Biochemical and molecular characterization of Campylobacter fetus isolates from bulls subjected to bovine genital campylobacteriosis diagnosis in Spain

Pena-Fernández, Nerea; Kortabarria, Nekane; Hurtado, Ana; Ocejo, Medelin; Fort, Marcelo; Pérez-Cobo, Iratxe; Collantes-Fernández, Esther; Aduriz, Gorka

doi:10.1186/s12917-024-03970-8

Table 1 Phenotypic and molecular identification of C. fetus control and field isolates

From: Biochemical and molecular characterization of Campylobacter fetus isolates from bulls subjected to bovine genital campylobacteriosis diagnosis in Spain

	Sample	Phenotypic identification tests				Molecular (PCR) identification tests
	Sample	H₂S 72 h^a	H₂S 5 days^b	Glycine 1%	Phenotype^c	PCR-1^d	PCR-2^e	PCR-3^f
Control strains	Cfv ATCC 25922	-	-	-	Cfv	Cfv	Cfv	Cfv
	*Cfvi3*	-	+	+	*Cff*	*Cfv*	*Cfv*	Cfvi
	Cff1	-	+	+	Cff	Cff	Cff	nd
	Cff2	-	-	+	Cff	Cff	Cff	nd
	Cff C0075	-	-	+	Cff	Cff	Cff	nd
	Cff C0011	+	+	+	Cff	Cff	Cff	nd
	Cff C0023	+	+	+	Cff	Cff	Cff	nd
	Cff C0024	+	+	+	Cff	Cff	Cff	nd
	Cff C0037	+	+	+	Cff	Cff	Cff	nd
	Cff C0048	+	+	+	Cff	Cff	Cff	nd
	Cff C0054	+	+	+	Cff	Cff	Cff	nd
	Cff C0173	+	+	+	Cff	Cff	Cff	nd
	Cff C0228	+	+	+	Cff	Cff	Cff	nd
	C. hyo hyo	nd	nd	+	No C. fetus	No C. fetus	No C. fetus	nd
*C. fetus* field isolates	SAL 2	-	-	+	Cff	Cff	Cff	nd
	C1. 54	+	+	+	Cff	Cff	Cff	nd
	C2.39	+	+	+	Cff	Cff	Cff	nd
	C2.6	+	+	+	*Cff*	*Cfv*	*Cfv*	Cfvi
	C3.10	+	+	+	*Cff*	*Cfv*	*Cfv*	Cfvi
	C3.4	+	+	+	*Cff*	*Cfv*	*Cfv*	Cfvi
	C3.46	+	+	+	*Cff*	*Cfv*	*Cfv*	Cfvi
	C3.52	+	+	+	*Cff*	*Cfv*	*Cfv*	Cfvi
	SAL 3	+	+	+	*Cff*	*Cfv*	*Cfv*	Cfvi
	C1. 30	-	-	-	Cfv	Cfv	Cfv	Cfv
	C1. 49	-	-	-	Cfv	Cfv	Cfv	Cfv
	C1. 55	-	-	-	Cfv	Cfv	Cfv	Cfv
	C1. 70	-	-	-	Cfv	Cfv	Cfv	Cfv
	C2.30	-	-	-	Cfv	Cfv	Cfv	Cfv
	C3.49	-	-	-	Cfv	Cfv	Cfv	Cfv
	C1. 43	-	-	-	*Cfv*	Cfv	Cfv	*Cfvi*
	C1. 59	-	-	-	Cfv	Cfv	Cfv	Cfv
	C1. 84	-	-	-	Cfv	Cfv	Cfv	Cfv
	C3.5	-	-	-	*Cfv*	Cfv	Cfv	*Cfvi*
	SAL 5	-	-	-	Cfv	Cfv	Cfv	Cfv
	C1. 41	-	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 56	-	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.1	-	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.31	-	+	-	Cfvi	Cfv	Cfv	Cfvi
	C3.63	-	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 32	-	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 67	-	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 75	-	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 24	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 65	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 69	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 73	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 85	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1.91	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1.96	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.12	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.17	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.21	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.32	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.7	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.8	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C3.6	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 40	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 58	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 60	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 76	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 87	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1. 88	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C1.92	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.14	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.15	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.2	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.22	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.3	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.33	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.35	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.36	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C2.37	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C3.15	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C3.17	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C3.44	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	C3.62	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	SAL 1	+	+	-	Cfvi	Cfv	Cfv	Cfvi
	SAL 4	+	+	-	Cfvi	Cfv	Cfv	Cfvi

Strains with discordant phenotypic and molecular identification results have been marked in bold
nd not determined, Cff Camylobacter fetus subsp. fetus, Cfv Camylobacter fetus subsp. venerealis, Cfvi Camylobacter fetus subsp. venerealis bv. intermedius, C. hyo hyo Campylobacter hyointestinalis subsp. hyointestinalis
^{a, b}H₂S production by lead acetate strips at 72 h and 5 days
^cPhenotype results are based on the glycine tolerance test and H₂S production after 5 days
^dPCR-1: PCR described by Abril et al., for Cfv and Cff differentiation [15]
^ePCR-2: Real time PCR described by van der Graaf et al., for Cfv and Cff differentiation [14]
^fPCR-3: PCR described by Farace et al., for Cfv and Cfvi differentiation [16]

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