Fig. 5
Target sequences recognized by primers and oligonucleotides. The recognition sites of RPA primers, crRNA oligonucleotides, and the reference real-time PCR primers for highly conservative FHV-1 TK gene (66,744 ∼ 66,898 nt, CH-B strain genome) and FCV ORF1 gene (2418 ∼ 2550 nt, SMU-B5-2020 strain genome) are illustrated, respectively. The TK and OFR-1 gene fragments are cloned into pMDTM19-T plasmid for later target DNAs preparation