Table 1 Primers used for RT-qPCR
From: Quercetin inhibition of porcine intestinal alpha coronavirus in vitro and in vivo
Primer | Sequence (5’→3’) |
|---|---|
SADS-CoV N-F | CTGACTGTTGTTGAGGTTAC |
SADS-CoV N-R | TCTGCCAAAGCTTGTTTAAC |
TLR3-F | AATCATTACCAATCACACTTAAGCTGTTA |
TLR3-R | CAAAACCAGCAACACGACTTTC |
IL6-F | TGAACTCCTTCTCCACAAGC |
IL6-R | GCGGCTACATCTTTGGAATC |
IL8-F | CTGGCGGTGGCTCTCTTC |
IL8-R | CCTTGGCAAAACTGCACCTT |
TNF-α-F | GCCACCACGCTCTTCTGTCTG |
TNF-α-R | AGGGGTCCTTGGGGAACTCTT |
P53-F | CGCTTCGAGATGTTCCGAGA |
P53-R | TTCAGGTGGCTGGAGTG |
GAPDH-F | CCTTCCGTGTCCCTACTGCCAAC |
GAPDH-R | GACGCCTGCTTCACCACCTTCT |