Table 1 Concordance analysis between dRAP-Cas12a/Cas13a-Fluor assay and real-time PCR methods
| Â | FHV-1 qPCR | Total | Sensitivity (%) | Specificity (%) | Kappa | ||
|---|---|---|---|---|---|---|---|
+ | - | ||||||
dRPA-Cas12a/13a-Fluor assay | + | 19 | 0 | 19 | 100 | 100 | 1.00 |
- | 0 | 37 | 37 | ||||
Total | 19 | 38 | 56 | ||||
| Â | FCV RT-qPCR | Â | |||||
+ | - | Â | |||||
dRPA-Cas12a/13a-Fluor assay | + | 21 | 0 | 21 | 100 | 100 | 1.00 |
- | 0 | 35 | 35 | ||||
Total | 21 | 35 | 56 | ||||