Table 1 Primers used in PCR to detect targeted genes
From: Serological investigation and isolation of Salmonella abortus equi in horses in Xinjiang
Gene | Primer oligonucleotide sequences (5′-3′) (forward/reverse) | Amplicon size (bp) | Annealing temperature (℃) |
|---|---|---|---|
16Â S | AGAGTTTGATCCTGGCTCA/ACCTGTCACCCGATGTACC | 1460 | 56 |
FliC | CCAGACTCAGTTCAACGG/AAACCGCCATCAATAGTC | 588 | 52 |
invA | GTCACCGTGGTCCAGTTT/CTCTTTCCAGTACGCTTCG | 175 | 52 |
fliB | GTAGGATCCTCCATTFGCGCGCAGACCACTGAAGGCGC/ TAACTCGAGTTTCGGTCGCGTAGTCGGAATCTTCGA | 1195 | 62 |