Table 3 Primer sequences for qRT-PCR analysis
Gene |  | Nucleotide Sequence (5’-3’) | Tm (℃) | Length (bp) | accession numbers | |
|---|---|---|---|---|---|---|
IL-1β | Forward | TCTGCATGAGCTTTGTGCAAG | 59.73 | 155 | NM_001302388.2 | |
Reverse | ACAGGGCAGACTCGAATTCAAC | 60.87 | ||||
IL-6 | Forward | CTTCTGGTGATGGCTACTG | 52.66 | 134 | AF518322.1 | |
Reverse | TTGCCGAGGATGTACTTAA | 50 | ||||
IL-8 | Forward | ACAGCAGTAACAACAACAAG | 50.18 | 117 | AB057440.1 | |
Reverse | GACCAGCACAGGAATGAG | 53.17 | ||||
IL-10 | Forward | CGTGGAGGAGGTGAAGAGTG | 55.4 | 178 | NM_214041.1 | |
Reverse | TTAGTAGAGTCGTCATCCTGGAAG | 55.6 | ||||
IL-18 | Forward | AGTAACCATATCTGTGCAGTGT | 53.95 | 155 | AF191088.1 | |
Reverse | TCTTATCACCATGTCCAGGAAC | 53.04 | ||||
TNF-α | Forward | CGCTCTTCTGCCTACTGCACTTC | 60.68 | 164 | JF831365.1 | |
Reverse | CTGTCCCTCGGCTTTGACATT | 57.77 | ||||
IFN-γ | Forward | GAGGTTCCTAAATGGTAGCTCTGG | 57.08 | 164 | S63967.1 | |
Reverse | TCTGACTTCTCTTCCGCTTTCTT | 55.55 | ||||
IL-2 | Forward | AGCCATTGCTGCTGGATTT | 55.05 | 107 | FJ543109.1 | |
Reverse | AGCCTGCTTGGGCATGTAA | 57.34 | ||||
PD-1 | Forward | GCGGAATGTCAAGGAAACC | 54.31 | 150 | NM_001097431.1 | |
Reverse | CTGTACCCGTGGAGGAGGA | 59.14 | ||||
PD-L1 | Forward | AATGGCGAGGAAGACCTGAA | 56.24 | 137 | NM_001025221.1 | |
Reverse | CAGCAGTAAACCCCTGCATCT | 57.62 | ||||
CD3 | Forward | GTTTGCTGATGGTGGTGTA | 52.4 | 144 | NM_214227.1 | |
Reverse | TGGGCTCATAGTCTGGATT | 52.45 | ||||
CD4 | Forward | AGCCTCAGTTACCGAGTT | 52.74 | 138 | KU248481.1 | |
Reverse | ATCCTCTTGTCTTCCACTTC | 51.14 | ||||
CD8 | Forward | GTTACATCTCTGGTTACAAGG | 49.89 | 139 | NM_001001907.1 | |
Reverse | AAGAAGACGGACATGAAGTT | 50.65 | ||||
PI3K | Forward | TTGCTACAATCAATCGCCAGGAGAC | 59.32 | 147 | XM_021086552.1 | |
Reverse | CTTCCCGTTGTTGCCATCGTTTG | 59.67 | ||||
Akt | Forward | GGACGGGCACATCAAGATCACTG | 60.84 | 126 | NM_001159776.1 | |
Reverse | TAGTCGTTGTCCTCCAGCACCTC | 61.16 | ||||
mTOR | Forward | AGTACCTCCAGGACACCATGAACC | 60.88 | 108 | XM_003127584.6 | |
Reverse | CAGACCTCACAGCCACAGAAAGC | 60.97 | ||||
GAPDH | Forward | GGCACAGTCAAGGCGGAGAAC | 61.89 | 105 | NM_001206359.1 | |
Reverse | AGCACCAGCATCACCCCATTTG | 60.99 | ||||