Changes in protein level of osteopontin in paraffin embedded kidney sections of the treatment group and the controls. A &B expression of OPN in the outer medulla in the TALH and few of collecting ducts in control and treatment groups respectively × 100. C &E show normal expression of OPN in control group. D &F show the increased intensity of staining in cellular content and luminal expression of OPN in treatment group. DAB counterstained with hematoxelyne × 400.