Biochemical and molecular characterization of Campylobacter fetus isolates from bulls subjected to bovine genital campylobacteriosis diagnosis in Spain

Table 2 Comparison of subspecies identification results using the different techniques (biochemistry, PCR, WGS, and gene sequencing) for discrepant C. fetus isolates

Sample	Phenotypic identification tests				PCR 1 & 2^a	WGS^b	PCR-3^c	Gene sequence % identity^d
Sample	H₂S 72 h	H₂S 5 days	Glycine 1%	Phenotype	PCR 1 & 2^a	WGS^b	PCR-3^c	ATP_BP	*yckJ*	*yckK*	*cysK*
Sal3	+	+	+	Cff	Cfv	Cfv (97.63)	Cfvi	100	100	100	100
C3.46	+	+	+	Cff	Cfv	Cfv (94.86)	Cfvi	100	100	100	100
C3.52	+	+	+	Cff	Cfv	Cfv (95.01)	Cfvi	100	100	100	100
C2.6	+	+	+	Cff	Cfv	Cfv (95.36)	Cfvi	100	100	100	100
C3.4	+	+	+	Cff	Cfv	Cfv (95.74)	Cfvi	100	100	100	100
C3.10	+	+	+	Cff	Cfv	Cfv (95.34)	Cfvi	100	100	100	100
C1.43	-	-	-	Cfv	Cfv	Cfv (95.89)	Cfvi	100	100	99.87	100
C3.5	-	-	-	Cfv	Cfv	Cfv (95.48)	Cfvi	100	100	100	100

Cff Camylobacter fetus subsp. fetus, Cfv Camylobacter fetus subsp. venerealis, Cfvi Camylobacter fetus subsp. venerealis bv. intermedius
^aPCRs targeting the nahE and the ISCfe for C. fetus subspecies identification [14, 15]
^bThe number in brackets indicates the KamerFinder query coverage of these isolates compared to the reference strain CfviADRI545
^cPCR targeting the L-Cysteine transporter genes for Cfv biovars differentiation [16]
^dPercentage identity result of these genes compared to the reference strain Cff 82–40

ISSN: 1746-6148