Fig. 1

Multiple alignment of esculentin-1PN’s amino acid sequences and homologs. The threshold for shading was 70%; similar residues are marked with a grey shadow, identical residues with a black shadow, and alignment gaps with “-.” The alignment was accompanied by labels indicating the signal peptide, acidic spacer peptide, and mature peptide. An arrow (↓) marks the predicted cleavage site for either the signal peptide or the mature peptide. The KR-propeptide convertase processing site is enclosed within a red box. The presence of two conserved cysteine residues in the mature peptide is indicated by an asterisk (“*”)