Table 1 The respective genes and their specific primers, expected PCR product sizes and the amplification conditions
Target gene | Primer | PCR product size (bp) | Reaction conditions | Reference |
|---|---|---|---|---|
12S rDNA | F: 5’-GACACA GGAGGTAGTGA CAAG-3’ R: 5’-CTAAGA ATTTCA CCTCTG ACAGT-3’ | 320 | 94 °C 5 min, 35× (94 °C 30 s, 52 °C 45 s, 72 °C 45 s), 72 °C 7 min | [39] |
16S rDNA | F: 5’- TTA AAT TGC TGT RGT ATT -3’ R: 5’- CCG GTC TGA ACT CASAWC -3’ | 455 | 94 °C 5 min, 35× (94 °C 30 s, 48 °C 45 s, 72 °C 45 s), 72 °C 7 min | [38] |
ITS2 | F: 5’-GAGTCTGCCAAATCCTTA C- 3’ R: 5’-TCCTCTACAGCTGCTTCG-3’ | 1200 | 94 °C 5 min, 35× (94 °C 30 s, 55 °C 45 s, 72 °C 90 s), 72 °C 7 min | [38] |