Fig. 2
From: Attenuated vaccine PmCQ2Δ4555–4580 effectively protects mice against Pasteurella multocida infection
The identification of PmCQ2Δ4555–4580. A: PCR identification of PmA. B: Whole genome resequencing of PmCQ2Δ4555–4580. C: PCR confirmation of missed genes in PmCQ2Δ4555–4580. D: RT-qPCR confirmation of missed genes in PmCQ2Δ4555–4580. M1: 2000 DNA marker. M2: 5000 DNA marker. Lanes 1, 4, 8, 10, 12, 14, 16, and 18: PmCQ2. Lanes 2, 5, 7, 9, 11, 13, 15, and 17: PmCQ2Δ4555–4580. Lanes 3 and 6: E. coli. K: The specific primer (KMT1-F/R) of Pm. A: The specific primer (CapA-F/R) of PmA. The data of D were pooled from three independent experiments with 3 replicates per group, and were analyzed by multiple comparative analysis, and expressed as means ± SD