Fromont E: Local meteorological conditions, dynamics of seroconversion to Toxoplasma gondii in cats (Felis catus) and oocyst burden in a rural environment. Epidemiol Infect. 2010, 138: 1105-1113. 10.1017/S0950268809991270.
Wu SM, Huang SY, Fu BQ, Liu GY, Chen JX, Chen MX, Yuan ZG, Zhou DH, Weng YB, Zhu XQ, Ye DH: Seroprevalence ofToxoplasma gondiiinfection in pet dogs in Lanzhou, Northwest China.Parasit Vectors 2011, 4:64..
Cong W, Huang SY, Zhou DH, Xu MJ, Wu SM, Yan C, Zhao Q, Song HQ, Zhu XQ: First report ofToxoplasma gondiiin infection in market-sold adult chickens, ducks and pigeons in northwest China.Parasit Vectors 2012, 5:110..
Wang M, Wang YH, Ye Q, Meng P, Yin H, Zhang DL: Serological survey ofToxoplasma gondiiin Tibetan mastiffs (Canis lupus familiaris) and yaks (Bos grunniens) in Qinghai, China.Parasite Vectors 2012, 5:35..
Project support was provided by the National Natural Science Foundation of China (Grant Nos. 31230073 and 31172316), and the Science Fund for Creative Research Groups of Gansu Province (Grant No. 1210RJIA006).
The authors declare that they have no competing interests.
DLZ and JXL conceived and designed the study, and critically revised the manuscript. MW, PM, QY, YHP, and XYY performed the experiments. MW, NZZ and DLZ analyzed the data and drafted the manuscript. All authors read and approved the final manuscript.
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.
The Creative Commons Public Domain Dedication waiver (https://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Wang, M., Meng, P., Ye, Q. et al. Detection of Toxoplasma gondiioocysts in soils in Northwestern China using a new semi-nested PCR assay. BMC Vet Res 10, 238 (2014). https://doi.org/10.1186/s12917-014-0238-z
- Toxoplasma gondii
- Semi-nested PCR