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Table 1 Bacterial strains and plasmids used in this study

From: CheV enhances the virulence of Salmonella Enteritidis, and the Chev-deleted Salmonella vaccine provides immunity in mice

Strains

Relevant characteristics

Sources

C50336

Wild-type S. enteritidis

This study

ΔcheV::cat

a first recombination strain

This study

ΔcheV

a second recombination strain

This study

ΔcheV + cheV

ΔcheV-complemented strain

This study

Plasmids

Characteristics

Sources

pKD3

FRT-Cm-FRT cassette (CbR CmR)

invitrigen

pKD46

Apr, containing the Red recombinase of λ phage

invitrigen

pCP20

FLP recombinase, temperature sensitive replication (AMR)

invitrigen