Table 3 Primers used for qPCR
Gene | Sequence (5’ − 3’) | |
|---|---|---|
fimD-F | CGCGGCGAAAGTTATTTCAA | |
fimD-R | CCACGGACGCGGTATCC | |
flgG-F | GCGCCGGACGATTGC | |
flgG-R | CCGGGCTGGAAAGCATT | |
prot6E-F | GAACGTTTGGCTGCCTATGG | |
prot6E-R | CGCAGTGACTGGCATCAAGA | |
csgA-F | AATGCCACCATCGACCAGTG | |
csgA-R | CAAAACCAACCTGACGCACC | |
csgD-F | GCCTCATATTAACGGCGTG | |
csgD-R | AGCGGTAATTTCCTGAGTGC | |
bcsA-F | GCCCAGCTTCAGAATATCCA | |
bcsA-R | TGGAAGGGCAGAAAGTGAAT | |
ompR-F | TGTGCCGGATCTTCTTCCA | |
ompR-R | CTCCATCGACGTCCAGATCTC | |
hflK-F | AGCGCGGCGTTGTGA | |
hflK-R | TCAGACCTGGCTCTACCAGATG | |
tatA-F | AGTATTTGGCAGTTGTTGATTGTTG | |
tatA-R | ACCGATGGAACCGAGTTTTTT | |
lrp-F | TTAATGCCGCCGTGCAA | |
lrp-R | GCCGGAAACCAAATGACACT | |
sipA-F | CAGGGAACGGTGTGGAGGTA | |
sipA-R | AGACGTTTTTGGGTGTGATACGT | |
sipB-F | GCCACTGCTGAATCTGATCCA | |
sipB-R | CGAGGCGCTTGCTGATTT | |
pipB-F | GCTCCTGTTAATGATTTCGCTAAAG | |
pipB-R | GCTCAGACTTAACTGACACCAAACTAA | |
invH-F | CCCTTCCTCCGTGAGCAAA | |
invH-R | TGGCCAGTTGCTCTTTCTGA | |
mgtC-F | CGAACCTCGCTTTCATCTTCTT | |
mgtC-R | CCGCCGAGGGAGAAAAAC | |
sodC-F | CACATGGATCATGAGCGCTTT | |
sodC-R | CTGCGCCGCGTCTGA | |
orf245-F | CAGGGTAATATCGATGTGGACTACA | |
orf245-R | GCGGTATGTGGAAAACGAGTTT | |
rfbH-F | ACGGTCGGTATTTGTCAACTCA | |
rfbH-R | TCGCCAACCGTATTTTGCTAA | |
xthA-F | CGCCCGTCCCCATCA | |
xthA-R | CACATCGGGCTGGTGTTTT | |
mrr1-F | CCATCGCTTCCAGCAACTG | |
mrr1-R | TCTCTACCATGAACCCGTACAAATT | |
16Â S rRNA-F 16Â S rRNA-R | CCAGGGCTACACACGTGCTA TCTCGCGAGGTCGCTTCT | |