Open Access

Erratum to: Urine neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute canine kidney injury

  • Ya-Jane Lee,
  • Yu-Yen Hu,
  • Yi-Shan Lin,
  • Chun-Ting Chang,
  • Fong-Yuan Lin,
  • Min-Liang Wong,
  • Hsu Kuo-Hsuan and
  • Wei-Li Hsu
BMC Veterinary Research20139:228

https://doi.org/10.1186/1746-6148-9-228

Received: 30 October 2013

Accepted: 30 October 2013

Published: 14 November 2013

The original article was published in BMC Veterinary Research 2012 8:248

After the publication of this work [1], we became aware of the fact that the values of NGAL reported in this study were concentrations of diluted clinic samples; and therefore for the actual concentration of urine NGAL, the value should multiply the dilution factor.

With exception of Figure three (Figure 1 here), all the values of urine NGAL described in this study were detected by in-house ELISA and subsequently were converted to the concentrations corresponding to the values detected with a commercial NGAL ELISA kit by the equation of Y = 0.8855X + 6.5587.
Figure 1

A comparison of the results obtained by a commercial ELISA kit and by our NGAL ELISA. (A) The urine NGAL(uNGAL) concentrations of twenty urine samples collected from 10 healthy and 10 renal failure dogs measured by Commercial ELISA kit (left panel) and by our ELISA (right panel). (B) Bland-Altman plot of uNGAL concentrations from 20 urine samples measured by the two methods. (C) Linear regression analysis and coefficient of correlation analysis (Software SigmaPlot 10.0) were conducted to correlate the results of these two ELISA systems. Each column represents the μNGAL concentration of a dog.

As a consequence, for the concentration of NGAL in urine samples listed in Table two (Table 1 here), all the values have to multiply the dilution factor: 100. And the NGAL concentrations described in abstract (subtitle of Results) should be corrected as follows: At 12 h after surgery, compared to the group without AKI (12 dogs), the NGAL level in the urine of seven dogs with AKI was significantly increased (median 17.840 ng/mL vs. 8.8 ng/mL), and this difference was sustained to 72 h.
Table 1

Median/mean of urine and serum NGAL levels as well as serum creatinine levels at various time points

Time point

AKI

No AKI

P a

Urine NGAL (ng/mL)

   

0 h

5.14 (5.21), n = 11

8.39 (21.63), n = 25

0.959

12 h

17.84 ( 24.03), n = 7

8.80 (19.52), n = 12

0.022

24 h

24.36 ( 17.03), n = 10

12.82 (21.85), n = 24

0.059

48 h

20.17 (25.66), n = 11

15.57 (26.18), n = 25

0.035

72 h

27.61(26.96), n = 10

7.0 (25.72), n = 17

0.056

Max after 72 h

29.75 (19.39), n = 11

16.11 (19.93), n = 25

0.041

Serum NGAL (ng/mL)

   

0 h

25.46 ± 3.55, n = 8

21.79 ± 2.20, n = 16

0.368

12 h

17.75 ± 3.38, n = 7

20.94 ± 2.70 n = 13

0.481

24 h

22.68 ± 2.89, n = 12

23.09 ± 2.11, n = 25

0.910

48 h

26.93 ± 3.11, n = 12

22.83 ± 2.34, n = 25

0.313

72 h

25.45 ± 3.81, n = 9

21.75 ± 2.20, n = 18

0.377

Max after 72 h

29.75 ± 2.92, n = 12

25.49 ± 2.24, n = 26

0.277

Serum Creatinine (μmol/L)

   

0 h

79.6 ± 7.1, n = 12

88.4 ± 3.5, n = 27

0.203

12 h

77.8 ± 11.5, n = 6

83.1 ± 3.5, n = 13

0.583

24 h

104.3 ± 8.8, n = 12

85.7 ± 2.7, n = 26

0.040

48 h

103.4 ± 0.1, n = 12

86.6 ± 2.7, n = 26

0.027

72 h

113.2 ± 8.8, n = 10

85.7 ± 2.9, n = 21

0.005

Mean ± S.E.M. and median (IQR) are used to present normally and non-normally distributed continuous data, respectively.

aP < 0.05 indicates a significant difference.

As for Figure three (Figure 1 here), the values directly obtained by the commercial NGAL ELISA and by in-house NGAL ELISA need to multiply the dilution factors 100 or 20, respectively. We already corrected the value in the new Figure three (Figure 1 here).

We regret any inconvenience that this inaccuracy in the data used for the original analysis might have caused. We wish to thank Dr. Evence Daure for bringing this matter to our attention.

Notes

Declarations

References

  1. Lee YJ, Hu YY, Lin YS, Chang CT, Lin FY, Wong ML, Kuo-Hsuan H, Hsu WL: Urine neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute canine kidney injury. BMC Vet Res. 2012, 8: 248-10.1186/1746-6148-8-248.PubMedPubMed CentralView ArticleGoogle Scholar

Copyright

© Lee et al.; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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