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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Ultrasensitive and visual detection of Feline herpesvirus type-1 and Feline calicivirus using one-tube dRPA-Cas12a/Cas13a assay

Fig. 3

Sensitivity of dRPA-Cas12a/Cas13a assay. Serial dilutions (107-10− 2) of FHV-1 and FCV plasmid DNA were tested using optimized dRPA-Cas12a/Cas13a assay. The limit of detection (LOD) for FHV-1 and FCV were at 10− 1 and 100 dilution, respectively, by fluorescence (A) and lateral flow dipstick (C) modality. (B) The detection capabilities for FHV-1 and FCV were nearly parallel in serial dilutions, while the LOD for FHV-1 (10-1 dilution) was lower than FCV (100 dilution). The statistical significance was indicated by asterisks, ***P < 0.001. NC: negative control (DNase/RNase-free water)

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BMC Veterinary Research

ISSN: 1746-6148

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