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Table 3 Genes and real-time amplification products.

From: Effect of the feeding system on the fatty acid composition, expression of the Δ9-desaturase, Peroxisome Proliferator-Activated Receptor Alpha, Gamma, and Sterol Regulatory Element Binding Protein 1 genes in the semitendinous muscle of light lambs of the Rasa Aragonesa breed

     PCR condition
Target gene Primers: Forward and reverse Amplicon bp Acc. Number AT Nm R2 Slope
SCD F-5'cccagctgtcagagaaaagg-3'
R-5'gatgaagcacaacagcagga-3'
115 AJ001048 59 900
900
0.996 -3.35
SREBP1 F-5' ctgctatgcaggcagcac-3'
R-5' ggttgatgggcagcttgt-3'
99 GU206528 59 900
900
0.980 -3.32
PPARγ F-5'cttgctgtggggatgtctc-3'
R-5'ggtcagcagactctgggttc-3'
121 AY137204 60 900
900
0.982 -3.35
PPARα F-5'tgccaagatctgaaaaagca-3'
R-5' cctcttggccagagacttga-3'
99 FJ200440.1 59 300
300
0.98 -0.38
ACTB F-5'ggacctgacggactacctcatg-3'
R-5'ggccatctcctgctcgaagt-3'
136 U39357 60 300
300
0.989 -3.32
SDHA F-5'catccactacatgacggagca-3'
R-5'atcttgccatcttcagttctgcta-3'
90 AY970969 60 300
300
0.990 -3.36
G6PDH F-5'tgacctatggcaaccgatacaa-3'
R-5' ccgcaaaagacatccaggat-3'
76 DQ377364 60 900
900
0.994 -3.33
UBC F-5 cgtcttaggggtggctgtta-3'
R-5'aaattggggtaaatggctaga-3'
90 NM_001009202 59 600
600
0.991 -3.51
  1. Genes, primers (F: forward and R: reverse), length of the amplicon and GenBank accession (acc.) numbers for the ovine sequences. Real-time RT-PCR conditions: annealing temperature (AT), primer concentrations (Nm), correlation coefficient (R2) and slope of the standard curve.