Table 1 Oligonucleotide primers sequence, annealing temperature and PCR product size of the studied genes
Gene | Primer | Product length (bp) | Annealing Temperature (°C) | Accession number | Source |
|---|---|---|---|---|---|
IGF-I | F:5-CTTCCGGAGCTGTGATCTGA-3 R:5-TGTACTTCCTTCTGAGCCTTGG-3 | 123 | 59 | MZ491851.1 | Current study |
ACACA | F:5- TTCCAATGTCTGCTTGTCCGT-3 R:5- GCTGAGCAGAGTCGAAGAACA-3 | 190 | 60 | KP236453.1 | Current study |
SCD | F:5- ATGGTTGAGCCCCAGTGATG-3 R:5- TCCAGCCTTGCGTAGAGTTG-3 | 150 | 60 | XM_010971047.2 | Current study |
FASN | F:5- GGTGGACTCGCTGAAGAACA-3 R:5- ATACCAGGACGCACCGAATC-3 | 149 | 62 | XM_010950631.2 | Current study |
BTN1A1 | F:5- ACGTCCTACACACGTTCACC-3 R:5- CCAAAACCTCTCTCGGCGAT-3 | 154 | 60 | XM_010980175.2 | Current study |
SOD 3 | F:5-GACACCTCTCCAAAAGCCCA-3 R:5-GCACATGGTTGGAGGCCTTA-3 | 169 | 60 | XM_031436563.1 | Current study |
CAT | F:5-GATGAGAAGCCGAAGAACGC-3 R:5-ATGCTTGGCCTCATAGGCAG-3 | 136 | 60 | XM_011000575.2 | Current study |
GPX | F:5-CACCTGGTCTCCAGTATGCC-3 R:5-TCGATGTCAGGCTCGATGTC-3 | 129 | 59 | XM_031470314.1 | Current study |
PRDX2 | F:5-CCAAACACAACTAGGCTGGC-3 R:5-CCTTTAGGGCCATGGACTGT-3 | 137 | 58 | XM_010993804.2 | Current study |
GAPDH | F:5-TCGATCCCCCAACACACTTG-3 R:5-TGATGGTGCATGACAAGGCA-3 | 106 | 58 | XM_010990867.2 | Current study |