Table 2 Primers used for constructing the mutant and the complemented strain
Primer | Sequence (5’ − 3’) |
|---|---|
P1: | TGGGCATGGTCAATATTCGGGATCAGGTCATTCCGGTGATTGATTTGCCAGCGGTAGCGTGTGTAGGCTGGAGCTGCTTCG |
P2: | TCATTGGCGCTGCCGGAAAGCGAGGAGTGGATAACGACCGGGATCTTTTTCAGCCGTTCCATATGAATATCCTCCTTAG |
P3: | TGAACTGCTGTTATTCCGTCTTGG |
P4: | ATCAGTGGTCCCTGGCTGTTTG |
P5: | GTTAGGATGGACAATTTTCAGAAAGATA |
P6: | TCAGGCGCTTTTGCGGCTGATCAGTGGT |