Table 1 Lists of primers used to perform qRT-PCR
Target gene | NCBI ID | PCR product size (bp) | Sequence (5’ → 3’) | Cytokine categories [reference] |
|---|---|---|---|---|
IFN-γ | NM_001009873.1 | 147 | F-ATGTAGCAGATGGTGGGTCG | Pro-inflammatory [61] |
R-TCCTTTGAATGCGCTGGTCA | ||||
IL1B | NM_001077414.1 | 161 | F-AAGACGGGAAACCCACCCTA | Pro-inflammatory [62] |
R-TGCTTGAGAGGTGCTGATGT | ||||
IL2 | XM_023252215.1 | 270 | F-AGAGCTTTCTATCAGCCTCTCT | Pro-inflammatory [63] |
R-GGCCTTCTTGGGCACGTAAA | ||||
IL4 | NM_001043339.1 | 133 | F-GAGAAACGACTCGTGCATGG | Anti-inflammatory [62] |
R-GGTGGAGCAGTTGTGATGTG | ||||
IL8 (CXCL8) | NM_001009281.1 | 172 | F-GACCCCAAGCAAAAGTGGGT | Pro-inflammatory [64] |
R-ACTGCATGAAGTGCTGAAGTG | ||||
IL10 | NM_001009209.1 | 156 | F-TCAAACCAAGGACGAGCTGC | Anti-inflammatory [62] |
R-TGTTTGATGTCTGGGTCCTCG | ||||
IL12A | NM_001009833.1 | 117 | F-CACACCAAGCCCAGGAATGT | Pro-inflammatory [65] |
R-TCGGAAGTGCAGGGGTAAAA | Â | |||
IL12B | NM_001077413.1 | 185 | F-TGTCAAAAGCAGCAGAGGCT | Pro-inflammatory [65] |
R-GAATAGCGTCCACCACGACT | ||||
TNF-α | NM_001009835.1 | 81 | F-CCCACATGGCCTGCAACTAA | Pro-inflammatory [62] |
R-GCTACTGGCTTGTCACTCGG | ||||
GAPDH | NM_001009307 | 101 (genomic 173) | F-AGTATGATTCCACCCACGGCA | Not applicable |
R-GATCTCGCTCCTGGAAGATGGT |