Fig. 2

Representative graphs of gross, histopathological and IHC examinations of the necropsied CCPP naturally infected goats; A Peracute form represented by lung congestion, severe pulmonary edema and white-colored foamy fluid in the ‎trachea; B Acute form showing marbling appearance and hepatization of the lungs, hydrothorax, and fibrinous pleuritis C Chronic form characterized by unilateral pleural adhesion (arrowhead), lung hepatization and severe hydrothorax; D Photomicrograph of pulmonary tissue showing fibrin threads deposition in a bronchiole (arrowhead) and surrounding alveoli besides obliteration of another bronchiole with inflammatory cells (asterisk) (H&E, bar=200µm); E Consecutive section of (D) stained with anti-Mccp antibody showing intense brown positive staining in the fibrin deposits, inflammatory cells obliterating the bronchioles and alveoli, and the peribronchial lymphoid aggregates (DAB staining, Hematoxylin counterstaining, bar=200µm)